ENSG00000115966

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000264110 ENSG00000115966 HEK293_OSMI2_2hA HEK293_TMG_2hB ATF2 protein_coding protein_coding 12.50846 2.403136 21.6216 0.3039014 0.8306393 3.164159 5.1733838 0.932170 8.418550 0.2754569 0.51234320 3.161225 0.36537917 0.3771667 0.38883333 0.01166667 9.707696e-01 1.433295e-08 FALSE TRUE
ENST00000345739 ENSG00000115966 HEK293_OSMI2_2hA HEK293_TMG_2hB ATF2 protein_coding protein_coding 12.50846 2.403136 21.6216 0.3039014 0.8306393 3.164159 1.9151669 1.128094 2.963402 0.2307708 0.05054637 1.385495 0.27544583 0.4752333 0.13746667 -0.33776667 1.568934e-04 1.433295e-08 FALSE TRUE
ENST00000392544 ENSG00000115966 HEK293_OSMI2_2hA HEK293_TMG_2hB ATF2 protein_coding protein_coding 12.50846 2.403136 21.6216 0.3039014 0.8306393 3.164159 0.9440442 0.000000 3.343180 0.0000000 0.51301818 8.389386 0.06058750 0.0000000 0.15390000 0.15390000 1.433295e-08 1.433295e-08 FALSE TRUE
ENST00000456655 ENSG00000115966 HEK293_OSMI2_2hA HEK293_TMG_2hB ATF2 protein_coding nonsense_mediated_decay 12.50846 2.403136 21.6216 0.3039014 0.8306393 3.164159 2.0741656 0.000000 1.713040 0.0000000 0.89464072 7.428812 0.10062083 0.0000000 0.08133333 0.08133333 4.811044e-01 1.433295e-08 TRUE TRUE
ENST00000612082 ENSG00000115966 HEK293_OSMI2_2hA HEK293_TMG_2hB ATF2 protein_coding misc_RNA 12.50846 2.403136 21.6216 0.3039014 0.8306393 3.164159 0.7338963 0.000000 1.942011 0.0000000 0.18031470 7.608817 0.04634583 0.0000000 0.08990000 0.08990000 1.467135e-06 1.433295e-08   FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000115966 E001 0.4448795 0.3408073170 9.415527e-03 0.0273207338 2 175072250 175072253 4 - 0.000 0.543 14.195
ENSG00000115966 E002 0.5921286 0.2378825677 2.379446e-02 0.0593903680 2 175072254 175072258 5 - 0.050 0.546 4.370
ENSG00000115966 E003 1.3972964 0.0330551027 3.691166e-05 0.0002145295 2 175072259 175072264 6 - 0.095 0.847 4.629
ENSG00000115966 E004 330.0392440 0.0253864601 6.711258e-03 0.0204732155 2 175072265 175074279 2015 - 2.323 2.570 0.824
ENSG00000115966 E005 31.8765240 0.0019386759 4.975292e-01 0.6340761183 2 175074280 175074391 112 - 1.374 1.372 -0.006
ENSG00000115966 E006 18.2705511 0.0014074535 9.384565e-06 0.0000627826 2 175074392 175074473 82 - 1.041 1.497 1.605
ENSG00000115966 E007 18.1912572 0.0081065919 6.410422e-05 0.0003515415 2 175074474 175074504 31 - 1.041 1.485 1.563
ENSG00000115966 E008 112.3847841 0.0002642737 9.621251e-01 0.9801030227 2 175074505 175074835 331 - 1.895 1.957 0.207
ENSG00000115966 E009 2.2800636 0.0069600481 9.541808e-01 0.9752846017 2 175074836 175074867 32 - 0.412 0.437 0.133
ENSG00000115966 E010 3.4926118 0.0172114122 9.162349e-01 0.9510443251 2 175075000 175075087 88 - 0.536 0.557 0.098
ENSG00000115966 E011 4.3269565 0.1012429371 1.055567e-01 0.1989414968 2 175080263 175080659 397 - 0.646 0.270 -1.989
ENSG00000115966 E012 65.0998440 0.0004171213 2.878711e-01 0.4284149102 2 175080660 175080765 106 - 1.671 1.664 -0.021
ENSG00000115966 E013 2.2143871 0.0065393392 3.817324e-01 0.5260447972 2 175092401 175092542 142 - 0.432 0.271 -0.974
ENSG00000115966 E014 4.6379379 0.0131274123 7.434387e-01 0.8320665293 2 175092543 175092616 74 - 0.620 0.727 0.450
ENSG00000115966 E015 105.3162819 0.0002764900 2.973158e-01 0.4386352033 2 175093061 175093267 207 - 1.874 1.884 0.033
ENSG00000115966 E016 84.1113503 0.0022290153 5.994708e-01 0.7208420285 2 175097444 175097593 150 - 1.775 1.803 0.095
ENSG00000115966 E017 60.1492719 0.0003791794 9.049389e-01 0.9434883419 2 175111568 175111646 79 - 1.629 1.680 0.175
ENSG00000115966 E018 40.4679496 0.0005925824 3.729693e-01 0.5174016399 2 175111647 175111654 8 - 1.470 1.459 -0.037
ENSG00000115966 E019 69.9516832 0.0003515121 1.536653e-02 0.0413186008 2 175113994 175114108 115 - 1.713 1.622 -0.309
ENSG00000115966 E020 0.3030308 0.3711596421 1.000000e+00   2 175114243 175114689 447 - 0.095 0.000 -8.403
ENSG00000115966 E021 75.6903593 0.0003673514 5.355078e-03 0.0168974063 2 175114690 175114817 128 - 1.748 1.639 -0.369
ENSG00000115966 E022 50.2807356 0.0004531697 2.946094e-02 0.0708954151 2 175114818 175114868 51 - 1.573 1.472 -0.346
ENSG00000115966 E023 71.0901216 0.0029840895 4.589549e-02 0.1018480002 2 175117990 175118118 129 - 1.717 1.640 -0.259
ENSG00000115966 E024 71.0944962 0.0055031046 3.894120e-03 0.0128468468 2 175118251 175118369 119 - 1.729 1.573 -0.530
ENSG00000115966 E025 46.6857934 0.0010629279 2.934059e-03 0.0100695389 2 175121444 175121540 97 - 1.553 1.373 -0.621
ENSG00000115966 E026 1.2576127 0.0102906281 4.079481e-01 0.5515439365 2 175126793 175126992 200 - 0.238 0.437 1.249
ENSG00000115966 E027 0.8094091 0.0160944345 1.521927e-01 0.2646684805 2 175126993 175127058 66 - 0.135 0.437 2.249
ENSG00000115966 E028 1.6284970 0.0092734394 1.924800e-01 0.3168772623 2 175127156 175127231 76 - 0.268 0.557 1.612
ENSG00000115966 E029 60.4019240 0.0004435403 6.025114e-02 0.1270369523 2 175130138 175130207 70 - 1.649 1.584 -0.220
ENSG00000115966 E030 0.0000000       2 175132695 175132757 63 -      
ENSG00000115966 E031 52.1329952 0.0007258740 3.634733e-01 0.5078765943 2 175136412 175136486 75 - 1.578 1.574 -0.013
ENSG00000115966 E032 0.9493850 0.2084171530 7.798665e-01 0.8585030569 2 175147946 175147980 35 - 0.206 0.274 0.538
ENSG00000115966 E033 65.6080635 0.0042824693 6.791377e-02 0.1399077911 2 175151060 175151158 99 - 1.684 1.613 -0.242
ENSG00000115966 E034 50.6313481 0.0063046402 1.160231e-02 0.0325884033 2 175168050 175168206 157 - 1.586 1.431 -0.529
ENSG00000115966 E035 0.8815316 0.0136968632 4.215371e-01 0.5646774673 2 175168286 175168382 97 - 0.238 0.000 -9.980