ENSG00000119446

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000417201 ENSG00000119446 HEK293_OSMI2_2hA HEK293_TMG_2hB RBM18 protein_coding protein_coding 19.54024 15.16522 28.64029 0.8299496 0.4418069 0.9168323 5.091368 2.110251 8.907810 0.3334554 0.3044653 2.0724542 0.2363500 0.1381667 0.3108333 0.17266667 3.560838e-07 3.560838e-07 FALSE TRUE
MSTRG.33328.2 ENSG00000119446 HEK293_OSMI2_2hA HEK293_TMG_2hB RBM18 protein_coding   19.54024 15.16522 28.64029 0.8299496 0.4418069 0.9168323 5.532002 5.267383 9.129286 0.6976361 1.1060748 0.7922589 0.2913833 0.3506667 0.3197333 -0.03093333 8.914915e-01 3.560838e-07 FALSE TRUE
MSTRG.33328.3 ENSG00000119446 HEK293_OSMI2_2hA HEK293_TMG_2hB RBM18 protein_coding   19.54024 15.16522 28.64029 0.8299496 0.4418069 0.9168323 8.396090 7.480624 9.965368 0.9031508 1.1785361 0.4132840 0.4471875 0.4901667 0.3472667 -0.14290000 1.107657e-01 3.560838e-07 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000119446 E001 514.604365 0.0099336350 4.572376e-08 4.970865e-07 9 122237622 122241647 4026 - 2.784 2.519 -0.882
ENSG00000119446 E002 34.361444 0.0022565147 6.970761e-06 4.803018e-05 9 122241648 122241648 1 - 1.646 1.273 -1.284
ENSG00000119446 E003 44.013823 0.0027386096 1.779756e-05 1.116492e-04 9 122241649 122241669 21 - 1.742 1.415 -1.115
ENSG00000119446 E004 77.458853 0.0035993664 6.467422e-01 7.585553e-01 9 122241670 122241757 88 - 1.913 1.841 -0.244
ENSG00000119446 E005 248.563696 0.0006336603 6.410370e-07 5.548868e-06 9 122241758 122242043 286 - 2.361 2.428 0.222
ENSG00000119446 E006 3.259070 0.0409614171 9.430092e-01 9.682622e-01 9 122243744 122243868 125 - 0.629 0.574 -0.243
ENSG00000119446 E007 121.640934 0.0010202598 3.109902e-07 2.867664e-06 9 122245256 122245341 86 - 2.028 2.152 0.417
ENSG00000119446 E008 124.916860 0.0009302001 9.070569e-06 6.087329e-05 9 122247518 122247604 87 - 2.052 2.151 0.329
ENSG00000119446 E009 119.590213 0.0075182309 7.684588e-03 2.298732e-02 9 122251847 122251936 90 - 2.040 2.119 0.264
ENSG00000119446 E010 104.738948 0.0012764784 2.854341e-02 6.910231e-02 9 122251937 122251973 37 - 2.001 2.032 0.102
ENSG00000119446 E011 0.628762 0.0172671820 4.407817e-01 5.824425e-01 9 122254274 122254367 94 - 0.166 0.281 0.969
ENSG00000119446 E012 162.441202 0.0105845300 1.494524e-01 2.609257e-01 9 122261380 122261498 119 - 2.194 2.213 0.065
ENSG00000119446 E013 75.115417 0.0153090038 5.760243e-01 7.015318e-01 9 122261499 122261508 10 - 1.876 1.857 -0.063
ENSG00000119446 E014 107.818747 0.0126035076 3.273639e-01 4.708078e-01 9 122264715 122264840 126 - 2.023 2.026 0.010