ENSG00000120314

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000358337 ENSG00000120314 HEK293_OSMI2_2hA HEK293_TMG_2hB WDR55 protein_coding protein_coding 18.62531 26.85509 13.85857 0.5748122 0.3869897 -0.9539132 10.5178161 16.3445340 7.37290761 0.32686123 0.03070107 -1.14742964 0.55497917 0.60873333 0.532800000 -0.07593333 0.067467404 0.007473525 FALSE  
ENST00000506393 ENSG00000120314 HEK293_OSMI2_2hA HEK293_TMG_2hB WDR55 protein_coding nonsense_mediated_decay 18.62531 26.85509 13.85857 0.5748122 0.3869897 -0.9539132 3.3225833 3.2942648 2.68696610 0.18159191 0.15220614 -0.29299193 0.18951667 0.12296667 0.193566667 0.07060000 0.007473525 0.007473525 FALSE  
ENST00000511232 ENSG00000120314 HEK293_OSMI2_2hA HEK293_TMG_2hB WDR55 protein_coding retained_intron 18.62531 26.85509 13.85857 0.5748122 0.3869897 -0.9539132 0.9741904 0.8422866 0.88728490 0.05613367 0.07519357 0.07422757 0.05530833 0.03126667 0.064200000 0.03293333 0.010622551 0.007473525 FALSE  
MSTRG.27056.1 ENSG00000120314 HEK293_OSMI2_2hA HEK293_TMG_2hB WDR55 protein_coding   18.62531 26.85509 13.85857 0.5748122 0.3869897 -0.9539132 0.9034622 2.4476295 0.09700099 0.75809921 0.09700099 -4.52157136 0.04253333 0.09010000 0.006633333 -0.08346667 0.036133050 0.007473525 TRUE  
MSTRG.27056.2 ENSG00000120314 HEK293_OSMI2_2hA HEK293_TMG_2hB WDR55 protein_coding   18.62531 26.85509 13.85857 0.5748122 0.3869897 -0.9539132 2.9072574 3.9263753 2.81441459 0.47627406 0.16210618 -0.47891590 0.15765833 0.14690000 0.202766667 0.05586667 0.253389567 0.007473525 TRUE  
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000120314 E001 1.646955 0.0920177528 1.944117e-01 3.192983e-01 5 140664705 140664814 110 + 0.170 0.444 1.891
ENSG00000120314 E002 18.378096 0.0198316270 1.104858e-01 2.062412e-01 5 140664815 140664867 53 + 1.346 1.184 -0.569
ENSG00000120314 E003 42.799995 0.0005004800 6.680347e-02 1.380679e-01 5 140664868 140664903 36 + 1.660 1.562 -0.333
ENSG00000120314 E004 173.600791 0.0020373395 1.379646e-03 5.239867e-03 5 140664904 140665103 200 + 2.265 2.161 -0.349
ENSG00000120314 E005 50.262467 0.0008572513 2.008897e-10 3.364982e-09 5 140666575 140668233 1659 + 1.864 1.546 -1.077
ENSG00000120314 E006 155.746201 0.0002744861 4.618090e-01 6.017203e-01 5 140668234 140668334 101 + 2.158 2.141 -0.056
ENSG00000120314 E007 30.382631 0.0006885684 1.289496e-03 4.941163e-03 5 140668335 140668414 80 + 1.585 1.383 -0.696
ENSG00000120314 E008 156.380839 0.0010857478 1.760832e-01 2.961193e-01 5 140668415 140668502 88 + 2.177 2.139 -0.129
ENSG00000120314 E009 217.470185 0.0002015071 3.468114e-02 8.103901e-02 5 140668612 140668791 180 + 2.324 2.276 -0.160
ENSG00000120314 E010 107.410915 0.0002998112 1.879574e-01 3.111442e-01 5 140668891 140668920 30 + 2.016 1.974 -0.142
ENSG00000120314 E011 141.741397 0.0003311529 1.485155e-01 2.596552e-01 5 140668921 140668990 70 + 2.131 2.090 -0.137
ENSG00000120314 E012 33.419445 0.0041411511 5.963504e-03 1.851107e-02 5 140668991 140669078 88 + 1.612 1.431 -0.618
ENSG00000120314 E013 219.715868 0.0008386784 2.009722e-01 3.273879e-01 5 140669079 140669248 170 + 2.314 2.284 -0.101
ENSG00000120314 E014 362.563871 0.0002330991 1.518826e-01 2.642335e-01 5 140669333 140669690 358 + 2.526 2.504 -0.076
ENSG00000120314 E015 645.142802 0.0001518827 7.828080e-07 6.652042e-06 5 140669691 140671268 1578 + 2.701 2.780 0.263
ENSG00000120314 E016 63.522070 0.0009117058 2.633153e-01 4.011862e-01 5 140671269 140671321 53 + 1.712 1.774 0.211
ENSG00000120314 E017 120.489416 0.0042218146 2.069283e-01 3.348705e-01 5 140671322 140671530 209 + 1.985 2.053 0.226
ENSG00000120314 E018 286.831242 0.0022637637 8.372961e-04 3.392502e-03 5 140671531 140672366 836 + 2.326 2.439 0.374
ENSG00000120314 E019 250.572091 0.0033018678 3.875473e-04 1.726765e-03 5 140672378 140674124 1747 + 2.247 2.383 0.455