ENSG00000121281

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000254235 ENSG00000121281 HEK293_OSMI2_2hA HEK293_TMG_2hB ADCY7 protein_coding protein_coding 1.562819 2.290896 2.015737 0.1362692 0.09423356 -0.1837485 0.43889038 0.4367742 0.6397433 0.04235355 0.05902749 0.5403238 0.30902917 0.1923333 0.3167333 0.12440000 1.387842e-01 9.473879e-05 FALSE TRUE
ENST00000564044 ENSG00000121281 HEK293_OSMI2_2hA HEK293_TMG_2hB ADCY7 protein_coding protein_coding 1.562819 2.290896 2.015737 0.1362692 0.09423356 -0.1837485 0.08036078 0.0000000 0.2745938 0.00000000 0.27459380 4.8308323 0.05092083 0.0000000 0.1289000 0.12890000 8.133017e-01 9.473879e-05 FALSE FALSE
ENST00000564965 ENSG00000121281 HEK293_OSMI2_2hA HEK293_TMG_2hB ADCY7 protein_coding protein_coding 1.562819 2.290896 2.015737 0.1362692 0.09423356 -0.1837485 0.38893212 0.5996541 0.6557359 0.09207345 0.14849643 0.1269591 0.23295417 0.2588333 0.3285000 0.06966667 7.998214e-01 9.473879e-05 FALSE FALSE
ENST00000566433 ENSG00000121281 HEK293_OSMI2_2hA HEK293_TMG_2hB ADCY7 protein_coding protein_coding 1.562819 2.290896 2.015737 0.1362692 0.09423356 -0.1837485 0.47019210 1.0414543 0.2184479 0.10394217 0.06429000 -2.2024492 0.29310833 0.4523333 0.1112000 -0.34113333 9.473879e-05 9.473879e-05 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000121281 E001 0.8010032 0.116021999 4.143250e-01 5.577375e-01 16 50246137 50246147 11 + 0.342 0.185 -1.174
ENSG00000121281 E002 5.2963011 0.003220097 4.608807e-01 6.008662e-01 16 50246148 50246203 56 + 0.845 0.741 -0.414
ENSG00000121281 E003 0.0000000       16 50266551 50266563 13 +      
ENSG00000121281 E004 0.0000000       16 50266564 50266587 24 +      
ENSG00000121281 E005 0.0000000       16 50266588 50266680 93 +      
ENSG00000121281 E006 0.0000000       16 50274131 50274152 22 +      
ENSG00000121281 E007 0.0000000       16 50274153 50274156 4 +      
ENSG00000121281 E008 0.0000000       16 50274157 50274198 42 +      
ENSG00000121281 E009 0.0000000       16 50279527 50279704 178 +      
ENSG00000121281 E010 0.2998086 0.028783321 7.752411e-01   16 50287882 50287910 29 + 0.146 0.102 -0.598
ENSG00000121281 E011 0.2998086 0.028783321 7.752411e-01   16 50287911 50287911 1 + 0.146 0.102 -0.598
ENSG00000121281 E012 9.3802287 0.002529293 8.320861e-01 8.947553e-01 16 50287912 50288076 165 + 1.009 0.977 -0.117
ENSG00000121281 E013 13.0040278 0.020393483 8.805907e-01 9.274098e-01 16 50288077 50288350 274 + 1.140 1.113 -0.098
ENSG00000121281 E014 0.1472490 0.042844238 3.382840e-01   16 50289221 50289313 93 + 0.146 0.000 -10.249
ENSG00000121281 E015 9.0924405 0.017117586 7.784638e-01 8.574580e-01 16 50290457 50290660 204 + 1.009 0.965 -0.162
ENSG00000121281 E016 10.1402408 0.001960222 1.952709e-01 3.203121e-01 16 50291736 50291897 162 + 0.934 1.064 0.480
ENSG00000121281 E017 13.2158927 0.021762146 7.500184e-01 8.368023e-01 16 50292676 50292825 150 + 1.113 1.140 0.095
ENSG00000121281 E018 12.9379341 0.018938989 9.753902e-01 9.886454e-01 16 50293354 50293502 149 + 1.140 1.127 -0.046
ENSG00000121281 E019 12.2741600 0.001575869 1.861954e-01 3.089699e-01 16 50294640 50294751 112 + 1.025 1.145 0.434
ENSG00000121281 E020 16.2911369 0.001602343 7.524240e-02 1.519227e-01 16 50298904 50299031 128 + 1.114 1.260 0.521
ENSG00000121281 E021 12.8729063 0.001754168 2.079975e-01 3.360840e-01 16 50300715 50300819 105 + 1.041 1.154 0.405
ENSG00000121281 E022 9.8171290 0.009651504 9.859127e-01 9.952004e-01 16 50300820 50300873 54 + 1.009 1.002 -0.025
ENSG00000121281 E023 8.3168936 0.002146418 8.184637e-01 8.852574e-01 16 50301082 50301124 43 + 0.935 0.952 0.064
ENSG00000121281 E024 12.4767936 0.001418059 6.536271e-01 7.640572e-01 16 50301125 50301214 90 + 1.086 1.120 0.121
ENSG00000121281 E025 0.8544736 0.016137634 9.860542e-01 9.952954e-01 16 50301668 50301964 297 + 0.255 0.254 -0.010
ENSG00000121281 E026 8.3564335 0.002069933 7.320572e-01 8.236197e-01 16 50304360 50304393 34 + 0.935 0.964 0.111
ENSG00000121281 E027 14.1656816 0.001392561 8.369111e-01 8.980018e-01 16 50304394 50304516 123 + 1.152 1.162 0.033
ENSG00000121281 E028 9.1627315 0.001912133 1.771914e-01 2.975762e-01 16 50304517 50304551 35 + 0.892 1.033 0.527
ENSG00000121281 E029 8.4671748 0.006177463 3.196829e-01 4.627733e-01 16 50304925 50304959 35 + 0.892 1.000 0.403
ENSG00000121281 E030 13.3073061 0.001363734 5.023846e-01 6.384515e-01 16 50305503 50305586 84 + 1.100 1.153 0.191
ENSG00000121281 E031 12.9173286 0.001471668 3.790590e-02 8.717326e-02 16 50305777 50305849 73 + 0.991 1.185 0.700
ENSG00000121281 E032 12.5412224 0.001564027 1.577030e-01 2.720658e-01 16 50307050 50307147 98 + 1.025 1.154 0.464
ENSG00000121281 E033 13.0356974 0.001469277 3.825450e-01 5.267493e-01 16 50308327 50308411 85 + 1.072 1.146 0.265
ENSG00000121281 E034 0.3666179 0.029245822 7.783698e-01 8.573844e-01 16 50308412 50308666 255 + 0.146 0.102 -0.592
ENSG00000121281 E035 12.5198940 0.002744470 2.860037e-01 4.263656e-01 16 50308667 50308792 126 + 1.041 1.137 0.347
ENSG00000121281 E036 0.8544736 0.016137634 9.860542e-01 9.952954e-01 16 50308793 50309198 406 + 0.255 0.254 -0.010
ENSG00000121281 E037 13.0880641 0.001421882 5.626529e-01 6.902211e-01 16 50309548 50309646 99 + 1.100 1.145 0.161
ENSG00000121281 E038 3.8987986 0.004184967 8.192380e-02 1.625767e-01 16 50310454 50310686 233 + 0.477 0.741 1.171
ENSG00000121281 E039 5.1283263 0.003395744 5.922682e-01 7.148438e-01 16 50310687 50310880 194 + 0.820 0.740 -0.315
ENSG00000121281 E040 3.4632726 0.041848849 5.756775e-01 7.012176e-01 16 50311693 50311786 94 + 0.699 0.593 -0.456
ENSG00000121281 E041 3.7181315 0.007731231 5.361094e-02 1.155729e-01 16 50312036 50312191 156 + 0.820 0.529 -1.232
ENSG00000121281 E042 3.6450933 0.004554359 5.029597e-02 1.096885e-01 16 50312890 50313036 147 + 0.820 0.529 -1.233
ENSG00000121281 E043 0.1451727 0.043181552 3.381321e-01   16 50313104 50313957 854 + 0.146 0.000 -10.249
ENSG00000121281 E044 5.6766381 0.002849969 9.977526e-02 1.901393e-01 16 50313958 50314062 105 + 0.935 0.719 -0.843
ENSG00000121281 E045 0.0000000       16 50314063 50314291 229 +      
ENSG00000121281 E046 5.9857950 0.005920839 5.003128e-01 6.365811e-01 16 50314292 50314406 115 + 0.892 0.798 -0.363
ENSG00000121281 E047 5.3245773 0.050804509 7.588567e-01 8.433485e-01 16 50315014 50315138 125 + 0.764 0.811 0.188
ENSG00000121281 E048 40.3973920 0.006630097 5.255405e-07 4.628691e-06 16 50315359 50318135 2777 + 1.777 1.462 -1.072