ENSG00000116016

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000263734 ENSG00000116016 HEK293_OSMI2_2hA HEK293_TMG_2hB EPAS1 protein_coding protein_coding 3.388814 4.843861 2.912182 0.1549416 0.3898744 -0.7320867 2.31857598 2.322726 2.4231005 0.2301350 0.04525303 0.0607793 0.7537250 0.4833333 0.8630 0.3796667 6.694844e-02 5.176933e-15 FALSE TRUE
ENST00000463191 ENSG00000116016 HEK293_OSMI2_2hA HEK293_TMG_2hB EPAS1 protein_coding retained_intron 3.388814 4.843861 2.912182 0.1549416 0.3898744 -0.7320867 0.04669359 0.000000 0.3735488 0.0000000 0.37354876 5.2613381 0.0126500 0.0000000 0.1012 0.1012000 7.369193e-01 5.176933e-15 FALSE FALSE
ENST00000468530 ENSG00000116016 HEK293_OSMI2_2hA HEK293_TMG_2hB EPAS1 protein_coding processed_transcript 3.388814 4.843861 2.912182 0.1549416 0.3898744 -0.7320867 0.91344124 2.450041 0.0000000 0.3923441 0.00000000 -7.9425388 0.1976208 0.5016667 0.0000 -0.5016667 5.176933e-15 5.176933e-15 FALSE FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000116016 E001 0.0000000       2 46293667 46294098 432 +      
ENSG00000116016 E002 0.0000000       2 46296608 46296725 118 +      
ENSG00000116016 E003 13.4402266 0.0154413144 3.528459e-01 4.970968e-01 2 46297407 46297741 335 + 1.160 1.135 -0.088
ENSG00000116016 E004 5.5379073 0.0030013847 4.154321e-01 5.588165e-01 2 46297742 46297763 22 + 0.824 0.784 -0.155
ENSG00000116016 E005 11.6094532 0.0028310490 1.846188e-02 4.814575e-02 2 46297764 46297937 174 + 1.170 1.012 -0.572
ENSG00000116016 E006 0.0000000       2 46335690 46335906 217 +      
ENSG00000116016 E007 19.0981560 0.0010151886 5.670140e-04 2.410175e-03 2 46346873 46347063 191 + 1.393 1.203 -0.665
ENSG00000116016 E008 0.0000000       2 46347238 46347537 300 +      
ENSG00000116016 E009 0.0000000       2 46356115 46356150 36 +      
ENSG00000116016 E010 22.1799853 0.0009261431 6.634031e-03 2.027387e-02 2 46356151 46356302 152 + 1.423 1.304 -0.413
ENSG00000116016 E011 18.8327377 0.0014942638 2.743922e-02 6.688556e-02 2 46356724 46356808 85 + 1.340 1.242 -0.344
ENSG00000116016 E012 19.7461973 0.0026206834 6.385738e-03 1.962556e-02 2 46360638 46360756 119 + 1.381 1.242 -0.483
ENSG00000116016 E013 29.6110880 0.0193859955 2.392166e-01 3.732037e-01 2 46360885 46361090 206 + 1.498 1.467 -0.107
ENSG00000116016 E014 21.1422722 0.0062024207 8.599080e-02 1.689749e-01 2 46369827 46369933 107 + 1.374 1.311 -0.221
ENSG00000116016 E015 0.0000000       2 46375636 46375689 54 +      
ENSG00000116016 E016 22.7202557 0.0008144677 3.578811e-03 1.195168e-02 2 46375690 46375837 148 + 1.440 1.311 -0.450
ENSG00000116016 E017 25.1618703 0.0063277824 2.350706e-02 5.878998e-02 2 46376539 46376753 215 + 1.462 1.365 -0.334
ENSG00000116016 E018 15.9109040 0.0092648445 3.916510e-02 8.948466e-02 2 46377894 46378087 194 + 1.288 1.169 -0.420
ENSG00000116016 E019 16.5887042 0.0018238800 6.892170e-02 1.415854e-01 2 46378657 46378767 111 + 1.280 1.203 -0.271
ENSG00000116016 E020 0.7739347 0.0148427054 4.066447e-01 5.502765e-01 2 46379700 46380226 527 + 0.314 0.204 -0.829
ENSG00000116016 E021 44.0093722 0.0005259098 1.893472e-01 3.129089e-01 2 46380227 46380717 491 + 1.638 1.647 0.030
ENSG00000116016 E022 0.6362506 0.4713216240 3.912839e-01 5.352074e-01 2 46381177 46381595 419 + 0.316 0.114 -1.832
ENSG00000116016 E023 14.2501631 0.0013471270 6.610357e-01 7.695787e-01 2 46381596 46381634 39 + 1.160 1.195 0.123
ENSG00000116016 E024 21.1037983 0.0010496876 2.683321e-01 4.067785e-01 2 46381635 46381722 88 + 1.347 1.341 -0.022
ENSG00000116016 E025 24.5540917 0.0008617289 4.838348e-01 6.218585e-01 2 46381975 46382089 115 + 1.380 1.407 0.092
ENSG00000116016 E026 31.3195909 0.0007500384 7.261900e-01 8.192763e-01 2 46382425 46382598 174 + 1.440 1.538 0.336
ENSG00000116016 E027 0.0000000       2 46384476 46384508 33 +      
ENSG00000116016 E028 252.0662406 0.0111452145 4.507716e-09 5.924610e-08 2 46384509 46386697 2189 + 2.192 2.497 1.018