ENSG00000121481

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000367509 ENSG00000121481 HEK293_OSMI2_2hA HEK293_TMG_2hB RNF2 protein_coding protein_coding 16.25245 5.634006 27.57422 0.5687495 0.6088916 2.289052 0.9250362 0.2177942 1.503258 0.02598561 0.1523598 2.7318548 0.05385417 0.03956667 0.05436667 0.0148000 5.179270e-01 3.613073e-07 FALSE TRUE
ENST00000367510 ENSG00000121481 HEK293_OSMI2_2hA HEK293_TMG_2hB RNF2 protein_coding protein_coding 16.25245 5.634006 27.57422 0.5687495 0.6088916 2.289052 5.3914120 4.4730865 8.674939 0.71439931 0.1694006 0.9540233 0.45722083 0.78583333 0.31496667 -0.4708667 3.613073e-07 3.613073e-07 FALSE TRUE
ENST00000453650 ENSG00000121481 HEK293_OSMI2_2hA HEK293_TMG_2hB RNF2 protein_coding protein_coding 16.25245 5.634006 27.57422 0.5687495 0.6088916 2.289052 7.5215598 0.2485489 13.004809 0.24854891 0.3668657 5.6535731 0.33315417 0.05066667 0.47170000 0.4210333 2.567788e-02 3.613073e-07 FALSE FALSE
MSTRG.2735.1 ENSG00000121481 HEK293_OSMI2_2hA HEK293_TMG_2hB RNF2 protein_coding   16.25245 5.634006 27.57422 0.5687495 0.6088916 2.289052 1.7135788 0.5223348 3.375583 0.10608344 0.4342722 2.6689984 0.10934167 0.09256667 0.12186667 0.0293000 5.953235e-01 3.613073e-07 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000121481 E001 6.3290795 0.0029721518 1.517348e-01 2.640225e-01 1 185045340 185045429 90 + 0.829 0.688 -0.569
ENSG00000121481 E002 11.1683902 0.0017303969 6.864676e-02 1.411216e-01 1 185045430 185045464 35 + 1.048 0.908 -0.520
ENSG00000121481 E003 17.2000882 0.0010771213 4.178595e-02 9.433734e-02 1 185045465 185045525 61 + 1.220 1.101 -0.426
ENSG00000121481 E004 16.3990981 0.0011110579 2.200914e-02 5.569669e-02 1 185045526 185045535 10 + 1.207 1.054 -0.549
ENSG00000121481 E005 19.9023070 0.0009031606 2.049541e-03 7.379492e-03 1 185045536 185045557 22 + 1.295 1.078 -0.771
ENSG00000121481 E006 58.8839110 0.0023073719 1.426260e-04 7.148544e-04 1 185045558 185045649 92 + 1.738 1.592 -0.495
ENSG00000121481 E007 0.1451727 0.0435292131 1.000000e+00   1 185046096 185046295 200 + 0.066 0.000 -7.184
ENSG00000121481 E008 1.6803446 0.4524607144 8.347153e-01 8.965292e-01 1 185071580 185071696 117 + 0.392 0.362 -0.175
ENSG00000121481 E009 91.7648352 0.0003304374 5.548199e-06 3.909577e-05 1 185087552 185087640 89 + 1.923 1.808 -0.388
ENSG00000121481 E010 2.6858853 0.0743162700 6.355851e-02 1.326215e-01 1 185091565 185091578 14 + 0.580 0.215 -2.123
ENSG00000121481 E011 154.1715740 0.0002873479 1.953495e-08 2.283340e-07 1 185091579 185091739 161 + 2.143 2.039 -0.348
ENSG00000121481 E012 0.3030308 0.3678985264 1.000000e+00   1 185091740 185092006 267 + 0.124 0.000 -8.191
ENSG00000121481 E013 216.7306247 0.0203291729 4.120404e-02 9.325809e-02 1 185093061 185093276 216 + 2.281 2.215 -0.220
ENSG00000121481 E014 246.3016566 0.0005123008 1.221473e-03 4.713337e-03 1 185098072 185098344 273 + 2.321 2.324 0.007
ENSG00000121481 E015 121.0101358 0.0002970369 9.532016e-01 9.747235e-01 1 185099791 185099879 89 + 1.996 2.083 0.289
ENSG00000121481 E016 119.2734707 0.0003034882 7.069481e-01 8.050032e-01 1 185099880 185099962 83 + 1.993 2.068 0.251
ENSG00000121481 E017 507.7512326 0.0116964875 2.883760e-08 3.256943e-07 1 185100200 185102603 2404 + 2.545 2.845 1.000