ENSG00000117013

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000443478 ENSG00000117013 HEK293_OSMI2_2hA HEK293_TMG_2hB KCNQ4 protein_coding protein_coding 0.9560583 1.246043 0.6047089 0.1404602 0.02403338 -1.03091 0.1670853 0.3681110 0.0000000 0.19170263 0.00000000 -5.240738 0.1488000 0.2764667 0.0000000 -0.2764667 0.27445914 0.03093491 FALSE TRUE
ENST00000506017 ENSG00000117013 HEK293_OSMI2_2hA HEK293_TMG_2hB KCNQ4 protein_coding processed_transcript 0.9560583 1.246043 0.6047089 0.1404602 0.02403338 -1.03091 0.5090597 0.7454592 0.2579185 0.08725211 0.04933300 -1.495560 0.5150542 0.6075000 0.4258667 -0.1816333 0.42652994 0.03093491 FALSE TRUE
ENST00000509682 ENSG00000117013 HEK293_OSMI2_2hA HEK293_TMG_2hB KCNQ4 protein_coding protein_coding 0.9560583 1.246043 0.6047089 0.1404602 0.02403338 -1.03091 0.2571712 0.1324725 0.3467905 0.07364203 0.04802815 1.324394 0.3007292 0.1160333 0.5741333 0.4581000 0.03093491 0.03093491 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000117013 E001 0.000000       1 40783787 40784093 307 +      
ENSG00000117013 E002 1.186537 0.012321790 0.0242146143 0.0602541050 1 40784094 40784407 314 + 0.543 0.179 -2.287
ENSG00000117013 E003 3.625473 0.023836493 0.0341365610 0.0800287088 1 40817265 40817355 91 + 0.806 0.519 -1.226
ENSG00000117013 E004 3.979330 0.004341457 0.1463155279 0.2566601340 1 40818164 40818290 127 + 0.776 0.609 -0.699
ENSG00000117013 E005 4.810532 0.005794991 0.9817683774 0.9926432207 1 40818505 40818680 176 + 0.712 0.748 0.150
ENSG00000117013 E006 0.000000       1 40818964 40818990 27 +      
ENSG00000117013 E007 4.244820 0.009664750 0.6468705765 0.7586682975 1 40819347 40819472 126 + 0.712 0.684 -0.113
ENSG00000117013 E008 4.250108 0.004244393 0.0212394167 0.0540927563 1 40819875 40819985 111 + 0.859 0.581 -1.147
ENSG00000117013 E009 4.866358 0.026762611 0.0702923018 0.1438453570 1 40820165 40820260 96 + 0.859 0.640 -0.886
ENSG00000117013 E010 3.982753 0.036783162 0.0608341465 0.1280424690 1 40822314 40822402 89 + 0.806 0.556 -1.055
ENSG00000117013 E011 4.006395 0.004557949 0.9425329147 0.9679362141 1 40824097 40824258 162 + 0.635 0.684 0.209
ENSG00000117013 E012 0.732103 0.020625646 0.6590660282 0.7679793445 1 40826638 40826694 57 + 0.151 0.247 0.888
ENSG00000117013 E013 7.563133 0.003316980 0.6620103341 0.7703772268 1 40831084 40831304 221 + 0.906 0.898 -0.031
ENSG00000117013 E014 6.780693 0.019338568 0.5810279471 0.7056472051 1 40833014 40833113 100 + 0.883 0.854 -0.110
ENSG00000117013 E015 6.184086 0.003339429 0.2374357137 0.3711956070 1 40834967 40835098 132 + 0.906 0.803 -0.396
ENSG00000117013 E016 6.883909 0.002510605 0.4234903727 0.5665341782 1 40837665 40837794 130 + 0.906 0.853 -0.202
ENSG00000117013 E017 66.273271 0.016965949 0.0000493307 0.0002783569 1 40838311 40840452 2142 + 1.619 1.871 0.851