ENSG00000120333

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000367677 ENSG00000120333 HEK293_OSMI2_2hA HEK293_TMG_2hB MRPS14 protein_coding nonsense_mediated_decay 45.14346 66.35509 33.79393 12.098 1.39402 -0.9732337 27.36146 48.51174 14.82804 10.11501 1.0617668 -1.7093310 0.5725333 0.7223000 0.4377 -0.2846000 1.842584e-10 5.651951e-15 FALSE FALSE
ENST00000476371 ENSG00000120333 HEK293_OSMI2_2hA HEK293_TMG_2hB MRPS14 protein_coding protein_coding 45.14346 66.35509 33.79393 12.098 1.39402 -0.9732337 17.13019 16.90369 18.49927 2.15859 0.4018495 0.1300566 0.4119250 0.2613333 0.5483 0.2869667 5.651951e-15 5.651951e-15 FALSE FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000120333 E001 9.879484 0.0516126432 6.324396e-01 7.472949e-01 1 175010789 175011096 308 - 1.083 0.943 -0.517
ENSG00000120333 E002 28.242139 0.0172145625 2.027053e-02 5.205415e-02 1 175011673 175011751 79 - 1.598 1.334 -0.911
ENSG00000120333 E003 273.048703 0.0274299114 6.252905e-13 1.586014e-11 1 175012958 175014244 1287 - 2.745 2.106 -2.131
ENSG00000120333 E004 606.656686 0.0020864482 1.119864e-03 4.370002e-03 1 175014245 175014672 428 - 2.726 2.744 0.059
ENSG00000120333 E005 944.697320 0.0001607743 6.485345e-18 3.249482e-16 1 175014673 175014851 179 - 2.904 2.948 0.145
ENSG00000120333 E006 808.350962 0.0006149667 5.691116e-04 2.418278e-03 1 175018418 175018576 159 - 2.870 2.863 -0.025
ENSG00000120333 E007 20.871657 0.0009984000 6.541914e-01 7.644583e-01 1 175023272 175023363 92 - 1.308 1.293 -0.053
ENSG00000120333 E008 341.890405 0.0023858676 3.670659e-01 5.114330e-01 1 175023364 175023425 62 - 2.515 2.480 -0.114