ENSG00000118960

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000304031 ENSG00000118960 HEK293_OSMI2_2hA HEK293_TMG_2hB HS1BP3 protein_coding protein_coding 27.08045 48.99511 15.19543 1.505363 0.6982472 -1.688345 17.806979 34.375048 8.084897 1.048342 0.54393225 -2.0866968 0.6173917 0.7016667 0.5311 -0.1705667 1.365692e-09 1.365692e-09 FALSE TRUE
MSTRG.18020.5 ENSG00000118960 HEK293_OSMI2_2hA HEK293_TMG_2hB HS1BP3 protein_coding   27.08045 48.99511 15.19543 1.505363 0.6982472 -1.688345 6.154743 7.851757 5.588144 2.248879 0.06893953 -0.4899031 0.2683792 0.1578000 0.3689 0.2111000 3.149362e-02 1.365692e-09 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000118960 E001 0.0000000       2 20560448 20560515 68 -      
ENSG00000118960 E002 0.1515154 0.0427119839 1.146509e-01   2 20592617 20592794 178 - 0.212 0.000 -10.474
ENSG00000118960 E003 0.0000000       2 20598208 20598265 58 -      
ENSG00000118960 E004 20.9926484 0.0961430579 5.692986e-02 1.213387e-01 2 20612297 20617817 5521 - 1.494 1.153 -1.189
ENSG00000118960 E005 940.8380410 0.0015050401 6.445114e-10 9.909971e-09 2 20617818 20619245 1428 - 2.754 2.880 0.417
ENSG00000118960 E006 1.0403321 0.0779644626 4.671725e-02 1.033230e-01 2 20622179 20622187 9 - 0.547 0.175 -2.344
ENSG00000118960 E007 38.4658890 0.0005956196 1.411726e-01 2.496427e-01 2 20622188 20622319 132 - 1.560 1.468 -0.316
ENSG00000118960 E008 72.5758019 0.0024068388 1.748725e-02 4.601642e-02 2 20622320 20623895 1576 - 1.623 1.773 0.508
ENSG00000118960 E009 275.7190087 0.0002241348 9.636242e-01 9.810657e-01 2 20623896 20624031 136 - 2.320 2.328 0.025
ENSG00000118960 E010 275.0908122 0.0009447676 1.792469e-01 3.001767e-01 2 20624732 20624892 161 - 2.286 2.334 0.159
ENSG00000118960 E011 25.1079443 0.0008191594 9.910664e-01 9.985387e-01 2 20634716 20638435 3720 - 1.299 1.304 0.017
ENSG00000118960 E012 359.5006282 0.0001745712 1.879040e-01 3.110925e-01 2 20638436 20638652 217 - 2.409 2.446 0.123
ENSG00000118960 E013 25.3311766 0.0008160230 6.034845e-01 7.242801e-01 2 20640025 20640972 948 - 1.271 1.322 0.178
ENSG00000118960 E014 325.2497087 0.0013948020 9.042452e-03 2.638408e-02 2 20640973 20641134 162 - 2.449 2.384 -0.217
ENSG00000118960 E015 191.0633516 0.0057451449 1.367578e-03 5.200913e-03 2 20641135 20641180 46 - 2.278 2.139 -0.464
ENSG00000118960 E016 257.2831550 0.0003963491 2.112161e-08 2.451404e-07 2 20645340 20645505 166 - 2.406 2.265 -0.469
ENSG00000118960 E017 118.8695479 0.0041066966 2.300473e-03 8.152069e-03 2 20651032 20651140 109 - 2.071 1.932 -0.465