ENSG00000117528

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000315713 ENSG00000117528 HEK293_OSMI2_2hA HEK293_TMG_2hB ABCD3 protein_coding protein_coding 32.73273 9.997016 52.23673 1.103486 1.80514 2.384329 4.25554 2.422485 7.431653 0.4189340 0.6212787 1.613192 0.1557458 0.2413 0.1417667 -0.09953333 0.01087261 0.01087261 FALSE  
ENST00000370214 ENSG00000117528 HEK293_OSMI2_2hA HEK293_TMG_2hB ABCD3 protein_coding protein_coding 32.73273 9.997016 52.23673 1.103486 1.80514 2.384329 26.50970 6.899667 41.443216 0.4483127 0.9750803 2.584796 0.7779750 0.6971 0.7940000 0.09690000 0.10793210 0.01087261 FALSE  
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000117528 E001 52.2810672 0.0326683153 7.096760e-01 8.069939e-01 1 94418389 94418454 66 + 1.579 1.662 0.282
ENSG00000117528 E002 68.5980230 0.0222945155 5.697358e-01 6.962633e-01 1 94418455 94418478 24 + 1.693 1.787 0.320
ENSG00000117528 E003 124.8006560 0.0074994563 8.071098e-01 8.774825e-01 1 94418479 94418588 110 + 1.960 1.995 0.116
ENSG00000117528 E004 0.5953508 0.0236185779 1.071268e-01 2.012959e-01 1 94418831 94418951 121 + 0.100 0.421 2.661
ENSG00000117528 E005 0.1515154 0.0435981336 1.000000e+00   1 94432587 94432652 66 + 0.053 0.001 -5.829
ENSG00000117528 E006 6.5094119 0.0027167538 8.525464e-01 9.086291e-01 1 94455733 94455804 72 + 0.752 0.828 0.302
ENSG00000117528 E007 104.9268693 0.0048077014 5.469370e-01 6.770212e-01 1 94458607 94458643 37 + 1.889 1.906 0.058
ENSG00000117528 E008 183.0807337 0.0006278264 5.811945e-01 7.057777e-01 1 94464775 94464873 99 + 2.126 2.162 0.118
ENSG00000117528 E009 206.0827487 0.0002945285 5.809779e-01 7.056096e-01 1 94467919 94468007 89 + 2.170 2.244 0.247
ENSG00000117528 E010 0.3729606 0.0331014815 2.109347e-01 3.396394e-01 1 94472173 94472242 70 + 0.053 0.260 2.668
ENSG00000117528 E011 216.8150311 0.0011052845 3.804557e-01 5.247323e-01 1 94473766 94473835 70 + 2.191 2.277 0.288
ENSG00000117528 E012 221.9685955 0.0001924276 7.570451e-01 8.420023e-01 1 94475143 94475240 98 + 2.204 2.270 0.219
ENSG00000117528 E013 197.0821284 0.0002399335 6.126320e-01 7.315201e-01 1 94475614 94475737 124 + 2.151 2.224 0.243
ENSG00000117528 E014 133.1794182 0.0012389915 6.030861e-01 7.239303e-01 1 94478259 94478315 57 + 1.982 2.060 0.260
ENSG00000117528 E015 30.5697873 0.0037532226 5.263055e-15 1.803999e-13 1 94478524 94478723 200 + 1.204 1.816 2.104
ENSG00000117528 E016 6.0647599 0.0031633816 3.375111e-01 4.813924e-01 1 94480222 94480463 242 + 0.752 0.631 -0.505
ENSG00000117528 E017 165.3138731 0.0021893244 7.525890e-04 3.088796e-03 1 94480464 94480606 143 + 2.101 2.001 -0.337
ENSG00000117528 E018 9.3300774 0.0018645539 3.447750e-02 8.067362e-02 1 94481493 94483169 1677 + 0.929 0.631 -1.193
ENSG00000117528 E019 124.6859333 0.0014023455 6.023980e-04 2.542043e-03 1 94483170 94483239 70 + 1.983 1.869 -0.383
ENSG00000117528 E020 126.6913060 0.0002435428 7.370720e-05 3.979370e-04 1 94487542 94487611 70 + 1.991 1.864 -0.426
ENSG00000117528 E021 191.5945059 0.0002250942 7.682546e-10 1.163790e-08 1 94487694 94487791 98 + 2.176 1.994 -0.610
ENSG00000117528 E022 189.9383965 0.0003422850 6.195529e-07 5.381094e-06 1 94487892 94487983 92 + 2.167 2.032 -0.452
ENSG00000117528 E023 208.8936268 0.0024737765 1.802751e-04 8.792302e-04 1 94489725 94489816 92 + 2.204 2.096 -0.360
ENSG00000117528 E024 148.2577296 0.0010599003 2.452033e-02 6.089007e-02 1 94489903 94489975 73 + 2.047 2.004 -0.144
ENSG00000117528 E025 166.0326352 0.0002374453 3.821187e-03 1.263923e-02 1 94491184 94491247 64 + 2.098 2.041 -0.190
ENSG00000117528 E026 195.9422743 0.0009817324 1.214333e-04 6.204701e-04 1 94498602 94498679 78 + 2.174 2.079 -0.319
ENSG00000117528 E027 171.7554141 0.0090705789 1.983436e-01 3.241441e-01 1 94498783 94498848 66 + 2.106 2.080 -0.087
ENSG00000117528 E028 229.7231500 0.0006600028 1.924754e-04 9.316507e-04 1 94498945 94499034 90 + 2.240 2.165 -0.253
ENSG00000117528 E029 256.3907063 0.0003586970 2.912042e-02 7.023626e-02 1 94499495 94499614 120 + 2.279 2.266 -0.041
ENSG00000117528 E030 198.4037071 0.0001751121 4.665751e-01 6.061170e-01 1 94506538 94506642 105 + 2.162 2.193 0.104
ENSG00000117528 E031 2.3355086 0.0145652345 9.027106e-02 1.755503e-01 1 94513474 94515145 1672 + 0.360 0.707 1.663
ENSG00000117528 E032 160.5926467 0.0002151188 4.580355e-02 1.016746e-01 1 94515146 94515202 57 + 2.079 2.057 -0.072
ENSG00000117528 E033 903.2371714 0.0082912207 4.031712e-09 5.343845e-08 1 94517052 94518666 1615 + 2.762 3.048 0.950