ENSG00000116459

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000369722 ENSG00000116459 HEK293_OSMI2_2hA HEK293_TMG_2hB ATP5PB protein_coding protein_coding 170.018 98.49687 257.0616 12.12368 1.101622 1.383874 85.01376 63.62370 113.48347 9.724675 3.5248685 0.8347465 0.5348083 0.6419333 0.4416000 -0.20033333 1.404759e-06 5.705709e-07 FALSE TRUE
ENST00000464154 ENSG00000116459 HEK293_OSMI2_2hA HEK293_TMG_2hB ATP5PB protein_coding processed_transcript 170.018 98.49687 257.0616 12.12368 1.101622 1.383874 38.50545 15.86960 59.63603 1.293346 0.6621609 1.9092520 0.2109333 0.1658667 0.2320000 0.06613333 1.800203e-01 5.705709e-07   FALSE
ENST00000493119 ENSG00000116459 HEK293_OSMI2_2hA HEK293_TMG_2hB ATP5PB protein_coding processed_transcript 170.018 98.49687 257.0616 12.12368 1.101622 1.383874 43.66116 17.52811 78.82133 2.446844 4.9741686 2.1682758 0.2385833 0.1772667 0.3064667 0.12920000 5.705709e-07 5.705709e-07   FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000116459 E001 0.6266857 0.0181289730 5.496617e-01 6.793653e-01 1 111448864 111448949 86 + 0.165 0.282 0.980
ENSG00000116459 E002 5.3864210 0.0074010287 3.014969e-01 4.433089e-01 1 111449195 111449391 197 + 0.732 0.895 0.639
ENSG00000116459 E003 56.6746455 0.0004219334 9.357781e-03 2.717690e-02 1 111449392 111449463 72 + 1.788 1.690 -0.332
ENSG00000116459 E004 61.1563857 0.0013294742 8.413431e-04 3.406495e-03 1 111449464 111449464 1 + 1.833 1.694 -0.472
ENSG00000116459 E005 195.8425287 0.0033829213 5.434391e-04 2.322578e-03 1 111449465 111449480 16 + 2.324 2.212 -0.374
ENSG00000116459 E006 229.5995234 0.0024115686 8.406468e-03 2.480423e-02 1 111449481 111449481 1 + 2.377 2.311 -0.220
ENSG00000116459 E007 254.7434643 0.0012688118 3.918494e-04 1.743370e-03 1 111449482 111449483 2 + 2.426 2.349 -0.255
ENSG00000116459 E008 262.8987644 0.0011938017 9.679896e-05 5.071004e-04 1 111449484 111449484 1 + 2.442 2.358 -0.280
ENSG00000116459 E009 307.8362226 0.0011074966 1.251014e-07 1.247307e-06 1 111449485 111449488 4 + 2.520 2.406 -0.383
ENSG00000116459 E010 1500.9993468 0.0002938619 5.045310e-09 6.580961e-08 1 111449489 111449581 93 + 3.183 3.141 -0.142
ENSG00000116459 E011 5.5754766 0.0028919796 8.127953e-01 8.813152e-01 1 111449682 111449836 155 + 0.817 0.812 -0.019
ENSG00000116459 E012 1411.1559457 0.0011606380 2.637154e-04 1.231818e-03 1 111449837 111449873 37 + 3.155 3.112 -0.142
ENSG00000116459 E013 2143.6783628 0.0002039768 4.968338e-04 2.147962e-03 1 111454211 111454356 146 + 3.325 3.318 -0.021
ENSG00000116459 E014 1897.7205436 0.0002200321 1.912681e-02 4.961547e-02 1 111456086 111456226 141 + 3.268 3.271 0.008
ENSG00000116459 E015 984.6427772 0.0003232641 9.535275e-02 1.834218e-01 1 111456227 111456249 23 + 2.983 2.987 0.013
ENSG00000116459 E016 1938.0076801 0.0009491038 5.819485e-01 7.064081e-01 1 111456630 111456755 126 + 3.265 3.294 0.095
ENSG00000116459 E017 2241.4875916 0.0010484427 4.324653e-04 1.899280e-03 1 111459457 111459636 180 + 3.309 3.386 0.256
ENSG00000116459 E018 2467.9049045 0.0014218287 1.784481e-15 6.527603e-14 1 111460917 111462773 1857 + 3.313 3.474 0.535