ENSG00000116521

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000302631 ENSG00000116521 HEK293_OSMI2_2hA HEK293_TMG_2hB SCAMP3 protein_coding protein_coding 173.6461 292.2631 95.97595 5.556658 2.81501 -1.606422 99.246948 158.22685 57.8684542 9.774602 1.0256530 -1.450987 0.5809375 0.54066667 0.603566667 0.06290000 0.3136537 3.361649e-10 FALSE  
ENST00000355379 ENSG00000116521 HEK293_OSMI2_2hA HEK293_TMG_2hB SCAMP3 protein_coding protein_coding 173.6461 292.2631 95.97595 5.556658 2.81501 -1.606422 43.900039 78.76342 21.2818938 1.333346 2.2843218 -1.887405 0.2466667 0.26956667 0.221200000 -0.04836667 0.2915977 3.361649e-10 FALSE  
ENST00000478737 ENSG00000116521 HEK293_OSMI2_2hA HEK293_TMG_2hB SCAMP3 protein_coding processed_transcript 173.6461 292.2631 95.97595 5.556658 2.81501 -1.606422 7.418514 19.29121 0.3684772 5.756322 0.3684772 -5.672341 0.0307375 0.06656667 0.004033333 -0.06253333 0.0294376 3.361649e-10 FALSE  
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000116521 E001 8.663319 3.166678e-03 2.576426e-02 6.345649e-02 1 155255979 155255980 2 - 0.606 0.947 1.372
ENSG00000116521 E002 105.155793 4.837489e-03 4.024031e-05 2.317944e-04 1 155255981 155256006 26 - 1.715 1.961 0.830
ENSG00000116521 E003 112.956529 5.144030e-03 1.022312e-03 4.035967e-03 1 155256007 155256014 8 - 1.794 1.985 0.644
ENSG00000116521 E004 313.192444 3.143730e-03 2.931886e-01 4.342303e-01 1 155256015 155256028 14 - 2.360 2.403 0.143
ENSG00000116521 E005 1530.899856 1.869112e-03 1.159638e-03 4.504152e-03 1 155256029 155256240 212 - 3.016 3.096 0.266
ENSG00000116521 E006 2374.142771 9.237028e-04 1.088730e-09 1.603488e-08 1 155256241 155256419 179 - 3.186 3.290 0.346
ENSG00000116521 E007 38.470377 5.457809e-04 3.635889e-12 8.130576e-11 1 155256642 155256673 32 - 1.786 1.375 -1.402
ENSG00000116521 E008 1731.368035 3.131249e-04 5.744272e-10 8.912654e-09 1 155256674 155256791 118 - 3.063 3.150 0.289
ENSG00000116521 E009 786.836851 3.425651e-04 2.507489e-02 6.202894e-02 1 155257285 155257285 1 - 2.756 2.801 0.148
ENSG00000116521 E010 819.796771 2.747224e-04 1.435174e-02 3.902903e-02 1 155257286 155257297 12 - 2.773 2.819 0.154
ENSG00000116521 E011 1576.008263 1.510838e-04 2.894639e-01 4.301430e-01 1 155257298 155257386 89 - 3.079 3.096 0.056
ENSG00000116521 E012 1989.769790 2.626624e-04 8.515927e-03 2.507785e-02 1 155257498 155257657 160 - 3.215 3.188 -0.087
ENSG00000116521 E013 22.097345 2.072752e-02 6.269734e-07 5.439560e-06 1 155258791 155258825 35 - 1.614 1.115 -1.737
ENSG00000116521 E014 1202.584652 1.379712e-04 1.244133e-04 6.340689e-04 1 155258826 155258892 67 - 3.012 2.965 -0.154
ENSG00000116521 E015 1123.269192 8.475922e-05 4.788165e-01 6.172409e-01 1 155258893 155258954 62 - 2.950 2.944 -0.019
ENSG00000116521 E016 1567.630701 2.070652e-04 3.703688e-04 1.659695e-03 1 155260330 155260450 121 - 3.122 3.082 -0.132
ENSG00000116521 E017 18.642115 9.681344e-04 1.423613e-04 7.137354e-04 1 155260451 155260536 86 - 1.433 1.116 -1.114
ENSG00000116521 E018 1486.986229 4.259110e-04 9.870171e-06 6.569722e-05 1 155260537 155260659 123 - 3.114 3.055 -0.193
ENSG00000116521 E019 6.467804 3.992604e-03 3.292814e-10 5.321734e-09 1 155261227 155261278 52 - 1.255 0.491 -3.017
ENSG00000116521 E020 12.377212 1.443640e-03 3.073635e-12 6.965202e-11 1 155261279 155261348 70 - 1.461 0.807 -2.366
ENSG00000116521 E021 22.563611 8.430710e-04 1.180205e-21 9.041093e-20 1 155261349 155261656 308 - 1.721 1.033 -2.398
ENSG00000116521 E022 853.218611 2.081413e-03 2.002347e-04 9.647475e-04 1 155261657 155261734 78 - 2.898 2.808 -0.300
ENSG00000116521 E023 18.508433 1.332483e-03 2.261370e-05 1.384602e-04 1 155261735 155261868 134 - 1.452 1.098 -1.243
ENSG00000116521 E024 11.745504 3.065161e-02 1.185870e-08 1.442067e-07 1 155261946 155262085 140 - 1.452 0.750 -2.562
ENSG00000116521 E025 1406.220222 3.230185e-03 1.345940e-02 3.698747e-02 1 155262086 155262430 345 - 3.096 3.031 -0.216