ENSG00000117691

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000366988 ENSG00000117691 HEK293_OSMI2_2hA HEK293_TMG_2hB NENF protein_coding protein_coding 232.0878 456.877 112.8098 6.730217 1.739988 -2.017817 172.079397 305.318507 91.718731 21.1395972 1.6533026 -1.734917 0.74830833 0.669233333 0.81296667 0.1437333 4.186624e-02 1.175982e-25 FALSE TRUE
ENST00000479589 ENSG00000117691 HEK293_OSMI2_2hA HEK293_TMG_2hB NENF protein_coding processed_transcript 232.0878 456.877 112.8098 6.730217 1.739988 -2.017817 18.935184 36.898129 7.933854 2.1443151 0.3144463 -2.216028 0.07562083 0.080833333 0.07033333 -0.0105000 5.655912e-01 1.175982e-25 FALSE FALSE
MSTRG.3025.1 ENSG00000117691 HEK293_OSMI2_2hA HEK293_TMG_2hB NENF protein_coding   232.0878 456.877 112.8098 6.730217 1.739988 -2.017817 5.216792 1.747057 8.163958 0.8291337 0.5982287 2.217874 0.02735417 0.003866667 0.07226667 0.0684000 3.920226e-05 1.175982e-25 TRUE FALSE
MSTRG.3025.8 ENSG00000117691 HEK293_OSMI2_2hA HEK293_TMG_2hB NENF protein_coding   232.0878 456.877 112.8098 6.730217 1.739988 -2.017817 31.489451 108.488760 0.000000 28.4840382 0.0000000 -13.405391 0.12772917 0.236333333 0.00000000 -0.2363333 1.175982e-25 1.175982e-25 FALSE FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000117691 E001 8.2084045 0.0132844105 2.296342e-17 1.068357e-15 1 212432854 212432880 27 + 1.457 0.423 -4.066
ENSG00000117691 E002 44.9547760 0.0243511202 2.051069e-32 4.107494e-30 1 212432881 212432919 39 + 2.171 1.032 -3.915
ENSG00000117691 E003 47.9377978 0.0271178338 2.452668e-30 4.227940e-28 1 212432920 212432922 3 + 2.197 1.065 -3.878
ENSG00000117691 E004 150.2685109 0.0630021701 8.003800e-15 2.671887e-13 1 212432923 212432972 50 + 2.648 1.653 -3.335
ENSG00000117691 E005 174.3892363 0.0363600487 9.354653e-16 3.545131e-14 1 212432973 212432990 18 + 2.633 1.846 -2.635
ENSG00000117691 E006 1264.1853726 0.0047198607 2.389328e-04 1.128749e-03 1 212432991 212433120 130 + 3.087 2.956 -0.437
ENSG00000117691 E007 2.3677859 0.0079203392 2.866170e-02 6.933198e-02 1 212434705 212434901 197 + 0.741 0.365 -1.775
ENSG00000117691 E008 1.1759384 0.0119362098 6.370115e-01 7.509547e-01 1 212434902 212435033 132 + 0.359 0.261 -0.644
ENSG00000117691 E009 0.7384326 0.0239153544 8.424102e-01 9.018081e-01 1 212435448 212435491 44 + 0.216 0.175 -0.378
ENSG00000117691 E010 13.3332748 0.0017841205 6.117280e-01 7.308004e-01 1 212442546 212442564 19 + 1.100 1.030 -0.253
ENSG00000117691 E011 854.6368502 0.0045351936 2.983996e-02 7.165062e-02 1 212442565 212442568 4 + 2.882 2.795 -0.287
ENSG00000117691 E012 1684.2076965 0.0008408345 7.764957e-01 8.560729e-01 1 212442569 212442625 57 + 3.120 3.103 -0.057
ENSG00000117691 E013 1380.6866862 0.0001465822 1.005716e-01 1.913259e-01 1 212444339 212444340 2 + 3.015 3.021 0.022
ENSG00000117691 E014 3381.2002752 0.0003039374 1.327021e-13 3.706873e-12 1 212444341 212444442 102 + 3.360 3.419 0.194
ENSG00000117691 E015 4907.8411867 0.0020890061 5.610171e-15 1.917773e-13 1 212445830 212446379 550 + 3.473 3.590 0.388