ENSG00000117533

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000236192 ENSG00000117533 HEK293_OSMI2_2hA HEK293_TMG_2hB VAMP4 protein_coding protein_coding 6.625743 5.448251 7.696173 0.8121547 0.3798264 0.4975758 1.3355283 0.6218951 2.1516455 0.09411977 0.1483916 1.7743730 0.18554167 0.11426667 0.2793333 0.16506667 3.501504e-06 3.501504e-06 FALSE FALSE
ENST00000367740 ENSG00000117533 HEK293_OSMI2_2hA HEK293_TMG_2hB VAMP4 protein_coding protein_coding 6.625743 5.448251 7.696173 0.8121547 0.3798264 0.4975758 0.2580814 0.2848663 0.0000000 0.15175706 0.0000000 -4.8819892 0.03676667 0.05426667 0.0000000 -0.05426667 8.986934e-02 3.501504e-06 FALSE TRUE
ENST00000474047 ENSG00000117533 HEK293_OSMI2_2hA HEK293_TMG_2hB VAMP4 protein_coding nonsense_mediated_decay 6.625743 5.448251 7.696173 0.8121547 0.3798264 0.4975758 0.9047782 0.0605405 1.8668490 0.06054050 0.3628786 4.7337170 0.11325000 0.01540000 0.2466667 0.23126667 1.246716e-02 3.501504e-06 TRUE TRUE
MSTRG.2587.3 ENSG00000117533 HEK293_OSMI2_2hA HEK293_TMG_2hB VAMP4 protein_coding   6.625743 5.448251 7.696173 0.8121547 0.3798264 0.4975758 2.1439309 1.4397577 2.3794493 0.13139864 0.2658942 0.7208663 0.31298333 0.27086667 0.3078667 0.03700000 6.959739e-01 3.501504e-06 TRUE TRUE
MSTRG.2587.6 ENSG00000117533 HEK293_OSMI2_2hA HEK293_TMG_2hB VAMP4 protein_coding   6.625743 5.448251 7.696173 0.8121547 0.3798264 0.4975758 1.5874469 2.7890127 0.6488042 0.61276515 0.2574389 -2.0869964 0.30258750 0.50076667 0.0823000 -0.41846667 8.752982e-05 3.501504e-06   FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000117533 E001 57.526412 0.0004897040 5.371986e-01 6.688158e-01 1 171700160 171701091 932 - 1.736 1.771 0.118
ENSG00000117533 E002 61.938142 0.0005784161 2.146342e-12 4.986121e-11 1 171701092 171702084 993 - 1.868 1.512 -1.206
ENSG00000117533 E003 18.465747 0.0086942520 3.415715e-04 1.546525e-03 1 171702085 171702967 883 - 1.362 0.999 -1.293
ENSG00000117533 E004 2.459025 0.0293673441 6.685076e-01 7.754801e-01 1 171702968 171702982 15 - 0.549 0.477 -0.347
ENSG00000117533 E005 29.291419 0.0009557252 2.620446e-04 1.224890e-03 1 171702983 171703949 967 - 1.529 1.268 -0.903
ENSG00000117533 E006 29.977045 0.0007309896 6.626222e-02 1.371589e-01 1 171703950 171704386 437 - 1.428 1.551 0.421
ENSG00000117533 E007 26.248234 0.0007876142 7.776048e-01 8.569004e-01 1 171704387 171704504 118 - 1.421 1.407 -0.049
ENSG00000117533 E008 19.977936 0.0009742894 2.080355e-01 3.361254e-01 1 171704505 171704534 30 - 1.268 1.371 0.360
ENSG00000117533 E009 16.771178 0.0022139784 1.836727e-01 3.058122e-01 1 171706367 171706418 52 - 1.193 1.312 0.418
ENSG00000117533 E010 35.098802 0.0007123868 9.362576e-01 9.639005e-01 1 171709665 171709744 80 - 1.534 1.545 0.036
ENSG00000117533 E011 30.809466 0.0010408094 4.939455e-01 6.309711e-01 1 171710714 171710759 46 - 1.495 1.455 -0.135
ENSG00000117533 E012 29.473846 0.0007413147 1.441158e-01 2.536113e-01 1 171710760 171710814 55 - 1.492 1.399 -0.321
ENSG00000117533 E013 26.218711 0.0007905742 7.273638e-01 8.201024e-01 1 171719171 171719218 48 - 1.418 1.399 -0.066
ENSG00000117533 E014 16.200950 0.0027471398 4.192450e-01 5.624899e-01 1 171719219 171719221 3 - 1.232 1.162 -0.249
ENSG00000117533 E015 27.188538 0.0007714072 1.049645e-01 1.980401e-01 1 171728524 171728570 47 - 1.385 1.499 0.393
ENSG00000117533 E016 10.789295 0.0439240935 9.632619e-11 1.704053e-09 1 171732183 171732353 171 - 0.494 1.426 3.601
ENSG00000117533 E017 42.751296 0.0005585515 1.214546e-05 7.912925e-05 1 171738349 171738463 115 - 1.526 1.760 0.797
ENSG00000117533 E018 23.879013 0.0008171587 8.512671e-03 2.506878e-02 1 171741910 171742074 165 - 1.302 1.492 0.658