ENSG00000119718

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000266126 ENSG00000119718 HEK293_OSMI2_2hA HEK293_TMG_2hB EIF2B2 protein_coding protein_coding 53.38171 82.54184 41.34166 11.06845 1.297335 -0.9973552 14.10695 24.24731 9.118795 3.822568 0.2187393 -1.4099233 0.2555708 0.2921667 0.2211000 -0.07106667 0.0324842318 0.0001673001 FALSE TRUE
ENST00000553401 ENSG00000119718 HEK293_OSMI2_2hA HEK293_TMG_2hB EIF2B2 protein_coding nonsense_mediated_decay 53.38171 82.54184 41.34166 11.06845 1.297335 -0.9973552 11.40290 12.10438 10.273645 1.341163 0.5898029 -0.2363689 0.2220458 0.1478667 0.2491667 0.10130000 0.0001673001 0.0001673001 TRUE TRUE
ENST00000555522 ENSG00000119718 HEK293_OSMI2_2hA HEK293_TMG_2hB EIF2B2 protein_coding retained_intron 53.38171 82.54184 41.34166 11.06845 1.297335 -0.9973552 13.33828 19.97688 11.519983 3.473269 0.4816376 -0.7936626 0.2597000 0.2454000 0.2786667 0.03326667 0.7287118879 0.0001673001 FALSE TRUE
ENST00000556028 ENSG00000119718 HEK293_OSMI2_2hA HEK293_TMG_2hB EIF2B2 protein_coding nonsense_mediated_decay 53.38171 82.54184 41.34166 11.06845 1.297335 -0.9973552 12.99724 25.09567 8.732933 4.630091 1.6180561 -1.5218238 0.2301708 0.3009000 0.2096667 -0.09123333 0.2476569559 0.0001673001 TRUE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000119718 E001 24.076963 0.0039335482 9.032202e-01 9.424200e-01 14 75002921 75002932 12 + 1.338 1.339 0.001
ENSG00000119718 E002 225.052492 0.0030567570 6.101461e-01 7.294946e-01 14 75002933 75002971 39 + 2.269 2.292 0.075
ENSG00000119718 E003 226.385141 0.0027011652 5.394424e-01 6.706990e-01 14 75002972 75002973 2 + 2.269 2.296 0.088
ENSG00000119718 E004 295.890682 0.0019446909 6.586000e-01 7.676771e-01 14 75002974 75003017 44 + 2.392 2.410 0.059
ENSG00000119718 E005 506.451500 0.0012023887 2.393918e-01 3.734186e-01 14 75003018 75003153 136 + 2.656 2.633 -0.079
ENSG00000119718 E006 15.834481 0.0236586051 2.720456e-05 1.634889e-04 14 75003154 75003274 121 + 1.458 0.992 -1.654
ENSG00000119718 E007 569.876792 0.0019627481 3.168189e-01 4.596200e-01 14 75003275 75003395 121 + 2.707 2.683 -0.080
ENSG00000119718 E008 11.276082 0.0229349567 1.040039e-03 4.097667e-03 14 75003396 75003550 155 + 1.282 0.892 -1.416
ENSG00000119718 E009 664.385622 0.0021248273 5.613945e-02 1.199482e-01 14 75003551 75003699 149 + 2.790 2.744 -0.151
ENSG00000119718 E010 733.703602 0.0002268516 2.223647e-01 3.532203e-01 14 75004737 75004900 164 + 2.810 2.801 -0.030
ENSG00000119718 E011 8.403872 0.0024804315 4.392429e-07 3.928841e-06 14 75004901 75005102 202 + 1.257 0.714 -2.032
ENSG00000119718 E012 4.294031 0.0078867134 1.008174e-05 6.692909e-05 14 75005841 75005865 25 + 1.046 0.445 -2.505
ENSG00000119718 E013 382.995581 0.0001321078 3.577492e-01 5.021673e-01 14 75005866 75005896 31 + 2.528 2.521 -0.024
ENSG00000119718 E014 283.197431 0.0001606270 6.140145e-02 1.289900e-01 14 75005897 75005899 3 + 2.416 2.383 -0.109
ENSG00000119718 E015 277.768548 0.0001962579 5.401210e-01 6.713092e-01 14 75005900 75005904 5 + 2.386 2.383 -0.011
ENSG00000119718 E016 449.718704 0.0001471545 8.269787e-01 8.911652e-01 14 75005905 75005961 57 + 2.581 2.597 0.053
ENSG00000119718 E017 4.349196 0.0040789795 6.278267e-03 1.934234e-02 14 75006304 75006576 273 + 0.927 0.554 -1.531
ENSG00000119718 E018 696.315152 0.0009139872 1.385606e-01 2.461059e-01 14 75006577 75006714 138 + 2.749 2.788 0.127
ENSG00000119718 E019 2.929852 0.1340187996 1.285603e-01 2.321615e-01 14 75007061 75007416 356 + 0.764 0.465 -1.327
ENSG00000119718 E020 539.972186 0.0025795614 5.186665e-01 6.528935e-01 14 75007722 75007788 67 + 2.647 2.673 0.085
ENSG00000119718 E021 1267.220817 0.0018730610 1.002193e-03 3.965961e-03 14 75009031 75012366 3336 + 2.977 3.051 0.248