ENSG00000116221

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000360840 ENSG00000116221 HEK293_OSMI2_2hA HEK293_TMG_2hB MRPL37 protein_coding protein_coding 450.6209 829.8561 266.0587 21.3334 2.089973 -1.64108 412.20393 741.88269 243.3519 51.01215 3.053818 -1.608107 0.91870417 0.89200000 0.9146 0.02260000 9.169041e-01 8.661766e-11 FALSE  
MSTRG.1246.6 ENSG00000116221 HEK293_OSMI2_2hA HEK293_TMG_2hB MRPL37 protein_coding   450.6209 829.8561 266.0587 21.3334 2.089973 -1.64108 14.18314 53.26011 0.0000 29.35722 0.000000 -12.379111 0.01996667 0.06603333 0.0000 -0.06603333 8.661766e-11 8.661766e-11 FALSE  
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000116221 E001 0.1817044 3.924771e-02 1.000000e+00   1 54184041 54184300 260 + 0.001 0.071 7.016
ENSG00000116221 E002 1.2386081 3.994223e-01 7.570732e-01 8.420236e-01 1 54200143 54200148 6 + 0.341 0.281 -0.386
ENSG00000116221 E003 7.1170047 2.447765e-03 2.890579e-01 4.297307e-01 1 54200149 54200166 18 + 0.912 0.788 -0.480
ENSG00000116221 E004 16.4231443 4.450653e-02 3.968518e-01 5.406121e-01 1 54200167 54200178 12 + 1.219 1.119 -0.355
ENSG00000116221 E005 450.5134217 7.911731e-03 2.236937e-01 3.548142e-01 1 54200179 54200195 17 + 2.581 2.548 -0.110
ENSG00000116221 E006 597.0069821 7.334119e-03 3.340348e-03 1.126373e-02 1 54200196 54200203 8 + 2.760 2.653 -0.356
ENSG00000116221 E007 1514.4249781 9.465761e-03 2.527105e-01 3.891094e-01 1 54200204 54200241 38 + 3.102 3.075 -0.090
ENSG00000116221 E008 5512.0811063 2.338194e-03 2.795543e-05 1.674436e-04 1 54200242 54200589 348 + 3.695 3.628 -0.223
ENSG00000116221 E009 5669.0131947 1.042125e-04 1.771140e-46 8.829928e-44 1 54205018 54205201 184 + 3.713 3.637 -0.252
ENSG00000116221 E010 5145.2286477 3.235491e-05 8.560241e-12 1.802904e-10 1 54205295 54205410 116 + 3.628 3.606 -0.071
ENSG00000116221 E011 1.3200789 1.116974e-02 8.443173e-01 9.031097e-01 1 54206760 54206849 90 + 0.341 0.314 -0.170
ENSG00000116221 E012 1.6876418 1.785654e-01 3.595153e-01 5.038438e-01 1 54206850 54206877 28 + 0.529 0.342 -0.992
ENSG00000116221 E013 9.0854112 1.099708e-01 9.344310e-01 9.626844e-01 1 54207360 54207504 145 + 0.942 0.908 -0.126
ENSG00000116221 E014 2033.6643691 2.486823e-04 9.332419e-02 1.803149e-01 1 54209946 54209947 2 + 3.175 3.216 0.138
ENSG00000116221 E015 6459.9048022 3.588956e-04 2.523609e-02 6.235933e-02 1 54209948 54210131 184 + 3.676 3.719 0.144
ENSG00000116221 E016 8.2194846 1.501525e-02 1.180373e-01 2.171282e-01 1 54210132 54210132 1 + 0.661 0.925 1.047
ENSG00000116221 E017 6097.8824854 4.994290e-04 8.092074e-06 5.488398e-05 1 54212501 54212658 158 + 3.627 3.699 0.237
ENSG00000116221 E018 6355.5199569 1.010212e-03 1.653738e-12 3.907404e-11 1 54216141 54216344 204 + 3.599 3.726 0.420
ENSG00000116221 E019 3722.4043416 1.475402e-03 1.540370e-18 8.285591e-17 1 54218172 54218401 230 + 3.311 3.503 0.641
ENSG00000116221 E020 56.7700892 6.483041e-03 1.512119e-12 3.597469e-11 1 54220611 54220930 320 + 1.963 1.550 -1.398
ENSG00000116221 E021 203.3869858 1.404765e-03 1.521171e-27 2.082234e-25 1 54225125 54225517 393 + 2.450 2.122 -1.095