ENSG00000116747

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000367441 ENSG00000116747 HEK293_OSMI2_2hA HEK293_TMG_2hB RO60 protein_coding protein_coding 8.106757 1.48412 13.13289 0.03785267 0.7319065 3.136915 2.381850005 0.00000000 3.3992350 0.00000000 0.2797483 8.4133042 0.169070833 0.00000000 0.25843333 0.25843333 2.929887e-12 2.929887e-12 FALSE TRUE
ENST00000367444 ENSG00000116747 HEK293_OSMI2_2hA HEK293_TMG_2hB RO60 protein_coding protein_coding 8.106757 1.48412 13.13289 0.03785267 0.7319065 3.136915 0.009672614 0.07738091 0.0000000 0.07738091 0.0000000 -3.1273182 0.006608333 0.05286667 0.00000000 -0.05286667 3.512370e-01 2.929887e-12 FALSE TRUE
ENST00000400968 ENSG00000116747 HEK293_OSMI2_2hA HEK293_TMG_2hB RO60 protein_coding protein_coding 8.106757 1.48412 13.13289 0.03785267 0.7319065 3.136915 2.012051973 0.30346001 3.8081741 0.15192647 0.1729187 3.6065296 0.244033333 0.20960000 0.29043333 0.08083333 7.476149e-01 2.929887e-12 FALSE TRUE
ENST00000432079 ENSG00000116747 HEK293_OSMI2_2hA HEK293_TMG_2hB RO60 protein_coding protein_coding 8.106757 1.48412 13.13289 0.03785267 0.7319065 3.136915 1.351812403 0.31875046 2.1670484 0.07086014 0.2356243 2.7273087 0.182187500 0.21293333 0.16400000 -0.04893333 6.848756e-01 2.929887e-12 FALSE TRUE
ENST00000460715 ENSG00000116747 HEK293_OSMI2_2hA HEK293_TMG_2hB RO60 protein_coding processed_transcript 8.106757 1.48412 13.13289 0.03785267 0.7319065 3.136915 0.537339811 0.38487821 0.2852226 0.16204829 0.1671684 -0.4196044 0.162458333 0.25593333 0.02246667 -0.23346667 8.890811e-02 2.929887e-12 FALSE TRUE
ENST00000469214 ENSG00000116747 HEK293_OSMI2_2hA HEK293_TMG_2hB RO60 protein_coding retained_intron 8.106757 1.48412 13.13289 0.03785267 0.7319065 3.136915 0.470876490 0.10907460 1.0934828 0.05911633 0.3380301 3.2121266 0.079350000 0.07556667 0.08630000 0.01073333 9.667234e-01 2.929887e-12   FALSE
ENST00000512587 ENSG00000116747 HEK293_OSMI2_2hA HEK293_TMG_2hB RO60 protein_coding protein_coding 8.106757 1.48412 13.13289 0.03785267 0.7319065 3.136915 0.230369854 0.00000000 0.7805390 0.00000000 0.2566235 6.3047647 0.026033333 0.00000000 0.05813333 0.05813333 3.323921e-02 2.929887e-12 FALSE FALSE
MSTRG.2788.18 ENSG00000116747 HEK293_OSMI2_2hA HEK293_TMG_2hB RO60 protein_coding   8.106757 1.48412 13.13289 0.03785267 0.7319065 3.136915 0.138357143 0.16611126 0.2307021 0.09023729 0.2307021 0.4507616 0.032395833 0.10923333 0.01753333 -0.09170000 3.540736e-01 2.929887e-12 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000116747 E001 4.7889342 0.0322469205 6.757531e-01 7.809305e-01 1 193059454 193059594 141 + 0.644 0.641 -0.013
ENSG00000116747 E002 3.8739941 0.0378664370 6.456438e-02 1.343165e-01 1 193059595 193059611 17 + 0.618 0.265 -1.909
ENSG00000116747 E003 3.8803368 0.0373560226 6.438158e-02 1.340097e-01 1 193059612 193059618 7 + 0.619 0.265 -1.910
ENSG00000116747 E004 4.2532974 0.0278174485 1.869804e-01 3.099322e-01 1 193059619 193059622 4 + 0.632 0.428 -0.964
ENSG00000116747 E005 4.6908918 0.0193208295 1.239265e-01 2.255680e-01 1 193059623 193059627 5 + 0.668 0.428 -1.122
ENSG00000116747 E006 12.0442831 0.0114400616 4.475066e-01 5.888806e-01 1 193059628 193059658 31 + 0.987 0.973 -0.052
ENSG00000116747 E007 20.7456355 0.0009245302 1.616730e-01 2.772886e-01 1 193059659 193059704 46 + 1.211 1.159 -0.186
ENSG00000116747 E008 19.1934085 0.0009554193 4.530406e-01 5.938271e-01 1 193059705 193059705 1 + 1.169 1.184 0.052
ENSG00000116747 E009 30.3947095 0.0006916729 4.436473e-02 9.904972e-02 1 193059706 193059776 71 + 1.373 1.289 -0.291
ENSG00000116747 E010 9.0384923 0.0024859658 2.000824e-01 3.263593e-01 1 193059874 193060033 160 + 0.825 1.076 0.942
ENSG00000116747 E011 3.2881290 0.0049968899 3.523089e-01 4.965803e-01 1 193060034 193060056 23 + 0.480 0.716 1.055
ENSG00000116747 E012 8.7064315 0.0057412023 4.635183e-01 6.033168e-01 1 193060057 193060222 166 + 0.825 1.010 0.701
ENSG00000116747 E013 0.9232601 0.0964839370 3.240172e-01 4.673677e-01 1 193060223 193060283 61 + 0.178 0.427 1.727
ENSG00000116747 E014 0.2987644 0.0274877070 1.000000e+00   1 193069016 193069033 18 + 0.098 0.000 -21.632
ENSG00000116747 E015 32.7013786 0.0006442429 8.358460e-03 2.468718e-02 1 193069034 193069181 148 + 1.408 1.270 -0.482
ENSG00000116747 E016 22.7309891 0.0028018871 1.908760e-03 6.942933e-03 1 193069182 193069231 50 + 1.271 1.010 -0.936
ENSG00000116747 E017 39.7001343 0.0074498433 3.067004e-04 1.406659e-03 1 193069232 193069444 213 + 1.502 1.250 -0.873
ENSG00000116747 E018 35.8736079 0.0005937786 1.777203e-02 4.664852e-02 1 193069445 193069634 190 + 1.443 1.342 -0.351
ENSG00000116747 E019 2.5228303 0.0187076254 3.285716e-02 7.752276e-02 1 193070554 193070619 66 + 0.498 0.000 -24.654
ENSG00000116747 E020 56.5491579 0.0061457969 5.547651e-03 1.741196e-02 1 193075820 193076040 221 + 1.638 1.517 -0.414
ENSG00000116747 E021 52.5583872 0.0005558782 2.168884e-02 5.502799e-02 1 193076501 193076647 147 + 1.598 1.539 -0.202
ENSG00000116747 E022 50.3247458 0.0004521730 2.027577e-02 5.206548e-02 1 193076913 193077050 138 + 1.581 1.517 -0.219
ENSG00000116747 E023 33.8561813 0.0006370166 3.993771e-02 9.091988e-02 1 193081364 193081480 117 + 1.419 1.342 -0.267
ENSG00000116747 E024 43.5371844 0.0005366508 3.827793e-03 1.265866e-02 1 193082186 193082299 114 + 1.529 1.404 -0.432
ENSG00000116747 E025 54.8383374 0.0004614551 2.938820e-02 7.074971e-02 1 193082562 193082708 147 + 1.616 1.569 -0.158
ENSG00000116747 E026 37.6202958 0.0006008703 3.369016e-01 4.807584e-01 1 193084579 193084657 79 + 1.447 1.470 0.079
ENSG00000116747 E027 29.6437962 0.0016731780 2.125801e-02 5.413137e-02 1 193084658 193084698 41 + 1.368 1.250 -0.414
ENSG00000116747 E028 40.8273628 0.0005201052 2.423666e-02 6.030025e-02 1 193084699 193084831 133 + 1.495 1.418 -0.267
ENSG00000116747 E029 50.2588469 0.0004437972 3.898825e-01 5.339324e-01 1 193084832 193084988 157 + 1.566 1.607 0.138
ENSG00000116747 E030 36.1197670 0.0005881690 3.044293e-01 4.464354e-01 1 193084989 193085040 52 + 1.429 1.445 0.054
ENSG00000116747 E031 649.8524996 0.0102304435 2.409430e-03 8.490212e-03 1 193085041 193091219 6179 + 2.637 2.825 0.625
ENSG00000116747 E032 36.4209413 0.0338869033 7.754299e-17 3.380963e-15 1 193091651 193091967 317 + 1.070 2.060 3.406