ENSG00000120733

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000314358 ENSG00000120733 HEK293_OSMI2_2hA HEK293_TMG_2hB KDM3B protein_coding protein_coding 28.99659 27.76765 31.17319 1.411638 0.3334068 0.166844 13.384288 6.640471 18.89860 1.3550121 0.9382317 1.5075139 0.4553917 0.2356333 0.6069667 0.3713333 4.766901e-06 2.640993e-21 FALSE TRUE
ENST00000507996 ENSG00000120733 HEK293_OSMI2_2hA HEK293_TMG_2hB KDM3B protein_coding nonsense_mediated_decay 28.99659 27.76765 31.17319 1.411638 0.3334068 0.166844 8.678422 8.153430 7.16202 0.7843329 0.7215022 -0.1867958 0.3041125 0.2955333 0.2294333 -0.0661000 4.467309e-01 2.640993e-21 FALSE TRUE
ENST00000542866 ENSG00000120733 HEK293_OSMI2_2hA HEK293_TMG_2hB KDM3B protein_coding protein_coding 28.99659 27.76765 31.17319 1.411638 0.3334068 0.166844 2.835580 9.458293 0.00000 2.0415629 0.0000000 -9.8869606 0.1012333 0.3410667 0.0000000 -0.3410667 2.640993e-21 2.640993e-21 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000120733 E001 0.8878743 0.0131165474 1.604401e-02 4.283383e-02 5 138352685 138352762 78 + 0.420 0.001 -10.361
ENSG00000120733 E002 38.8989619 0.0170633071 3.728204e-07 3.383087e-06 5 138352763 138352987 225 + 1.737 1.350 -1.323
ENSG00000120733 E003 64.9062421 0.0003589908 4.881194e-11 9.083768e-10 5 138372674 138372792 119 + 1.912 1.672 -0.810
ENSG00000120733 E004 57.7135157 0.0003930601 1.194643e-09 1.744290e-08 5 138372793 138372841 49 + 1.860 1.627 -0.789
ENSG00000120733 E005 97.9454984 0.0003201155 3.343613e-14 1.017734e-12 5 138375093 138375206 114 + 2.083 1.859 -0.751
ENSG00000120733 E006 96.3699634 0.0002963420 6.901153e-11 1.251223e-09 5 138377720 138377810 91 + 2.063 1.880 -0.615
ENSG00000120733 E007 72.8702244 0.0003553847 6.010899e-09 7.737103e-08 5 138377811 138377825 15 + 1.945 1.757 -0.635
ENSG00000120733 E008 124.5609570 0.0002737709 3.424993e-16 1.376442e-14 5 138379584 138379679 96 + 2.183 1.974 -0.702
ENSG00000120733 E009 77.8111019 0.0003301545 1.849179e-09 2.607722e-08 5 138379680 138379708 29 + 1.974 1.786 -0.634
ENSG00000120733 E010 112.0920696 0.0002755117 4.693851e-13 1.213808e-11 5 138381516 138381590 75 + 2.131 1.940 -0.639
ENSG00000120733 E011 1.6909512 0.0080315694 7.236635e-01 8.174288e-01 5 138381591 138381748 158 + 0.373 0.470 0.523
ENSG00000120733 E012 149.5192638 0.0002348208 1.419052e-15 5.257875e-14 5 138386022 138386190 169 + 2.251 2.072 -0.601
ENSG00000120733 E013 105.3926762 0.0123827584 6.589775e-03 2.015943e-02 5 138386191 138386288 98 + 2.074 1.965 -0.365
ENSG00000120733 E014 175.7938068 0.0039100792 3.229075e-10 5.228088e-09 5 138386289 138386576 288 + 2.326 2.128 -0.664
ENSG00000120733 E015 54.2092673 0.0050828666 9.086476e-05 4.791398e-04 5 138386577 138386621 45 + 1.812 1.635 -0.598
ENSG00000120733 E016 551.1105393 0.0054140613 5.478834e-06 3.864867e-05 5 138391013 138392261 1249 + 2.787 2.678 -0.360
ENSG00000120733 E017 180.3814248 0.0088520739 1.486309e-02 4.018049e-02 5 138393171 138393372 202 + 2.286 2.220 -0.221
ENSG00000120733 E018 0.5181333 0.0207098381 7.086408e-01 8.062523e-01 5 138397878 138398177 300 + 0.188 0.143 -0.476
ENSG00000120733 E019 193.4862705 0.0005535969 2.107492e-06 1.631156e-05 5 138398178 138398346 169 + 2.318 2.246 -0.241
ENSG00000120733 E020 117.1780182 0.0002834832 1.844913e-06 1.446361e-05 5 138398347 138398392 46 + 2.114 2.014 -0.337
ENSG00000120733 E021 202.1945640 0.0002424206 9.078906e-04 3.641462e-03 5 138399860 138400012 153 + 2.319 2.294 -0.084
ENSG00000120733 E022 168.0560546 0.0016231964 2.234700e-01 3.545306e-01 5 138415132 138415239 108 + 2.220 2.238 0.063
ENSG00000120733 E023 174.1869347 0.0025386004 2.693382e-01 4.078566e-01 5 138417483 138417610 128 + 2.232 2.251 0.064
ENSG00000120733 E024 0.0000000       5 138417808 138417987 180 +      
ENSG00000120733 E025 216.0390664 0.0002128349 3.914273e-01 5.353436e-01 5 138418953 138419232 280 + 2.316 2.354 0.127
ENSG00000120733 E026 245.5137524 0.0001796646 1.113007e-01 2.074597e-01 5 138420706 138420962 257 + 2.380 2.403 0.076
ENSG00000120733 E027 149.0301049 0.0002413682 1.186860e-01 2.180653e-01 5 138424075 138424134 60 + 2.168 2.182 0.044
ENSG00000120733 E028 281.6243244 0.0001618676 2.195434e-01 3.498429e-01 5 138424135 138424341 207 + 2.435 2.468 0.110
ENSG00000120733 E029 275.3298281 0.0019735843 2.189806e-01 3.491639e-01 5 138425411 138425582 172 + 2.393 2.487 0.314
ENSG00000120733 E030 7.1483367 0.0023516326 3.749072e-01 5.193446e-01 5 138425583 138425857 275 + 0.935 0.882 -0.198
ENSG00000120733 E031 9.4350059 0.0039555797 3.533407e-01 4.975830e-01 5 138426741 138426974 234 + 1.043 0.998 -0.166
ENSG00000120733 E032 209.4649210 0.0013683521 4.078189e-01 5.514165e-01 5 138426975 138427065 91 + 2.281 2.363 0.275
ENSG00000120733 E033 228.9126371 0.0035093122 1.110357e-01 2.070635e-01 5 138427189 138427319 131 + 2.302 2.418 0.387
ENSG00000120733 E034 257.2615091 0.0002713617 1.767458e-02 4.643519e-02 5 138427967 138428086 120 + 2.355 2.469 0.377
ENSG00000120733 E035 276.2492973 0.0016579105 2.989897e-02 7.176504e-02 5 138429826 138429965 140 + 2.383 2.503 0.398
ENSG00000120733 E036 269.4300904 0.0002202046 3.562285e-07 3.244695e-06 5 138430249 138430425 177 + 2.345 2.516 0.572
ENSG00000120733 E037 240.5013406 0.0002846410 6.480214e-10 9.956668e-09 5 138431425 138431559 135 + 2.276 2.482 0.685
ENSG00000120733 E038 1337.6801962 0.0089187346 1.746978e-14 5.559260e-13 5 138435620 138437028 1409 + 2.923 3.284 1.202