ENSG00000117069

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000477717 ENSG00000117069 HEK293_OSMI2_2hA HEK293_TMG_2hB ST6GALNAC5 protein_coding protein_coding 1.209873 1.486067 1.485551 0.04783905 0.2098401 -0.0004978049 0.0673701 0.0000000 0.20576464 0.0000000 0.03183531 4.4313866 0.07461250 0.0000000 0.14956667 0.14956667 0.0004757715 0.0004757715 TRUE TRUE
ENST00000480428 ENSG00000117069 HEK293_OSMI2_2hA HEK293_TMG_2hB ST6GALNAC5 protein_coding processed_transcript 1.209873 1.486067 1.485551 0.04783905 0.2098401 -0.0004978049 0.5201324 0.5886338 0.97223911 0.1546179 0.09863031 0.7144004 0.37535417 0.3902667 0.67706667 0.28680000 0.2378486062 0.0004757715 FALSE FALSE
ENST00000488940 ENSG00000117069 HEK293_OSMI2_2hA HEK293_TMG_2hB ST6GALNAC5 protein_coding processed_transcript 1.209873 1.486067 1.485551 0.04783905 0.2098401 -0.0004978049 0.1020353 0.0000000 0.07008376 0.0000000 0.07008376 3.0015097 0.06910833 0.0000000 0.03706667 0.03706667 0.8179258651 0.0004757715 FALSE FALSE
ENST00000496845 ENSG00000117069 HEK293_OSMI2_2hA HEK293_TMG_2hB ST6GALNAC5 protein_coding processed_transcript 1.209873 1.486067 1.485551 0.04783905 0.2098401 -0.0004978049 0.4831392 0.8518504 0.23746384 0.1075712 0.18832244 -1.8002198 0.43612917 0.5789333 0.13630000 -0.44263333 0.0848330297 0.0004757715 FALSE FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000117069 E001 2.1263661 0.069023277 2.327782e-01 3.657342e-01 1 76867480 76867481 2 + 0.364 0.557 0.990
ENSG00000117069 E002 9.7205963 0.001989885 1.422372e-01 2.510823e-01 1 76867482 76867690 209 + 0.965 1.066 0.372
ENSG00000117069 E003 11.1390844 0.013855168 7.353487e-02 1.491793e-01 1 76868497 76868742 246 + 0.995 1.134 0.506
ENSG00000117069 E004 8.4971442 0.002410319 3.498454e-08 3.886721e-07 1 76868743 76869074 332 + 0.560 1.152 2.328
ENSG00000117069 E005 3.3839035 0.010916570 2.025079e-01 3.293487e-01 1 76871268 76871383 116 + 0.560 0.714 0.668
ENSG00000117069 E006 0.0000000       1 76995457 76995520 64 +      
ENSG00000117069 E007 2.7260891 0.005968468 2.218940e-02 5.606345e-02 1 77044204 77044613 410 + 0.748 0.359 -1.837
ENSG00000117069 E008 0.4355181 0.524066009 2.670527e-01 4.053386e-01 1 77050258 77050261 4 + 0.297 0.000 -10.454
ENSG00000117069 E009 1.9332254 0.371685871 5.028504e-01 6.388550e-01 1 77050262 77050365 104 + 0.595 0.303 -1.538
ENSG00000117069 E010 13.9835365 0.024441170 4.100224e-08 4.500435e-07 1 77062975 77067546 4572 + 1.398 0.813 -2.126