ENSG00000116771

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000375826 ENSG00000116771 HEK293_OSMI2_2hA HEK293_TMG_2hB AGMAT protein_coding protein_coding 2.160855 3.806873 1.641338 0.07007121 0.01733854 -1.208756 0.9455328 1.27917073 0.8060679 0.09774634 0.05526500 -0.6596823 0.47766667 0.33533333 0.49110000 0.1557667 0.10130985 0.03865926 FALSE TRUE
MSTRG.361.1 ENSG00000116771 HEK293_OSMI2_2hA HEK293_TMG_2hB AGMAT protein_coding   2.160855 3.806873 1.641338 0.07007121 0.01733854 -1.208756 1.1358061 2.46216784 0.7222624 0.01500520 0.09186428 -1.7553441 0.47613333 0.64730000 0.43963333 -0.2076667 0.03865926 0.03865926 TRUE TRUE
MSTRG.361.2 ENSG00000116771 HEK293_OSMI2_2hA HEK293_TMG_2hB AGMAT protein_coding   2.160855 3.806873 1.641338 0.07007121 0.01733854 -1.208756 0.0795164 0.06553478 0.1130079 0.01970816 0.04234329 0.7035380 0.04619167 0.01736667 0.06926667 0.0519000 0.12845462 0.03865926 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000116771 E001 0.1817044 0.0406904333 1.0000000000   1 15529041 15529089 49 - 0.001 0.084 6.893
ENSG00000116771 E002 3.9800270 0.0481306600 0.0003585426 0.001613071 1 15570362 15571698 1337 - 0.996 0.399 -2.565
ENSG00000116771 E003 23.6109230 0.0011516528 0.1218889262 0.222606457 1 15571699 15572914 1216 - 1.426 1.312 -0.396
ENSG00000116771 E004 34.5358353 0.0006323692 0.0227629877 0.057254112 1 15572915 15573587 673 - 1.597 1.461 -0.466
ENSG00000116771 E005 29.3725500 0.0069766611 0.1874467195 0.310524040 1 15573588 15573724 137 - 1.507 1.410 -0.333
ENSG00000116771 E006 29.5783069 0.0007477216 0.5322714890 0.664635948 1 15574757 15574841 85 - 1.410 1.447 0.130
ENSG00000116771 E007 37.3359179 0.0007129699 0.0097935376 0.028254444 1 15577685 15577864 180 - 1.410 1.566 0.537
ENSG00000116771 E008 36.7388051 0.0007144416 0.0162911562 0.043367707 1 15578859 15579054 196 - 1.410 1.556 0.503
ENSG00000116771 E009 23.3233879 0.0009325698 0.8784407898 0.925918650 1 15580094 15580142 49 - 1.327 1.334 0.025
ENSG00000116771 E010 34.9505228 0.0006626427 0.2722089628 0.411063676 1 15583193 15583395 203 - 1.457 1.521 0.219
ENSG00000116771 E011 17.2414778 0.0017367389 0.8422814109 0.901705939 1 15584696 15585051 356 - 1.225 1.204 -0.073