ENSG00000117519

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000370206 ENSG00000117519 HEK293_OSMI2_2hA HEK293_TMG_2hB CNN3 protein_coding protein_coding 42.05079 25.87504 66.30744 4.100776 1.487088 1.35727 36.739339 24.79860358 58.233879 3.21374492 1.0354768 1.231266 0.87907500 0.9663333 0.8786667 -0.08766667 2.425680e-01 1.858166e-16 FALSE  
ENST00000487539 ENSG00000117519 HEK293_OSMI2_2hA HEK293_TMG_2hB CNN3 protein_coding processed_transcript 42.05079 25.87504 66.30744 4.100776 1.487088 1.35727 2.828587 0.04055307 5.608715 0.04055307 0.5736274 6.796298 0.05265833 0.0012000 0.0849000 0.08370000 1.858166e-16 1.858166e-16 FALSE  
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000117519 E001 0.0000000       1 94896949 94896956 8 -      
ENSG00000117519 E002 997.2915740 0.0031676870 2.088541e-13 5.672658e-12 1 94896957 94897867 911 - 2.895 3.103 0.691
ENSG00000117519 E003 362.4783504 0.0001760797 2.085748e-01 3.367754e-01 1 94897868 94897946 79 - 2.538 2.549 0.037
ENSG00000117519 E004 369.0166523 0.0001767197 6.022189e-01 7.232155e-01 1 94897947 94898051 105 - 2.541 2.566 0.086
ENSG00000117519 E005 258.4400844 0.0023388114 8.416290e-02 1.660703e-01 1 94898052 94898083 32 - 2.400 2.376 -0.081
ENSG00000117519 E006 460.6180705 0.0001582088 6.404982e-04 2.682209e-03 1 94899371 94899517 147 - 2.651 2.627 -0.082
ENSG00000117519 E007 420.9007679 0.0001266817 2.514447e-03 8.807514e-03 1 94901669 94901785 117 - 2.611 2.591 -0.064
ENSG00000117519 E008 2.5284650 0.0777920963 1.132294e-01 2.102357e-01 1 94901786 94901873 88 - 0.617 0.302 -1.642
ENSG00000117519 E009 304.5555017 0.0002037105 1.708469e-01 2.892726e-01 1 94902121 94902177 57 - 2.462 2.467 0.017
ENSG00000117519 E010 332.0577935 0.0009411187 6.504783e-04 2.717695e-03 1 94902178 94902258 81 - 2.517 2.463 -0.179
ENSG00000117519 E011 280.3328356 0.0030131529 2.524460e-03 8.835889e-03 1 94903122 94903188 67 - 2.451 2.373 -0.259
ENSG00000117519 E012 2.2228062 0.0065265081 2.134631e-02 5.431752e-02 1 94903189 94903321 133 - 0.595 0.177 -2.541
ENSG00000117519 E013 320.2368395 0.0016548296 2.084229e-02 5.325390e-02 1 94903403 94903524 122 - 2.496 2.460 -0.121
ENSG00000117519 E014 0.1817044 0.0445445109 2.138083e-01   1 94924174 94924447 274 - 0.000 0.178 11.392
ENSG00000117519 E015 0.4355181 0.6234654747 5.998061e-01 7.211491e-01 1 94925633 94925808 176 - 0.211 0.000 -10.712
ENSG00000117519 E016 324.5131942 0.0021425684 8.688183e-03 2.550675e-02 1 94926838 94927223 386 - 2.505 2.455 -0.168