ENSG00000118922

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000377669 ENSG00000118922 HEK293_OSMI2_2hA HEK293_TMG_2hB KLF12 protein_coding protein_coding 4.147804 2.154005 6.97216 0.05388628 0.2024419 1.68997 2.5112823 0.9403176 4.4217260 0.0547013 0.06678099 2.2213871 0.56415833 0.4372667 0.6347667 0.1975 0.004261221 0.004261221 FALSE TRUE
MSTRG.8871.2 ENSG00000118922 HEK293_OSMI2_2hA HEK293_TMG_2hB KLF12 protein_coding   4.147804 2.154005 6.97216 0.05388628 0.2024419 1.68997 1.3340447 0.8280942 2.0340140 0.1154268 0.16559086 1.2862208 0.36312917 0.3860333 0.2921333 -0.0939 0.486702335 0.004261221 FALSE TRUE
MSTRG.8871.3 ENSG00000118922 HEK293_OSMI2_2hA HEK293_TMG_2hB KLF12 protein_coding   4.147804 2.154005 6.97216 0.05388628 0.2024419 1.68997 0.2961162 0.3666123 0.5164196 0.1085557 0.19306877 0.4831332 0.07094583 0.1680000 0.0731000 -0.0949 0.295852744 0.004261221 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000118922 E001 615.0547258 0.0003996583 9.290251e-13 2.293173e-11 13 73686089 73695232 9144 - 2.756 2.678 -0.259
ENSG00000118922 E002 25.3935939 0.0017892624 6.398670e-02 1.333302e-01 13 73695233 73695301 69 - 1.324 1.463 0.479
ENSG00000118922 E003 58.7031068 0.0008926628 9.552886e-03 2.766549e-02 13 73695302 73695671 370 - 1.682 1.811 0.436
ENSG00000118922 E004 35.7608676 0.0006122009 1.403169e-04 7.049221e-04 13 73715368 73715525 158 - 1.439 1.671 0.791
ENSG00000118922 E005 23.3035767 0.0031505358 2.347970e-03 8.300662e-03 13 73764938 73765000 63 - 1.261 1.496 0.815
ENSG00000118922 E006 0.1482932 0.0410855686 1.341105e-01   13 73812873 73813151 279 - 0.000 0.203 12.637
ENSG00000118922 E007 39.8883388 0.0016638453 8.614532e-05 4.569629e-04 13 73813152 73813287 136 - 1.485 1.718 0.795
ENSG00000118922 E008 79.4361788 0.0060501236 4.255086e-02 9.572750e-02 13 73845827 73846373 547 - 1.821 1.927 0.357
ENSG00000118922 E009 22.5025841 0.0016099446 3.486957e-01 4.929250e-01 13 73943981 73944070 90 - 1.342 1.262 -0.281
ENSG00000118922 E010 15.5216582 0.0015605194 1.084251e-01 2.032113e-01 13 73994990 73995053 64 - 1.212 1.046 -0.596
ENSG00000118922 E011 0.0000000       13 73995054 73995056 3 -      
ENSG00000118922 E012 4.8826481 0.0532062035 5.775198e-01 7.027703e-01 13 74133739 74133929 191 - 0.706 0.801 0.381
ENSG00000118922 E013 11.7875132 0.0040964598 4.329357e-01 5.753812e-01 13 74133972 74134156 185 - 1.086 0.996 -0.329
ENSG00000118922 E014 0.7416694 0.0181281482 8.973305e-01 9.384299e-01 13 74134157 74134168 12 - 0.233 0.203 -0.258
ENSG00000118922 E015 1.0965559 0.1118317524 2.063357e-04 9.911635e-04 13 74135168 74135247 80 - 0.000 0.662 13.847