ENSG00000117650

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000366999 ENSG00000117650 HEK293_OSMI2_2hA HEK293_TMG_2hB NEK2 protein_coding protein_coding 24.62967 10.71839 35.9567 0.847407 1.015912 1.745228 14.973116 8.97295689 19.493049 1.03952069 1.1033232 1.118437 0.6876458 0.832266667 0.5413667 -0.29090000 7.363257e-06 7.782089e-20 FALSE TRUE
ENST00000540251 ENSG00000117650 HEK293_OSMI2_2hA HEK293_TMG_2hB NEK2 protein_coding protein_coding 24.62967 10.71839 35.9567 0.847407 1.015912 1.745228 4.016897 0.06116893 6.811846 0.03673085 0.6529484 6.582771 0.1120917 0.005533333 0.1891333 0.18360000 7.782089e-20 7.782089e-20 FALSE TRUE
MSTRG.3001.3 ENSG00000117650 HEK293_OSMI2_2hA HEK293_TMG_2hB NEK2 protein_coding   24.62967 10.71839 35.9567 0.847407 1.015912 1.745228 4.433853 1.38741108 7.797809 0.28847962 0.2352470 2.482161 0.1466625 0.134233333 0.2175000 0.08326667 2.311879e-01 7.782089e-20 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000117650 E001 3.9275586 0.2038006589 5.877374e-01 7.109935e-01 1 211658657 211658712 56 - 0.582 0.778 0.829
ENSG00000117650 E002 152.9383399 0.0046744900 4.409062e-17 1.978876e-15 1 211662772 211663141 370 - 2.007 2.373 1.223
ENSG00000117650 E003 197.9590816 0.0007659962 9.022145e-14 2.579952e-12 1 211663142 211663402 261 - 2.188 2.404 0.720
ENSG00000117650 E004 146.1575119 0.0002652511 7.321865e-05 3.956488e-04 1 211663403 211663524 122 - 2.093 2.225 0.441
ENSG00000117650 E005 153.9990754 0.0006721751 2.274233e-03 8.072370e-03 1 211663525 211663628 104 - 2.125 2.232 0.358
ENSG00000117650 E006 71.4390757 0.0003886587 1.776648e-01 2.981967e-01 1 211663629 211663652 24 - 1.809 1.880 0.240
ENSG00000117650 E007 17.5643742 0.0205999896 2.908026e-02 7.016262e-02 1 211666430 211667105 676 - 1.298 1.046 -0.898
ENSG00000117650 E008 182.5421832 0.0002631982 7.640865e-01 8.472400e-01 1 211667106 211667231 126 - 2.227 2.247 0.067
ENSG00000117650 E009 232.6090577 0.0011019723 5.043361e-02 1.099419e-01 1 211669113 211669332 220 - 2.351 2.305 -0.152
ENSG00000117650 E010 0.5922303 0.0179723115 9.199407e-01 9.534385e-01 1 211669333 211669519 187 - 0.205 0.184 -0.186
ENSG00000117650 E011 164.9509840 0.0026136060 7.368986e-02 1.494345e-01 1 211670281 211670393 113 - 2.207 2.147 -0.199
ENSG00000117650 E012 88.3874673 0.0003677885 1.789904e-02 4.691978e-02 1 211670394 211670407 14 - 1.946 1.859 -0.293
ENSG00000117650 E013 1.6283978 0.0084853118 4.464696e-01 5.879503e-01 1 211671169 211671201 33 - 0.448 0.313 -0.776
ENSG00000117650 E014 135.4984173 0.0002929833 2.966104e-03 1.016203e-02 1 211671202 211671284 83 - 2.130 2.042 -0.295
ENSG00000117650 E015 248.4397376 0.0001909203 4.202385e-12 9.303836e-11 1 211673483 211673723 241 - 2.410 2.249 -0.539
ENSG00000117650 E016 207.2546953 0.0007212030 1.688514e-09 2.398111e-08 1 211674296 211674513 218 - 2.334 2.167 -0.555
ENSG00000117650 E017 133.7349090 0.0002929054 1.136267e-06 9.324290e-06 1 211675384 211675647 264 - 2.143 1.987 -0.524