ENSG00000121690

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000241051 ENSG00000121690 HEK293_OSMI2_2hA HEK293_TMG_2hB DEPDC7 protein_coding protein_coding 6.355359 3.807214 8.97535 0.3900808 0.5863788 1.235055 5.6470714 3.76715794 7.5304112 0.39794104 0.68353649 0.9973421 0.90228333 0.98873333 0.83636667 -0.15236667 3.491746e-07 3.491746e-07 FALSE TRUE
ENST00000427755 ENSG00000121690 HEK293_OSMI2_2hA HEK293_TMG_2hB DEPDC7 protein_coding retained_intron 6.355359 3.807214 8.97535 0.3900808 0.5863788 1.235055 0.2461222 0.00000000 0.5636024 0.00000000 0.05602050 5.8419791 0.03384583 0.00000000 0.06393333 0.06393333 1.090160e-05 3.491746e-07 FALSE TRUE
ENST00000532078 ENSG00000121690 HEK293_OSMI2_2hA HEK293_TMG_2hB DEPDC7 protein_coding retained_intron 6.355359 3.807214 8.97535 0.3900808 0.5863788 1.235055 0.4596312 0.04005581 0.8813360 0.02007007 0.08674092 4.1543598 0.06362917 0.01126667 0.09973333 0.08846667 2.003486e-04 3.491746e-07 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000121690 E001 0.000000       11 33015876 33015877 2 +      
ENSG00000121690 E002 5.433121 0.0928360468 6.464196e-01 7.583318e-01 11 33015878 33015910 33 + 0.834 0.734 -0.398
ENSG00000121690 E003 24.830846 0.0082647620 2.166460e-01 3.463500e-01 11 33015911 33016028 118 + 1.440 1.333 -0.370
ENSG00000121690 E004 0.000000       11 33016164 33016585 422 +      
ENSG00000121690 E005 71.413443 0.0094190015 2.468659e-01 3.822695e-01 11 33025659 33026049 391 + 1.876 1.804 -0.243
ENSG00000121690 E006 5.472317 0.0031018093 1.170621e-05 7.658233e-05 11 33026050 33026717 668 + 0.979 0.272 -3.292
ENSG00000121690 E007 44.618160 0.0006258198 8.887055e-01 9.328217e-01 11 33027686 33027813 128 + 1.654 1.644 -0.033
ENSG00000121690 E008 6.926118 0.0142490239 1.150678e-05 7.540646e-05 11 33027814 33028294 481 + 1.059 0.363 -3.001
ENSG00000121690 E009 59.961975 0.0005503559 4.048716e-01 5.485550e-01 11 33028603 33028792 190 + 1.790 1.750 -0.133
ENSG00000121690 E010 58.714230 0.0024154651 8.738517e-01 9.229198e-01 11 33031378 33031589 212 + 1.769 1.757 -0.038
ENSG00000121690 E011 39.809847 0.0006583029 2.928547e-01 4.338714e-01 11 33032336 33032478 143 + 1.630 1.569 -0.206
ENSG00000121690 E012 47.721585 0.0024140752 9.641408e-02 1.849788e-01 11 33032668 33032793 126 + 1.647 1.732 0.289
ENSG00000121690 E013 51.880728 0.0084414117 1.978460e-02 5.103041e-02 11 33032889 33032967 79 + 1.657 1.795 0.467
ENSG00000121690 E014 60.432500 0.0005407076 1.044448e-03 4.112643e-03 11 33033262 33033582 321 + 1.721 1.859 0.467