ENSG00000120727

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000265192 ENSG00000120727 HEK293_OSMI2_2hA HEK293_TMG_2hB PAIP2 protein_coding protein_coding 65.14523 40.49543 97.85465 2.666429 2.918205 1.272672 61.822140 36.616395 94.221868 2.8916164 3.2213469 1.3633313 0.9369458 0.90263333 0.9626333 0.06000000 0.002839733 0.001122099 FALSE  
ENST00000510409 ENSG00000120727 HEK293_OSMI2_2hA HEK293_TMG_2hB PAIP2 protein_coding retained_intron 65.14523 40.49543 97.85465 2.666429 2.918205 1.272672 2.858731 3.337579 3.032201 0.1329132 0.5184634 -0.1380024 0.0553250 0.08356667 0.0312000 -0.05236667 0.001122099 0.001122099 FALSE  
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000120727 E001 4.0203472 8.047401e-03 5.899619e-01 7.129375e-01 5 139341587 139341853 267 + 0.718 0.637 -0.342
ENSG00000120727 E002 8.4456902 2.012590e-03 5.518295e-01 6.812579e-01 5 139341854 139341856 3 + 0.932 1.002 0.260
ENSG00000120727 E003 9.9268390 1.685898e-03 8.185898e-01 8.853483e-01 5 139341857 139341861 5 + 1.014 1.041 0.099
ENSG00000120727 E004 50.7752175 4.716842e-04 2.919825e-03 1.002978e-02 5 139341862 139341877 16 + 1.749 1.592 -0.533
ENSG00000120727 E005 177.3758488 1.387409e-03 9.900385e-01 9.978570e-01 5 139341878 139341918 41 + 2.233 2.235 0.008
ENSG00000120727 E006 266.1302602 5.045077e-04 1.562951e-01 2.701906e-01 5 139341919 139341980 62 + 2.422 2.390 -0.107
ENSG00000120727 E007 0.0000000       5 139342442 139342450 9 +      
ENSG00000120727 E008 0.2998086 2.869801e-02 5.818296e-01   5 139342451 139342616 166 + 0.087 0.169 1.097
ENSG00000120727 E009 0.5944058 3.184342e-01 2.974491e-01 4.387943e-01 5 139342617 139343329 713 + 0.087 0.299 2.153
ENSG00000120727 E010 0.2214452 4.068195e-02 2.265333e-01   5 139343330 139343406 77 + 0.000 0.170 9.573
ENSG00000120727 E011 651.7859724 1.308052e-04 4.530541e-01 5.938295e-01 5 139363759 139363922 164 + 2.801 2.793 -0.026
ENSG00000120727 E012 2.0959762 3.562026e-01 1.122302e-01 2.088020e-01 5 139363923 139364042 120 + 0.324 0.674 1.746
ENSG00000120727 E013 901.1894126 8.482414e-05 9.435680e-01 9.685949e-01 5 139364564 139364743 180 + 2.939 2.941 0.007
ENSG00000120727 E014 26.9685524 8.426216e-04 2.616295e-10 4.293938e-09 5 139364744 139365090 347 + 1.225 1.652 1.473
ENSG00000120727 E015 1514.7591047 9.080340e-04 6.501880e-01 7.612724e-01 5 139368733 139369720 988 + 3.162 3.168 0.022