ENSG00000120053

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000370508 ENSG00000120053 HEK293_OSMI2_2hA HEK293_TMG_2hB GOT1 protein_coding protein_coding 58.07179 81.15695 41.80811 8.12043 0.6565564 -0.9567645 55.189217 78.978944 37.862269 8.2747003 0.21258263 -1.060509 0.94506667 0.97213333 0.9059333 -0.06620000 0.0037285942 0.0001180205 FALSE  
MSTRG.4467.2 ENSG00000120053 HEK293_OSMI2_2hA HEK293_TMG_2hB GOT1 protein_coding   58.07179 81.15695 41.80811 8.12043 0.6565564 -0.9567645 1.691106 1.257705 2.890307 0.2971235 0.09363128 1.193987 0.03294583 0.01603333 0.0691000 0.05306667 0.0001180205 0.0001180205 TRUE  
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000120053 E001 851.237581 0.0011384325 3.727161e-06 2.733112e-05 10 99396870 99397464 595 - 2.805 2.902 0.324
ENSG00000120053 E002 935.108098 0.0021133499 3.520187e-02 8.202713e-02 10 99397465 99397686 222 - 2.878 2.931 0.175
ENSG00000120053 E003 758.150212 0.0004808377 6.029349e-01 7.238038e-01 10 99402580 99402722 143 - 2.818 2.833 0.049
ENSG00000120053 E004 695.688066 0.0004003788 4.296790e-01 5.723706e-01 10 99403469 99403634 166 - 2.797 2.792 -0.018
ENSG00000120053 E005 4.665325 0.0629132734 1.427147e-02 3.884337e-02 10 99403635 99403723 89 - 0.975 0.551 -1.725
ENSG00000120053 E006 604.367608 0.0002666232 3.291586e-01 4.726643e-01 10 99403724 99403874 151 - 2.738 2.729 -0.031
ENSG00000120053 E007 460.899603 0.0004055877 1.289773e-01 2.327459e-01 10 99405756 99405860 105 - 2.630 2.607 -0.079
ENSG00000120053 E008 481.921088 0.0010535304 8.659538e-01 9.176778e-01 10 99406137 99406249 113 - 2.631 2.630 -0.003
ENSG00000120053 E009 544.019982 0.0003895867 2.614332e-01 3.990793e-01 10 99406726 99406849 124 - 2.695 2.680 -0.049
ENSG00000120053 E010 1.843422 0.0088054308 8.740069e-01 9.230211e-01 10 99420267 99420623 357 - 0.397 0.431 0.185
ENSG00000120053 E011 647.255507 0.0012419687 2.511042e-02 6.210143e-02 10 99420624 99420805 182 - 2.791 2.746 -0.151
ENSG00000120053 E012 502.228584 0.0016860047 3.895117e-04 1.734091e-03 10 99430448 99430714 267 - 2.710 2.624 -0.287