ENSG00000118260

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000418081 ENSG00000118260 HEK293_OSMI2_2hA HEK293_TMG_2hB CREB1 protein_coding nonsense_mediated_decay 11.82757 3.325241 18.48411 0.2245306 0.606767 2.471202 0.7813889 0.29678560 1.7831566 0.15346661 0.21767986 2.5471988 0.06602917 0.09070000 0.09636667 0.005666667 0.85957423 0.02252882 TRUE  
ENST00000430624 ENSG00000118260 HEK293_OSMI2_2hA HEK293_TMG_2hB CREB1 protein_coding protein_coding 11.82757 3.325241 18.48411 0.2245306 0.606767 2.471202 0.8723944 0.16789063 1.4399307 0.16789063 0.23527929 3.0269216 0.07066250 0.05313333 0.07890000 0.025766667 0.47667042 0.02252882 FALSE  
ENST00000432329 ENSG00000118260 HEK293_OSMI2_2hA HEK293_TMG_2hB CREB1 protein_coding protein_coding 11.82757 3.325241 18.48411 0.2245306 0.606767 2.471202 1.2371284 0.65057993 2.6130196 0.03297706 0.48919596 1.9894235 0.12211667 0.19623333 0.14150000 -0.054733333 0.43420728 0.02252882 FALSE  
ENST00000445803 ENSG00000118260 HEK293_OSMI2_2hA HEK293_TMG_2hB CREB1 protein_coding protein_coding 11.82757 3.325241 18.48411 0.2245306 0.606767 2.471202 1.1196235 0.00000000 1.7374207 0.00000000 0.99053692 7.4490832 0.08871250 0.00000000 0.09250000 0.092500000 0.40329458 0.02252882 FALSE  
ENST00000448277 ENSG00000118260 HEK293_OSMI2_2hA HEK293_TMG_2hB CREB1 protein_coding protein_coding 11.82757 3.325241 18.48411 0.2245306 0.606767 2.471202 3.3490904 0.89315711 5.3205366 0.45091163 0.31378844 2.5612319 0.30330417 0.26736667 0.28893333 0.021566667 0.88446775 0.02252882 FALSE  
ENST00000455757 ENSG00000118260 HEK293_OSMI2_2hA HEK293_TMG_2hB CREB1 protein_coding protein_coding 11.82757 3.325241 18.48411 0.2245306 0.606767 2.471202 0.3714096 0.30227663 0.3554842 0.12430671 0.07098602 0.2269846 0.05855833 0.09103333 0.01906667 -0.071966667 0.02252882 0.02252882 FALSE  
ENST00000494983 ENSG00000118260 HEK293_OSMI2_2hA HEK293_TMG_2hB CREB1 protein_coding processed_transcript 11.82757 3.325241 18.48411 0.2245306 0.606767 2.471202 1.4523088 0.66518071 1.8219130 0.38546261 0.58165862 1.4400054 0.10041667 0.19033333 0.09810000 -0.092233333 0.93663232 0.02252882 FALSE  
MSTRG.19877.4 ENSG00000118260 HEK293_OSMI2_2hA HEK293_TMG_2hB CREB1 protein_coding   11.82757 3.325241 18.48411 0.2245306 0.606767 2.471202 0.7909486 0.05652826 1.2790053 0.05652826 0.06615093 4.2761472 0.06883750 0.01856667 0.06943333 0.050866667 0.17019967 0.02252882 TRUE  
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000118260 E001 0.0000000       2 207529737 207529891 155 +      
ENSG00000118260 E002 0.4427912 0.2018608234 0.477066775 0.61567245 2 207529892 207529922 31 + 0.111 0.235 1.296
ENSG00000118260 E003 1.3285892 0.0098721489 0.650871365 0.76185127 2 207529923 207529931 9 + 0.336 0.237 -0.689
ENSG00000118260 E004 2.9861318 0.0051362111 0.846990828 0.90495266 2 207529932 207529939 8 + 0.542 0.502 -0.192
ENSG00000118260 E005 9.6804077 0.0406814631 0.483307743 0.62133101 2 207529940 207529961 22 + 0.962 0.832 -0.493
ENSG00000118260 E006 29.5740216 0.0014441144 0.168950597 0.28681623 2 207529962 207530018 57 + 1.365 1.467 0.350
ENSG00000118260 E007 40.1214994 0.0152687723 0.285040805 0.42534185 2 207530019 207530071 53 + 1.496 1.582 0.294
ENSG00000118260 E008 41.1248688 0.0011647104 0.042211767 0.09511475 2 207530072 207530103 32 + 1.499 1.627 0.438
ENSG00000118260 E009 41.5071935 0.0007070376 0.034330845 0.08039676 2 207530104 207530134 31 + 1.503 1.634 0.449
ENSG00000118260 E010 0.0000000       2 207534602 207534683 82 +      
ENSG00000118260 E011 0.0000000       2 207550261 207550595 335 +      
ENSG00000118260 E012 64.4984793 0.0043748393 0.318356654 0.46132445 2 207555628 207555749 122 + 1.709 1.764 0.188
ENSG00000118260 E013 0.0000000       2 207559122 207559170 49 +      
ENSG00000118260 E014 0.0000000       2 207559171 207559187 17 +      
ENSG00000118260 E015 0.0000000       2 207559188 207559225 38 +      
ENSG00000118260 E016 0.0000000       2 207559226 207559255 30 +      
ENSG00000118260 E017 55.6952950 0.0156058529 0.955605407 0.97614365 2 207560226 207560313 88 + 1.658 1.663 0.017
ENSG00000118260 E018 49.3717030 0.0093440174 0.864014560 0.91633273 2 207560314 207560372 59 + 1.610 1.593 -0.060
ENSG00000118260 E019 13.0975401 0.0013835978 0.833030525 0.89538057 2 207561119 207561160 42 + 1.056 1.075 0.068
ENSG00000118260 E020 60.8072881 0.0004546220 0.806453392 0.87698352 2 207567463 207567563 101 + 1.693 1.701 0.028
ENSG00000118260 E021 0.5943067 0.0229884215 0.657066809 0.76650781 2 207567564 207568150 587 + 0.158 0.237 0.725
ENSG00000118260 E022 68.7984859 0.0003499779 0.743230292 0.83193154 2 207570179 207570321 143 + 1.743 1.755 0.039
ENSG00000118260 E023 1.2157810 0.0133330063 0.104709522 0.19768506 2 207571734 207571777 44 + 0.200 0.502 1.900
ENSG00000118260 E024 33.5833158 0.0006544198 0.651205263 0.76212908 2 207575272 207575285 14 + 1.450 1.409 -0.140
ENSG00000118260 E025 78.7652962 0.0004484411 0.480059983 0.61836954 2 207575286 207575454 169 + 1.797 1.826 0.098
ENSG00000118260 E026 2.0776335 0.0066962204 0.298300234 0.43974193 2 207576631 207576635 5 + 0.462 0.237 -1.390
ENSG00000118260 E027 2.5246911 0.0069872278 0.578216003 0.70336904 2 207576636 207576693 58 + 0.504 0.389 -0.597
ENSG00000118260 E028 7.7609105 0.0020927333 0.577244541 0.70252845 2 207577110 207577224 115 + 0.871 0.784 -0.340
ENSG00000118260 E029 80.4524560 0.0004265808 0.096353480 0.18489853 2 207577505 207577655 151 + 1.797 1.871 0.249
ENSG00000118260 E030 3.4416310 0.0046939420 0.007636314 0.02286369 2 207577656 207578065 410 + 0.462 0.876 1.780
ENSG00000118260 E031 72.1584932 0.0039421442 0.895089629 0.93696048 2 207596914 207597164 251 + 1.765 1.766 0.003
ENSG00000118260 E032 49.0629228 0.0086610914 0.742642923 0.83149515 2 207597165 207597326 162 + 1.597 1.613 0.054
ENSG00000118260 E033 26.0038340 0.0067147453 0.976228478 0.98913545 2 207597327 207597347 21 + 1.334 1.329 -0.018
ENSG00000118260 E034 52.1451742 0.0005063580 0.937700398 0.96482491 2 207597348 207597483 136 + 1.630 1.619 -0.036
ENSG00000118260 E035 117.8699600 0.0002524724 0.061174784 0.12860084 2 207597484 207599079 1596 + 1.992 1.907 -0.284
ENSG00000118260 E036 404.8441318 0.0019056048 0.003965592 0.01304650 2 207599080 207605988 6909 + 2.523 2.442 -0.270