ENSG00000116539

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000368346 ENSG00000116539 HEK293_OSMI2_2hA HEK293_TMG_2hB ASH1L protein_coding protein_coding 9.65624 7.894588 10.31382 0.9360571 1.755997 0.3852141 0.8338371 0.02049906 1.6409353 0.02049906 0.28192186 5.75837493 0.08716667 0.00340000 0.17470000 0.171300000 4.805640e-05 2.483652e-16 FALSE TRUE
ENST00000384987 ENSG00000116539 HEK293_OSMI2_2hA HEK293_TMG_2hB ASH1L protein_coding miRNA 9.65624 7.894588 10.31382 0.9360571 1.755997 0.3852141 0.3320807 0.00000000 1.7012794 0.00000000 1.70127938 7.41893150 0.02508333 0.00000000 0.12306667 0.123066667 8.710309e-01 2.483652e-16   FALSE
ENST00000478837 ENSG00000116539 HEK293_OSMI2_2hA HEK293_TMG_2hB ASH1L protein_coding retained_intron 9.65624 7.894588 10.31382 0.9360571 1.755997 0.3852141 0.5097958 0.19707952 0.1933214 0.09881849 0.09702047 -0.02642263 0.05312500 0.02720000 0.02256667 -0.004633333 9.639833e-01 2.483652e-16 FALSE TRUE
ENST00000492987 ENSG00000116539 HEK293_OSMI2_2hA HEK293_TMG_2hB ASH1L protein_coding nonsense_mediated_decay 9.65624 7.894588 10.31382 0.9360571 1.755997 0.3852141 0.5388969 0.54910992 0.1706938 0.28060077 0.08682631 -1.62958501 0.05453750 0.06223333 0.01653333 -0.045700000 7.442293e-01 2.483652e-16 TRUE TRUE
ENST00000677825 ENSG00000116539 HEK293_OSMI2_2hA HEK293_TMG_2hB ASH1L protein_coding protein_coding 9.65624 7.894588 10.31382 0.9360571 1.755997 0.3852141 0.5637970 0.17038335 0.7479990 0.17038335 0.74799903 2.07112984 0.05340833 0.01916667 0.05410000 0.034933333 1.000000e+00 2.483652e-16 FALSE TRUE
ENST00000677992 ENSG00000116539 HEK293_OSMI2_2hA HEK293_TMG_2hB ASH1L protein_coding protein_coding 9.65624 7.894588 10.31382 0.9360571 1.755997 0.3852141 2.8343773 4.83589959 0.7576578 0.40008798 0.07471210 -2.65822924 0.30299583 0.62523333 0.07510000 -0.550133333 1.332913e-14 2.483652e-16 FALSE TRUE
ENST00000678699 ENSG00000116539 HEK293_OSMI2_2hA HEK293_TMG_2hB ASH1L protein_coding nonsense_mediated_decay 9.65624 7.894588 10.31382 0.9360571 1.755997 0.3852141 1.3136306 1.39387806 1.0566195 0.33090511 0.58898082 -0.39637202 0.13649167 0.17303333 0.12340000 -0.049633333 7.776004e-01 2.483652e-16 TRUE TRUE
MSTRG.2319.1 ENSG00000116539 HEK293_OSMI2_2hA HEK293_TMG_2hB ASH1L protein_coding   9.65624 7.894588 10.31382 0.9360571 1.755997 0.3852141 0.5399722 0.38352199 0.8852380 0.11002781 0.23635361 1.18582702 0.05664167 0.04950000 0.08906667 0.039566667 5.450866e-01 2.483652e-16 FALSE TRUE
MSTRG.2319.14 ENSG00000116539 HEK293_OSMI2_2hA HEK293_TMG_2hB ASH1L protein_coding   9.65624 7.894588 10.31382 0.9360571 1.755997 0.3852141 1.3099652 0.00000000 2.5353846 0.00000000 0.13655200 7.99173983 0.13351667 0.00000000 0.25816667 0.258166667 2.483652e-16 2.483652e-16 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000116539 E001 0.1817044 0.0383284451 2.887428e-01   1 155335223 155335267 45 - 0.000 0.151 13.364
ENSG00000116539 E002 0.7321030 0.0175940462 1.252074e-01 2.274356e-01 1 155335268 155335286 19 - 0.098 0.353 2.302
ENSG00000116539 E003 2.2735384 0.0067846942 3.052585e-05 1.813033e-04 1 155335287 155335300 14 - 0.098 0.778 4.299
ENSG00000116539 E004 370.4334586 0.0088897620 3.743784e-25 4.152509e-23 1 155335301 155337172 1872 - 2.321 2.763 1.469
ENSG00000116539 E005 185.3888534 0.0002329138 3.968728e-16 1.580542e-14 1 155337173 155337751 579 - 2.168 2.360 0.641
ENSG00000116539 E006 142.6530490 0.0033418972 1.085598e-04 5.614767e-04 1 155338089 155338390 302 - 2.089 2.221 0.442
ENSG00000116539 E007 1.0632359 0.0117431834 1.894367e-01 3.130323e-01 1 155338391 155338405 15 - 0.402 0.151 -1.870
ENSG00000116539 E008 59.3263603 0.0004401110 1.157557e-01 2.138906e-01 1 155339328 155339368 41 - 1.747 1.800 0.179
ENSG00000116539 E009 98.8937281 0.0032139184 7.734031e-02 1.552199e-01 1 155341936 155342102 167 - 1.965 2.023 0.194
ENSG00000116539 E010 2.0135764 0.0537932133 9.158906e-01 9.508120e-01 1 155343041 155343313 273 - 0.481 0.488 0.032
ENSG00000116539 E011 76.6588291 0.0003294872 4.713655e-02 1.040681e-01 1 155343314 155343450 137 - 1.852 1.913 0.207
ENSG00000116539 E012 37.8469028 0.0006203401 6.249121e-01 7.411415e-01 1 155343451 155343486 36 - 1.572 1.581 0.031
ENSG00000116539 E013 0.8407440 0.0155790395 8.840075e-01 9.298349e-01 1 155343592 155343603 12 - 0.246 0.264 0.131
ENSG00000116539 E014 41.2743651 0.0011862012 5.637485e-01 6.911802e-01 1 155343604 155343655 52 - 1.609 1.622 0.045
ENSG00000116539 E015 50.7491605 0.0032743436 2.549624e-01 3.917396e-01 1 155343656 155343742 87 - 1.686 1.730 0.148
ENSG00000116539 E016 45.3800970 0.0038103970 1.584701e-02 4.239393e-02 1 155344183 155344273 91 - 1.601 1.723 0.415
ENSG00000116539 E017 1.7379797 0.0091954783 5.608147e-01 6.887390e-01 1 155344274 155344591 318 - 0.402 0.489 0.450
ENSG00000116539 E018 0.6600969 0.0297920237 6.030798e-01 7.239274e-01 1 155345963 155346207 245 - 0.178 0.263 0.715
ENSG00000116539 E019 0.5244761 0.2449014737 9.784201e-01 9.906458e-01 1 155346350 155346382 33 - 0.179 0.153 -0.267
ENSG00000116539 E020 46.6511896 0.0005706318 2.979809e-02 7.156567e-02 1 155346383 155346469 87 - 1.625 1.719 0.318
ENSG00000116539 E021 100.2218831 0.0003434223 5.375111e-03 1.694830e-02 1 155347656 155347901 246 - 1.961 2.041 0.267
ENSG00000116539 E022 30.9306100 0.0006831950 4.317952e-01 5.743092e-01 1 155347902 155347904 3 - 1.484 1.514 0.105
ENSG00000116539 E023 61.7835275 0.0004243745 5.960053e-01 7.180011e-01 1 155349327 155349459 133 - 1.787 1.792 0.016
ENSG00000116539 E024 31.5906364 0.0054515532 9.223141e-01 9.549787e-01 1 155349542 155349596 55 - 1.512 1.503 -0.029
ENSG00000116539 E025 44.6013476 0.0027186697 9.987003e-01 1.000000e+00 1 155352706 155352858 153 - 1.660 1.643 -0.057
ENSG00000116539 E026 57.9798719 0.0009458240 7.317517e-01 8.233445e-01 1 155354473 155354630 158 - 1.778 1.742 -0.120
ENSG00000116539 E027 56.1199365 0.0092642609 6.372573e-01 7.511883e-01 1 155357316 155357410 95 - 1.765 1.723 -0.142
ENSG00000116539 E028 69.0130153 0.0007975339 1.350896e-01 2.412208e-01 1 155357585 155357749 165 - 1.872 1.786 -0.290
ENSG00000116539 E029 50.5023743 0.0004216953 1.965703e-02 5.075465e-02 1 155360301 155360409 109 - 1.757 1.617 -0.473
ENSG00000116539 E030 0.1472490 0.0429978024 8.080053e-01   1 155370147 155370503 357 - 0.098 0.000 -12.234
ENSG00000116539 E031 55.6494659 0.0051738156 1.624964e-02 4.327427e-02 1 155370504 155370650 147 - 1.806 1.645 -0.545
ENSG00000116539 E032 71.0348662 0.0010202747 4.223102e-04 1.859726e-03 1 155370777 155370983 207 - 1.917 1.736 -0.611
ENSG00000116539 E033 49.6940083 0.0055709385 3.447088e-02 8.066212e-02 1 155378281 155378389 109 - 1.753 1.602 -0.514
ENSG00000116539 E034 31.1759440 0.0069729250 5.652427e-01 6.923673e-01 1 155378503 155378548 46 - 1.519 1.458 -0.207
ENSG00000116539 E035 34.4446356 0.0071309512 1.549405e-01 2.683323e-01 1 155380043 155380116 74 - 1.584 1.465 -0.409
ENSG00000116539 E036 0.1472490 0.0429978024 8.080053e-01   1 155380117 155380131 15 - 0.098 0.000 -12.234
ENSG00000116539 E037 33.3194296 0.0006332212 7.307322e-03 2.201785e-02 1 155395459 155395553 95 - 1.598 1.408 -0.651
ENSG00000116539 E038 47.6696170 0.0005065018 1.947197e-03 7.062946e-03 1 155415744 155415923 180 - 1.747 1.562 -0.631
ENSG00000116539 E039 107.1906399 0.0052296930 1.168688e-05 7.646498e-05 1 155438327 155439068 742 - 2.110 1.879 -0.774
ENSG00000116539 E040 0.1472490 0.0429978024 8.080053e-01   1 155440490 155440502 13 - 0.098 0.000 -12.234
ENSG00000116539 E041 0.7352129 0.1248633184 4.727876e-01 6.118339e-01 1 155440503 155440593 91 - 0.304 0.150 -1.295
ENSG00000116539 E042 34.3413689 0.0006031496 2.257150e-05 1.382376e-04 1 155459797 155459898 102 - 1.640 1.348 -1.005
ENSG00000116539 E043 0.7406253 0.0155178248 6.500394e-02 1.350453e-01 1 155462453 155462599 147 - 0.356 0.000 -14.556
ENSG00000116539 E044 445.8811713 0.0317372485 8.223450e-08 8.491813e-07 1 155477886 155482449 4564 - 2.773 2.373 -1.331
ENSG00000116539 E045 0.0000000       1 155520225 155520356 132 -      
ENSG00000116539 E046 55.5822309 0.0388877997 5.845260e-07 5.104665e-06 1 155521100 155521618 519 - 1.904 1.355 -1.872
ENSG00000116539 E047 14.2072907 0.0016515099 1.849461e-09 2.607925e-08 1 155562153 155562291 139 - 1.359 0.677 -2.543
ENSG00000116539 E048 6.7473321 0.0036596150 1.245608e-04 6.346873e-04 1 155562292 155562295 4 - 1.048 0.489 -2.286
ENSG00000116539 E049 9.5372489 0.0202449467 1.329145e-03 5.072676e-03 1 155562296 155562778 483 - 1.160 0.713 -1.693
ENSG00000116539 E050 3.0238094 0.0050442058 3.245196e-02 7.672142e-02 1 155562779 155563072 294 - 0.726 0.352 -1.789
ENSG00000116539 E051 0.6234514 0.4460081946 8.112267e-01 8.802056e-01 1 155563073 155564202 1130 - 0.246 0.152 -0.862