ENSG00000116127

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000484298 ENSG00000116127 HEK293_OSMI2_2hA HEK293_TMG_2hB ALMS1 protein_coding protein_coding 5.758063 4.677954 6.36338 0.2414066 0.2276182 0.4431003 1.13979874 0.176212603 2.3255272 0.148582151 0.56690327 3.648726 0.18057917 0.03826667 0.36860000 0.33033333 1.867022e-02 3.481173e-08 FALSE TRUE
ENST00000620466 ENSG00000116127 HEK293_OSMI2_2hA HEK293_TMG_2hB ALMS1 protein_coding retained_intron 5.758063 4.677954 6.36338 0.2414066 0.2276182 0.4431003 0.34950735 0.068339240 0.3681592 0.051406908 0.10931774 2.271187 0.05746250 0.01516667 0.05836667 0.04320000 2.185856e-01 3.481173e-08 FALSE TRUE
ENST00000651057 ENSG00000116127 HEK293_OSMI2_2hA HEK293_TMG_2hB ALMS1 protein_coding protein_coding 5.758063 4.677954 6.36338 0.2414066 0.2276182 0.4431003 1.53524446 2.414435745 0.2507450 0.169925561 0.25074500 -3.216938 0.28772500 0.51686667 0.04240000 -0.47446667 1.469866e-02 3.481173e-08 FALSE TRUE
ENST00000652487 ENSG00000116127 HEK293_OSMI2_2hA HEK293_TMG_2hB ALMS1 protein_coding nonsense_mediated_decay 5.758063 4.677954 6.36338 0.2414066 0.2276182 0.4431003 0.13046282 0.357370413 0.0000000 0.080712396 0.00000000 -5.199164 0.02501667 0.07823333 0.00000000 -0.07823333 9.588872e-07 3.481173e-08 TRUE TRUE
ENST00000682859 ENSG00000116127 HEK293_OSMI2_2hA HEK293_TMG_2hB ALMS1 protein_coding protein_coding 5.758063 4.677954 6.36338 0.2414066 0.2276182 0.4431003 0.30902193 0.000000000 0.3958120 0.000000000 0.39581203 5.342740 0.04555833 0.00000000 0.05950000 0.05950000 8.750147e-01 3.481173e-08 FALSE TRUE
ENST00000683147 ENSG00000116127 HEK293_OSMI2_2hA HEK293_TMG_2hB ALMS1 protein_coding retained_intron 5.758063 4.677954 6.36338 0.2414066 0.2276182 0.4431003 0.34952372 0.152417912 0.5224878 0.021638677 0.08740701 1.713038 0.06147500 0.03230000 0.08140000 0.04910000 6.388626e-02 3.481173e-08   FALSE
ENST00000684460 ENSG00000116127 HEK293_OSMI2_2hA HEK293_TMG_2hB ALMS1 protein_coding protein_coding 5.758063 4.677954 6.36338 0.2414066 0.2276182 0.4431003 0.29186054 0.152242642 0.3618571 0.042904294 0.09110766 1.196595 0.05239167 0.03356667 0.05736667 0.02380000 5.401293e-01 3.481173e-08 FALSE TRUE
ENST00000684590 ENSG00000116127 HEK293_OSMI2_2hA HEK293_TMG_2hB ALMS1 protein_coding protein_coding 5.758063 4.677954 6.36338 0.2414066 0.2276182 0.4431003 0.25197360 0.425815329 0.0000000 0.074724957 0.00000000 -5.445645 0.05260833 0.09246667 0.00000000 -0.09246667 3.481173e-08 3.481173e-08 FALSE TRUE
MSTRG.18664.20 ENSG00000116127 HEK293_OSMI2_2hA HEK293_TMG_2hB ALMS1 protein_coding   5.758063 4.677954 6.36338 0.2414066 0.2276182 0.4431003 0.08279166 0.009499128 0.3281859 0.009499128 0.16423567 4.116335 0.01513750 0.00220000 0.05296667 0.05076667 3.422391e-01 3.481173e-08 TRUE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000116127 E001 0.0000000       2 73385758 73385835 78 +      
ENSG00000116127 E002 0.0000000       2 73385836 73385868 33 +      
ENSG00000116127 E003 0.0000000       2 73385869 73385903 35 +      
ENSG00000116127 E004 0.0000000       2 73385904 73385908 5 +      
ENSG00000116127 E005 0.7750806 0.0230784363 4.720859e-01 6.111429e-01 2 73385909 73385996 88 + 0.300 0.154 -1.224
ENSG00000116127 E006 0.7750806 0.0230784363 4.720859e-01 6.111429e-01 2 73385997 73385997 1 + 0.300 0.154 -1.224
ENSG00000116127 E007 8.4436866 0.0039017055 6.214434e-06 4.330559e-05 2 73385998 73386192 195 + 1.144 0.495 -2.603
ENSG00000116127 E008 0.1451727 0.0429168873 9.037183e-01   2 73408622 73408747 126 + 0.096 0.000 -10.305
ENSG00000116127 E009 15.3517499 0.0011537758 2.063233e-12 4.808973e-11 2 73419123 73419318 196 + 1.400 0.551 -3.239
ENSG00000116127 E010 1.1655342 0.3532797463 8.655262e-02 1.698886e-01 2 73419319 73422701 3383 + 0.473 0.000 -12.725
ENSG00000116127 E011 13.2860869 0.0141753298 3.752777e-12 8.378932e-11 2 73422857 73422974 118 + 1.355 0.268 -4.665
ENSG00000116127 E012 27.8046251 0.0007188460 2.578709e-19 1.525222e-17 2 73424430 73424902 473 + 1.644 0.815 -2.958
ENSG00000116127 E013 0.0000000       2 73424903 73424973 71 +      
ENSG00000116127 E014 11.5670642 0.0014517523 1.576613e-07 1.541493e-06 2 73426453 73426553 101 + 1.265 0.600 -2.546
ENSG00000116127 E015 0.4513240 0.1676728802 1.000000e+00 1.000000e+00 2 73432129 73432197 69 + 0.176 0.153 -0.241
ENSG00000116127 E016 10.6219681 0.0096307323 8.013575e-04 3.264343e-03 2 73432198 73432291 94 + 1.195 0.754 -1.650
ENSG00000116127 E017 0.0000000       2 73432513 73432685 173 +      
ENSG00000116127 E018 13.7357570 0.0109815229 6.871898e-06 4.741280e-05 2 73447960 73448090 131 + 1.320 0.754 -2.090
ENSG00000116127 E019 64.4986272 0.0502171932 5.624910e-09 7.275860e-08 2 73448091 73449456 1366 + 1.990 1.245 -2.544
ENSG00000116127 E020 40.6040098 0.0747997645 2.574429e-05 1.556066e-04 2 73449457 73450280 824 + 1.779 1.143 -2.196
ENSG00000116127 E021 20.4453606 0.0979312668 4.453976e-04 1.949072e-03 2 73450281 73450565 285 + 1.489 0.871 -2.215
ENSG00000116127 E022 55.9434193 0.0372845988 2.178220e-06 1.680201e-05 2 73450566 73451558 993 + 1.901 1.348 -1.884
ENSG00000116127 E023 15.6116965 0.0668502230 1.172636e-02 3.288007e-02 2 73451559 73451696 138 + 1.340 0.922 -1.508
ENSG00000116127 E024 45.8223912 0.0029344427 5.529803e-14 1.630601e-12 2 73451697 73452410 714 + 1.814 1.277 -1.841
ENSG00000116127 E025 28.2476490 0.0228498468 1.137732e-04 5.851023e-04 2 73452411 73452724 314 + 1.588 1.145 -1.542
ENSG00000116127 E026 42.9326170 0.0005583376 1.007141e-06 8.348840e-06 2 73452725 73453306 582 + 1.740 1.417 -1.102
ENSG00000116127 E027 55.6876453 0.0037085575 1.285032e-06 1.042593e-05 2 73453307 73454067 761 + 1.851 1.530 -1.088
ENSG00000116127 E028 13.6857472 0.0238164626 2.498782e-01 3.857549e-01 2 73455162 73455212 51 + 1.222 1.051 -0.612
ENSG00000116127 E029 17.2244556 0.0302446045 9.171817e-02 1.778132e-01 2 73455213 73455295 83 + 1.335 1.099 -0.835
ENSG00000116127 E030 1.1929815 0.0130214520 1.761812e-02 4.630870e-02 2 73455296 73455910 615 + 0.476 0.000 -13.181
ENSG00000116127 E031 46.6155890 0.0200626297 1.993638e-02 5.135407e-02 2 73489634 73490158 525 + 1.754 1.526 -0.774
ENSG00000116127 E032 59.7890247 0.0132431570 6.156534e-01 7.338732e-01 2 73490159 73490862 704 + 1.804 1.735 -0.235
ENSG00000116127 E033 62.8905062 0.0079927677 8.553515e-01 9.105743e-01 2 73490863 73491498 636 + 1.806 1.782 -0.080
ENSG00000116127 E034 32.6027632 0.0119915531 2.421044e-01 3.766822e-01 2 73519775 73519955 181 + 1.493 1.556 0.217
ENSG00000116127 E035 16.3836538 0.0011342130 5.479530e-01 6.778966e-01 2 73519956 73520016 61 + 1.228 1.246 0.061
ENSG00000116127 E036 30.0819169 0.0006787445 6.238876e-01 7.403655e-01 2 73534824 73534949 126 + 1.486 1.483 -0.010
ENSG00000116127 E037 33.2939481 0.0006654107 9.166820e-01 9.513468e-01 2 73550267 73550437 171 + 1.542 1.501 -0.141
ENSG00000116127 E038 24.1617665 0.0007719602 7.812182e-02 1.564575e-01 2 73557220 73557354 135 + 1.355 1.445 0.311
ENSG00000116127 E039 31.1815905 0.0006633689 6.137030e-03 1.897045e-02 2 73558972 73559142 171 + 1.445 1.580 0.464
ENSG00000116127 E040 23.1980946 0.0008687651 4.921695e-03 1.571205e-02 2 73572262 73572310 49 + 1.304 1.470 0.575
ENSG00000116127 E041 46.3190686 0.0004713114 1.406124e-02 3.836314e-02 2 73572311 73572489 179 + 1.629 1.720 0.311
ENSG00000116127 E042 130.0333012 0.0007427642 6.565020e-06 4.549597e-05 2 73572490 73573309 820 + 2.064 2.173 0.366
ENSG00000116127 E043 43.0506730 0.0005737030 4.582233e-05 2.606251e-04 2 73573310 73573424 115 + 1.548 1.731 0.623
ENSG00000116127 E044 0.9901831 0.0845559723 9.551423e-01 9.758495e-01 2 73573425 73573580 156 + 0.300 0.269 -0.213
ENSG00000116127 E045 0.3332198 0.0282973579 6.505920e-01   2 73593749 73593887 139 + 0.096 0.154 0.776
ENSG00000116127 E046 0.1472490 0.0427432632 9.038432e-01   2 73599244 73599367 124 + 0.096 0.000 -10.305
ENSG00000116127 E047 29.6142745 0.0008390370 8.237691e-03 2.438713e-02 2 73599401 73599450 50 + 1.421 1.555 0.461
ENSG00000116127 E048 37.4078921 0.0035101675 1.223716e-02 3.410307e-02 2 73599451 73599521 71 + 1.523 1.644 0.414
ENSG00000116127 E049 51.9342120 0.0008591957 2.901094e-07 2.693475e-06 2 73600678 73600834 157 + 1.610 1.829 0.741
ENSG00000116127 E050 32.9470649 0.0006330991 3.491658e-07 3.186730e-06 2 73600835 73600881 47 + 1.391 1.664 0.934
ENSG00000116127 E051 62.8474526 0.0006808102 1.566727e-17 7.476367e-16 2 73601195 73601436 242 + 1.624 1.970 1.171
ENSG00000116127 E052 57.2105041 0.0082153711 4.726061e-10 7.429575e-09 2 73602185 73602368 184 + 1.577 1.932 1.199
ENSG00000116127 E053 36.9668583 0.0190606072 1.026853e-06 8.495317e-06 2 73603241 73603298 58 + 1.369 1.764 1.352
ENSG00000116127 E054 27.9771285 0.0153733272 2.166911e-07 2.063303e-06 2 73603299 73603304 6 + 1.234 1.657 1.457
ENSG00000116127 E055 2.6059972 0.0735892159 6.295684e-02 1.316386e-01 2 73603305 73603859 555 + 0.674 0.269 -2.119
ENSG00000116127 E056 1.3369065 0.3366673410 8.730833e-01 9.224313e-01 2 73603931 73603991 61 + 0.397 0.347 -0.291
ENSG00000116127 E057 2.8579109 0.0061718986 6.479283e-02 1.347030e-01 2 73605034 73605135 102 + 0.697 0.357 -1.640
ENSG00000116127 E058 3.5062277 0.0385340108 9.856482e-02 1.883023e-01 2 73605136 73605213 78 + 0.758 0.432 -1.473
ENSG00000116127 E059 26.4405484 0.0083081930 6.061985e-02 1.276751e-01 2 73605214 73608370 3157 + 1.503 1.313 -0.659
ENSG00000116127 E060 3.1137097 0.0062027314 9.991067e-01 1.000000e+00 2 73608371 73608474 104 + 0.627 0.600 -0.118
ENSG00000116127 E061 45.5997687 0.0152632492 2.034949e-08 2.369484e-07 2 73608475 73608574 100 + 1.449 1.855 1.381
ENSG00000116127 E062 21.5650300 0.0398563261 6.630826e-05 3.622441e-04 2 73609568 73609569 2 + 1.104 1.556 1.581
ENSG00000116127 E063 35.4749883 0.0131810810 5.050891e-08 5.441124e-07 2 73609570 73609682 113 + 1.345 1.744 1.366
ENSG00000116127 E064 14.7787377 0.0012050171 3.167037e-09 4.278278e-08 2 73609683 73609916 234 + 0.927 1.417 1.751
ENSG00000116127 E065 1.4101723 0.0102208255 9.175198e-01 9.518842e-01 2 73610437 73610793 357 + 0.397 0.357 -0.225
ENSG00000116127 E066 0.8877725 0.0144517209 9.306657e-01 9.603668e-01 2 73615267 73615380 114 + 0.300 0.267 -0.226
ENSG00000116127 E067 2.2260113 0.4090873431 7.129864e-01 8.095580e-01 2 73624677 73625166 490 + 0.511 0.454 -0.283