ENSG00000116266

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000370008 ENSG00000116266 HEK293_OSMI2_2hA HEK293_TMG_2hB STXBP3 protein_coding protein_coding 20.68658 6.187323 31.96197 0.7083364 1.074817 2.367091 17.084017 5.6682896 26.865380 0.69472888 1.3322274 2.242757 0.83081667 0.91456667 0.83960000 -0.07496667 0.02731819 0.02731819 FALSE  
ENST00000485167 ENSG00000116266 HEK293_OSMI2_2hA HEK293_TMG_2hB STXBP3 protein_coding processed_transcript 20.68658 6.187323 31.96197 0.7083364 1.074817 2.367091 1.133135 0.2267944 1.681314 0.03528516 0.1231164 2.836437 0.05462500 0.03633333 0.05260000 0.01626667 0.40840916 0.02731819 FALSE  
ENST00000495245 ENSG00000116266 HEK293_OSMI2_2hA HEK293_TMG_2hB STXBP3 protein_coding retained_intron 20.68658 6.187323 31.96197 0.7083364 1.074817 2.367091 1.445096 0.2125568 2.357583 0.03356234 0.2325466 3.411170 0.07115417 0.03633333 0.07433333 0.03800000 0.12985910 0.02731819    
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000116266 E001 1.6317192 0.0150760006 8.311569e-01 8.941673e-01 1 108746674 108746681 8 + 0.334 0.393 0.346
ENSG00000116266 E002 72.7683165 0.0003712518 1.493123e-02 4.033187e-02 1 108746682 108746786 105 + 1.782 1.674 -0.365
ENSG00000116266 E003 78.8017677 0.0003519912 6.950072e-03 2.109339e-02 1 108752257 108752306 50 + 1.818 1.700 -0.398
ENSG00000116266 E004 76.6271323 0.0003160270 4.157590e-02 9.395380e-02 1 108753063 108753144 82 + 1.800 1.719 -0.275
ENSG00000116266 E005 0.0000000       1 108753145 108753201 57 +      
ENSG00000116266 E006 84.0061538 0.0003296293 1.043829e-01 1.971628e-01 1 108756690 108756766 77 + 1.835 1.781 -0.184
ENSG00000116266 E007 0.8920269 0.2364142606 3.642010e-01 5.085633e-01 1 108756767 108757005 239 + 0.271 0.001 -9.506
ENSG00000116266 E008 79.5155113 0.0003122770 7.925282e-03 2.359130e-02 1 108758510 108758588 79 + 1.821 1.706 -0.386
ENSG00000116266 E009 0.1515154 0.0428276285 1.000000e+00   1 108759057 108759223 167 + 0.059 0.001 -6.919
ENSG00000116266 E010 95.8713582 0.0003426808 9.950638e-03 2.863921e-02 1 108759985 108760052 68 + 1.898 1.802 -0.326
ENSG00000116266 E011 82.2289013 0.0039596847 4.825324e-02 1.060835e-01 1 108760053 108760085 33 + 1.832 1.743 -0.299
ENSG00000116266 E012 140.0600282 0.0016085120 2.999690e-02 7.195360e-02 1 108772665 108772819 155 + 2.056 1.991 -0.215
ENSG00000116266 E013 115.8565894 0.0003123592 1.549971e-01 2.684145e-01 1 108776333 108776423 91 + 1.968 1.935 -0.111
ENSG00000116266 E014 120.3653816 0.0002720246 2.705519e-02 6.610634e-02 1 108779286 108779410 125 + 1.990 1.924 -0.222
ENSG00000116266 E015 49.3934067 0.0004767791 3.096635e-02 7.384982e-02 1 108779411 108781542 2132 + 1.621 1.502 -0.404
ENSG00000116266 E016 19.3415749 0.0016890031 3.368295e-01 4.806952e-01 1 108781861 108782421 561 + 1.224 1.152 -0.255
ENSG00000116266 E017 118.0565541 0.0002428837 1.531858e-02 4.121115e-02 1 108782422 108782517 96 + 1.984 1.908 -0.257
ENSG00000116266 E018 94.4516547 0.0003092612 7.968396e-01 8.703604e-01 1 108782649 108782706 58 + 1.872 1.888 0.053
ENSG00000116266 E019 92.3954759 0.0003398056 8.579453e-02 1.686643e-01 1 108793582 108793647 66 + 1.877 1.821 -0.186
ENSG00000116266 E020 102.9184881 0.0011544188 1.968000e-01 3.222456e-01 1 108794827 108794907 81 + 1.918 1.884 -0.113
ENSG00000116266 E021 67.9440362 0.0030607589 4.015786e-01 5.452838e-01 1 108796234 108796234 1 + 1.737 1.714 -0.078
ENSG00000116266 E022 132.6563847 0.0031176253 8.471588e-01 9.050709e-01 1 108796235 108796372 138 + 2.014 2.050 0.122
ENSG00000116266 E023 3.8354086 0.0040772128 7.330774e-01 8.243803e-01 1 108796373 108796619 247 + 0.590 0.669 0.343
ENSG00000116266 E024 127.5290792 0.0003822613 9.700338e-01 9.852436e-01 1 108796620 108796726 107 + 1.999 2.025 0.086
ENSG00000116266 E025 137.0748695 0.0002511082 1.367450e-01 2.435501e-01 1 108798145 108798237 93 + 2.017 2.099 0.276
ENSG00000116266 E026 119.3596076 0.0002446389 1.990613e-03 7.197827e-03 1 108800220 108800305 86 + 1.943 2.089 0.490
ENSG00000116266 E027 158.7331310 0.0002399503 9.983991e-03 2.872429e-02 1 108807401 108807549 149 + 2.074 2.188 0.382
ENSG00000116266 E028 246.2141907 0.0089451789 7.813362e-11 1.403849e-09 1 108808783 108809523 741 + 2.201 2.540 1.131