ENSG00000115649

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000360507 ENSG00000115649 HEK293_OSMI2_2hA HEK293_TMG_2hB CNPPD1 protein_coding protein_coding 36.42536 54.70139 16.8802 1.537697 0.2801685 -1.695655 33.47305 51.1648 14.57076 1.072344 0.2379406 -1.811368 0.9076292 0.9357 0.8639 -0.0718 0.0283632 9.006366e-09 FALSE  
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000115649 E001 429.379737 0.0053971522 9.157007e-04 0.0036695241 2 219171897 219172173 277 - 2.396 2.552 0.518
ENSG00000115649 E002 956.066659 0.0018920793 5.836627e-05 0.0003233583 2 219172174 219172893 720 - 2.783 2.891 0.363
ENSG00000115649 E003 246.010141 0.0007632224 3.658288e-01 0.5101381367 2 219172894 219173050 157 - 2.240 2.290 0.167
ENSG00000115649 E004 220.836512 0.0006380699 6.237716e-01 0.7402615454 2 219173051 219173128 78 - 2.203 2.240 0.125
ENSG00000115649 E005 290.561100 0.0005741957 7.620452e-01 0.8457450918 2 219173350 219173467 118 - 2.341 2.355 0.045
ENSG00000115649 E006 220.521115 0.0001837286 1.257658e-01 0.2282375983 2 219174146 219174207 62 - 2.253 2.231 -0.077
ENSG00000115649 E007 320.204240 0.0001727327 5.837933e-05 0.0003234140 2 219174778 219174906 129 - 2.453 2.381 -0.242
ENSG00000115649 E008 4.815155 0.0090800809 1.678363e-04 0.0008258722 2 219174907 219174987 81 - 1.036 0.532 -2.040
ENSG00000115649 E009 291.136179 0.0009667519 6.209932e-04 0.0026099901 2 219174988 219175108 121 - 2.412 2.339 -0.243
ENSG00000115649 E010 235.542106 0.0008835049 2.267482e-01 0.3585078033 2 219175591 219175672 82 - 2.274 2.259 -0.051
ENSG00000115649 E011 252.181713 0.0018973154 2.100130e-02 0.0535922221 2 219176223 219176331 109 - 2.336 2.282 -0.179
ENSG00000115649 E012 200.151187 0.0006061938 1.987901e-03 0.0071889811 2 219176760 219176847 88 - 2.252 2.177 -0.252
ENSG00000115649 E013 118.086572 0.0019770401 1.071604e-01 0.2013510075 2 219176848 219176925 78 - 2.004 1.954 -0.166
ENSG00000115649 E014 6.771842 0.0027236976 5.421451e-01 0.6729541876 2 219177104 219177313 210 - 0.855 0.789 -0.260
ENSG00000115649 E015 2.995587 0.0257387486 7.968635e-01 0.8703719441 2 219177698 219178106 409 - 0.540 0.510 -0.142
ENSG00000115649 E016 11.703861 0.0030875217 9.554570e-02 0.1837127184 2 219178121 219178353 233 - 1.125 0.958 -0.610