ENSG00000119396

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000373840 ENSG00000119396 HEK293_OSMI2_2hA HEK293_TMG_2hB RAB14 protein_coding protein_coding 26.23958 17.98163 37.11092 2.318073 0.8052737 1.044906 23.498147 17.66038 31.377321 2.431461 0.8837526 0.8288487 0.92065833 0.9802667 0.8453000 -0.1349667 4.147125e-03 1.516792e-35 FALSE TRUE
ENST00000451303 ENSG00000119396 HEK293_OSMI2_2hA HEK293_TMG_2hB RAB14 protein_coding protein_coding 26.23958 17.98163 37.11092 2.318073 0.8052737 1.044906 2.112563 0.00000 5.376591 0.000000 0.2107990 9.0732287 0.05689583 0.0000000 0.1451667 0.1451667 1.516792e-35 1.516792e-35 FALSE FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000119396 E001 2177.930092 0.0030272766 3.668558e-08 4.059887e-07 9 121178133 121181498 3366 - 3.269 3.406 0.453
ENSG00000119396 E002 271.973558 0.0011272523 3.559782e-03 1.189694e-02 9 121181499 121181573 75 - 2.429 2.420 -0.028
ENSG00000119396 E003 6.489374 0.0060897992 3.715807e-01 5.160421e-01 9 121182916 121182929 14 - 0.889 0.840 -0.189
ENSG00000119396 E004 201.387195 0.0002509374 5.977668e-03 1.854896e-02 9 121182930 121182960 31 - 2.295 2.297 0.006
ENSG00000119396 E005 225.826603 0.0018718344 9.675889e-04 3.847508e-03 9 121183311 121183398 88 - 2.358 2.322 -0.122
ENSG00000119396 E006 199.173854 0.0002531766 3.029716e-07 2.801359e-06 9 121186953 121187016 64 - 2.315 2.254 -0.205
ENSG00000119396 E007 117.739116 0.0023069020 1.136262e-03 4.425059e-03 9 121187017 121187019 3 - 2.088 2.025 -0.211
ENSG00000119396 E008 273.673318 0.0013245597 4.836214e-06 3.455817e-05 9 121190554 121190731 178 - 2.450 2.395 -0.182
ENSG00000119396 E009 130.008465 0.0037118436 4.644695e-02 1.028417e-01 9 121192171 121192224 54 - 2.113 2.096 -0.057
ENSG00000119396 E010 85.247251 0.0010055032 1.225824e-01 2.236323e-01 9 121193361 121193419 59 - 1.921 1.928 0.026
ENSG00000119396 E011 111.175111 0.0004653795 3.078519e-03 1.049686e-02 9 121201639 121201917 279 - 2.052 2.020 -0.105
ENSG00000119396 E012 0.000000       9 121222200 121222253 54 -      
ENSG00000119396 E013 0.000000       9 121222910 121223014 105 -