ENSG00000117525

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000334047 ENSG00000117525 HEK293_OSMI2_2hA HEK293_TMG_2hB F3 protein_coding protein_coding 0.8997186 0.705559 1.205614 0.06235972 0.2072815 0.7645428 0.55556267 0.18822034 0.94676644 0.09861201 0.09753299 2.2710618 0.63275833 0.2937333 0.80733333 0.5136000 0.1203989715 0.0002014329 FALSE TRUE
ENST00000370207 ENSG00000117525 HEK293_OSMI2_2hA HEK293_TMG_2hB F3 protein_coding protein_coding 0.8997186 0.705559 1.205614 0.06235972 0.2072815 0.7645428 0.16035510 0.42087711 0.02328012 0.10351349 0.02328012 -3.6945441 0.19172083 0.5816667 0.02573333 -0.5559333 0.0002014329 0.0002014329 FALSE TRUE
ENST00000478217 ENSG00000117525 HEK293_OSMI2_2hA HEK293_TMG_2hB F3 protein_coding processed_transcript 0.8997186 0.705559 1.205614 0.06235972 0.2072815 0.7645428 0.08023002 0.00000000 0.18388810 0.00000000 0.18388810 4.2771523 0.05955833 0.0000000 0.11473333 0.1147333 0.8698799655 0.0002014329 FALSE FALSE
MSTRG.1644.3 ENSG00000117525 HEK293_OSMI2_2hA HEK293_TMG_2hB F3 protein_coding   0.8997186 0.705559 1.205614 0.06235972 0.2072815 0.7645428 0.09687164 0.09646159 0.05167958 0.06182418 0.02600347 -0.7874682 0.10577083 0.1246000 0.05220000 -0.0724000 0.7148398547 0.0002014329 TRUE FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000117525 E001 0.0000000       1 94529173 94529309 137 -      
ENSG00000117525 E002 23.9257139 0.001254487 2.382033e-05 0.0001451913 1 94529310 94530396 1087 - 1.273 1.516 0.842
ENSG00000117525 E003 11.5599541 0.007726134 4.038028e-01 0.5475847292 1 94530397 94530596 200 - 1.130 1.048 -0.297
ENSG00000117525 E004 8.1512714 0.002299102 2.562675e-02 0.0631779678 1 94532321 94532480 160 - 1.041 0.785 -0.974
ENSG00000117525 E005 0.2966881 0.029078516 3.862855e-01   1 94532997 94533089 93 - 0.176 0.000 -8.803
ENSG00000117525 E006 3.2527301 0.005117639 1.995376e-02 0.0513890612 1 94533090 94533105 16 - 0.740 0.356 -1.822
ENSG00000117525 E007 9.4439191 0.003448751 2.453069e-02 0.0609112544 1 94533106 94533268 163 - 1.088 0.842 -0.920
ENSG00000117525 E008 8.7333956 0.002401291 2.008980e-01 0.3273092752 1 94535965 94536164 200 - 1.031 0.891 -0.522
ENSG00000117525 E009 4.3878068 0.004059470 7.972997e-01 0.8706688461 1 94540257 94540368 112 - 0.720 0.753 0.134
ENSG00000117525 E010 1.5822971 0.010526241 7.221779e-02 0.1470316721 1 94540572 94541066 495 - 0.243 0.550 1.765
ENSG00000117525 E011 4.1589576 0.004130962 1.211004e-01 0.2215088025 1 94541537 94541759 223 - 0.602 0.814 0.879