ENSG00000119523

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000238477 ENSG00000119523 HEK293_OSMI2_2hA HEK293_TMG_2hB ALG2 protein_coding nonsense_mediated_decay 20.91278 17.03578 26.91926 2.321284 0.3384382 0.6597597 1.219132 1.375589 1.53495 0.1297016 0.0970312 0.1570607 0.05998333 0.0817 0.05696667 -0.02473333 1.630278e-01 5.882982e-05 FALSE TRUE
ENST00000319033 ENSG00000119523 HEK293_OSMI2_2hA HEK293_TMG_2hB ALG2 protein_coding protein_coding 20.91278 17.03578 26.91926 2.321284 0.3384382 0.6597597 14.003502 11.977904 16.36107 1.7710653 0.4394043 0.4495691 0.67582917 0.7011 0.60760000 -0.09350000 3.606472e-03 5.882982e-05 FALSE TRUE
ENST00000476832 ENSG00000119523 HEK293_OSMI2_2hA HEK293_TMG_2hB ALG2 protein_coding protein_coding 20.91278 17.03578 26.91926 2.321284 0.3384382 0.6597597 5.690147 3.682290 9.02324 0.4346487 0.1933671 1.2907278 0.26419583 0.2172 0.33543333 0.11823333 5.882982e-05 5.882982e-05 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000119523 E001 0.1451727 0.0435531985 0.6922347430   9 99216425 99216425 1 - 0.106 0.000 -8.931
ENSG00000119523 E002 101.2328099 0.0124999383 0.0871715804 0.170857416 9 99216426 99217371 946 - 2.058 1.957 -0.338
ENSG00000119523 E003 905.9168361 0.0002290620 0.0007079308 0.002927516 9 99217372 99218836 1465 - 2.957 2.968 0.035
ENSG00000119523 E004 48.6951685 0.0005166672 0.1922350628 0.316557486 9 99220979 99221137 159 - 1.672 1.727 0.188
ENSG00000119523 E005 7.4522969 0.0022146814 0.8886310227 0.932802596 9 99221138 99221140 3 - 0.928 0.937 0.032
ENSG00000119523 E006 156.1191891 0.0101852652 0.1363632417 0.242986607 9 99221547 99221942 396 - 2.241 2.138 -0.344