ENSG00000119541

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000238497 ENSG00000119541 HEK293_OSMI2_2hA HEK293_TMG_2hB VPS4B protein_coding protein_coding 8.323484 2.757051 13.8956 0.4042316 1.411057 2.329245 7.2287651 2.725862 11.577170 0.3758958 0.4240051 2.082461 0.91214583 0.9910667 0.84466667 -0.14640000 0.02852416 0.0003848069 FALSE TRUE
ENST00000591519 ENSG00000119541 HEK293_OSMI2_2hA HEK293_TMG_2hB VPS4B protein_coding protein_coding 8.323484 2.757051 13.8956 0.4042316 1.411057 2.329245 0.8215087 0.000000 1.449239 0.0000000 0.8086384 7.189072 0.05620417 0.0000000 0.09413333 0.09413333 0.38083521 0.0003848069 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000119541 E001 268.0333175 0.0056527135 3.793821e-15 1.329746e-13 18 63389190 63390862 1673 - 2.246 2.566 1.066
ENSG00000119541 E002 77.6837750 0.0022903880 9.788998e-01 9.908456e-01 18 63390863 63391076 214 - 1.784 1.840 0.187
ENSG00000119541 E003 38.0053043 0.0005723010 4.621606e-01 6.020418e-01 18 63393409 63393443 35 - 1.489 1.493 0.012
ENSG00000119541 E004 51.7536990 0.0006040896 9.250316e-01 9.567940e-01 18 63393444 63393549 106 - 1.611 1.661 0.170
ENSG00000119541 E005 1.0319011 0.0120971397 7.962973e-01 8.699691e-01 18 63393550 63393566 17 - 0.271 0.239 -0.238
ENSG00000119541 E006 2.3669347 0.0062452175 2.194330e-02 5.555651e-02 18 63396342 63396589 248 - 0.520 0.000 -13.081
ENSG00000119541 E007 1.6263095 0.0085201844 7.140894e-02 1.456729e-01 18 63396590 63397033 444 - 0.413 0.000 -12.541
ENSG00000119541 E008 73.7567026 0.0061235041 6.964890e-01 7.968244e-01 18 63397034 63397253 220 - 1.765 1.793 0.091
ENSG00000119541 E009 29.1799734 0.0141189209 6.136508e-01 7.323013e-01 18 63399242 63399254 13 - 1.377 1.378 0.004
ENSG00000119541 E010 51.8411544 0.0006245351 3.032912e-02 7.261625e-02 18 63399255 63399323 69 - 1.633 1.550 -0.282
ENSG00000119541 E011 73.0528212 0.0026377952 3.930046e-04 1.747982e-03 18 63400048 63400196 149 - 1.793 1.632 -0.543
ENSG00000119541 E012 74.6345466 0.0017120218 4.199758e-05 2.408523e-04 18 63400547 63400703 157 - 1.807 1.624 -0.620
ENSG00000119541 E013 60.0791688 0.0003861298 7.003832e-03 2.123358e-02 18 63403707 63403826 120 - 1.704 1.601 -0.351
ENSG00000119541 E014 0.1451727 0.0434503029 1.000000e+00   18 63404509 63404714 206 - 0.059 0.000 -9.081
ENSG00000119541 E015 0.3299976 0.0274424043 1.615864e-02   18 63407376 63407431 56 - 0.000 0.392 14.499
ENSG00000119541 E016 49.7674702 0.0004809316 1.987405e-02 5.122283e-02 18 63407432 63407499 68 - 1.626 1.532 -0.321
ENSG00000119541 E017 59.9428315 0.0021698338 6.926196e-03 2.103119e-02 18 63410290 63410446 157 - 1.707 1.592 -0.389
ENSG00000119541 E018 56.5896079 0.0053632562 2.703485e-03 9.384630e-03 18 63411467 63411578 112 - 1.687 1.522 -0.561
ENSG00000119541 E019 57.5003523 0.0019831755 6.876111e-02 1.413153e-01 18 63422233 63422483 251 - 1.676 1.617 -0.202