ENSG00000115866

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000264161 ENSG00000115866 HEK293_OSMI2_2hA HEK293_TMG_2hB DARS1 protein_coding protein_coding 70.96474 21.89137 120.8906 2.760076 2.163475 2.464728 28.963213 14.18113880 49.188159 1.37662566 0.883569 1.793614 0.50036250 0.653100000 0.40710000 -0.2460000 8.301391e-10 5.59839e-54 FALSE TRUE
ENST00000422708 ENSG00000115866 HEK293_OSMI2_2hA HEK293_TMG_2hB DARS1 protein_coding protein_coding 70.96474 21.89137 120.8906 2.760076 2.163475 2.464728 19.713222 7.49862549 32.445350 1.39527350 1.705811 2.111836 0.28545833 0.336900000 0.26810000 -0.0688000 1.788139e-01 5.59839e-54 FALSE TRUE
ENST00000435076 ENSG00000115866 HEK293_OSMI2_2hA HEK293_TMG_2hB DARS1 protein_coding nonsense_mediated_decay 70.96474 21.89137 120.8906 2.760076 2.163475 2.464728 4.216717 0.06893871 7.654795 0.06893871 1.683281 6.601370 0.03418333 0.004033333 0.06383333 0.0598000 1.102570e-02 5.59839e-54 FALSE TRUE
ENST00000441323 ENSG00000115866 HEK293_OSMI2_2hA HEK293_TMG_2hB DARS1 protein_coding protein_coding 70.96474 21.89137 120.8906 2.760076 2.163475 2.464728 16.921560 0.00000000 30.370312 0.00000000 2.141272 11.568921 0.15643750 0.000000000 0.25076667 0.2507667 5.598390e-54 5.59839e-54 FALSE FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000115866 E001 191.2413418 0.0005928820 4.957466e-25 5.438200e-23 2 135905881 135906823 943 - 2.129 2.433 1.012
ENSG00000115866 E002 438.1262334 0.0001802394 2.298199e-61 2.473789e-58 2 135906824 135907243 420 - 2.487 2.794 1.024
ENSG00000115866 E003 35.6239486 0.0011110997 6.408521e-12 1.376502e-10 2 135907244 135907259 16 - 1.359 1.785 1.455
ENSG00000115866 E004 273.2863794 0.0017524555 1.883729e-06 1.474230e-05 2 135907260 135907407 148 - 2.339 2.494 0.515
ENSG00000115866 E005 1.1887270 0.0116987063 8.830054e-01 9.291278e-01 2 135910848 135911138 291 - 0.312 0.344 0.206
ENSG00000115866 E006 327.3766873 0.0043493956 5.339841e-02 1.152026e-01 2 135911139 135911210 72 - 2.438 2.522 0.278
ENSG00000115866 E007 462.5327853 0.0003243289 9.979831e-03 2.871450e-02 2 135911382 135911493 112 - 2.595 2.653 0.192
ENSG00000115866 E008 0.8189741 0.0305920844 9.283460e-01 9.589133e-01 2 135911494 135911617 124 - 0.230 0.206 -0.204
ENSG00000115866 E009 386.6479122 0.0001601488 1.274955e-04 6.477936e-04 2 135912486 135912566 81 - 2.510 2.595 0.283
ENSG00000115866 E010 324.7641142 0.0011093919 1.247604e-05 8.109120e-05 2 135914469 135914511 43 - 2.423 2.546 0.408
ENSG00000115866 E011 2.8378386 0.0051672003 1.734847e-01 2.927238e-01 2 135914512 135914866 355 - 0.599 0.345 -1.294
ENSG00000115866 E012 576.8055373 0.0001345723 7.256231e-02 1.476141e-01 2 135916226 135916372 147 - 2.697 2.734 0.124
ENSG00000115866 E013 544.6565124 0.0001625494 1.300933e-02 3.593288e-02 2 135920453 135920600 148 - 2.690 2.651 -0.130
ENSG00000115866 E014 426.4181568 0.0004779736 1.778879e-02 4.668281e-02 2 135922784 135922918 135 - 2.585 2.537 -0.159
ENSG00000115866 E015 230.2096787 0.0008918735 7.382827e-01 8.281942e-01 2 135924387 135924417 31 - 2.308 2.304 -0.013
ENSG00000115866 E016 198.0661911 0.0013884347 2.063706e-01 3.341620e-01 2 135924418 135924436 19 - 2.252 2.214 -0.126
ENSG00000115866 E017 307.0540974 0.0001921274 3.076636e-03 1.049195e-02 2 135924437 135924498 62 - 2.447 2.382 -0.219
ENSG00000115866 E018 346.3821669 0.0001708881 8.081579e-07 6.845043e-06 2 135932783 135932842 60 - 2.509 2.401 -0.361
ENSG00000115866 E019 0.8751888 0.1453650046 2.194036e-01 3.496648e-01 2 135933909 135933909 1 - 0.311 0.000 -11.522
ENSG00000115866 E020 322.4344105 0.0001613439 1.602787e-06 1.273137e-05 2 135933910 135933990 81 - 2.478 2.370 -0.363
ENSG00000115866 E021 0.1817044 0.0474250425 1.288838e-01   2 135943179 135943377 199 - 0.000 0.206 12.253
ENSG00000115866 E022 161.3536826 0.0002430027 1.433841e-04 7.182741e-04 2 135943378 135943385 8 - 2.183 2.060 -0.412
ENSG00000115866 E023 379.6200288 0.0001423645 4.537629e-09 5.961030e-08 2 135943386 135943480 95 - 2.553 2.429 -0.413
ENSG00000115866 E024 399.1782907 0.0001502520 3.601874e-10 5.787162e-09 2 135961396 135961498 103 - 2.575 2.445 -0.436
ENSG00000115866 E025 1.6210229 0.0972251855 7.983459e-01 8.712978e-01 2 135978987 135979273 287 - 0.380 0.445 0.357
ENSG00000115866 E026 387.6303754 0.0001586371 7.105833e-12 1.515796e-10 2 135979274 135979366 93 - 2.566 2.419 -0.490
ENSG00000115866 E027 0.0000000       2 135980431 135980551 121 -      
ENSG00000115866 E028 291.3740301 0.0002041143 1.249890e-13 3.503297e-12 2 135983397 135983454 58 - 2.452 2.263 -0.630
ENSG00000115866 E029 284.6543952 0.0007430017 5.488758e-12 1.191644e-10 2 135985403 135985626 224 - 2.443 2.248 -0.651
ENSG00000115866 E030 0.1472490 0.0438476749 1.000000e+00   2 135985794 135985812 19 - 0.070 0.000 -8.941
ENSG00000115866 E031 0.2924217 0.0271823635 9.302990e-01   2 135985813 135985897 85 - 0.130 0.000 -9.941
ENSG00000115866 E032 0.2903454 0.3448092721 1.000000e+00   2 135985930 135986100 171 - 0.130 0.000 -9.938