ENSG00000115524

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000335508 ENSG00000115524 HEK293_OSMI2_2hA HEK293_TMG_2hB SF3B1 protein_coding protein_coding 106.4757 41.30007 158.4261 4.591954 3.739834 1.939336 47.859296 12.5672231 78.409445 0.35649610 1.339937 2.640398 0.40215000 0.311233333 0.49510000 0.18386667 0.0008358293 4.858999e-07 FALSE TRUE
ENST00000409915 ENSG00000115524 HEK293_OSMI2_2hA HEK293_TMG_2hB SF3B1 protein_coding nonsense_mediated_decay 106.4757 41.30007 158.4261 4.591954 3.739834 1.939336 7.965551 0.1437065 12.851465 0.07334249 1.602834 6.386733 0.05080417 0.003866667 0.08073333 0.07686667 0.0002439106 4.858999e-07 TRUE TRUE
ENST00000424674 ENSG00000115524 HEK293_OSMI2_2hA HEK293_TMG_2hB SF3B1 protein_coding protein_coding 106.4757 41.30007 158.4261 4.591954 3.739834 1.939336 34.103356 16.6912441 48.356902 1.98780961 1.984371 1.534065 0.34567917 0.405366667 0.30543333 -0.09993333 0.1149354628 4.858999e-07 FALSE FALSE
ENST00000479532 ENSG00000115524 HEK293_OSMI2_2hA HEK293_TMG_2hB SF3B1 protein_coding retained_intron 106.4757 41.30007 158.4261 4.591954 3.739834 1.939336 8.900406 11.1890359 4.389429 2.96675902 2.206110 -1.347986 0.14491667 0.263133333 0.02800000 -0.23513333 0.2055891688 4.858999e-07 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000115524 E001 2.0745104 0.0068314233 5.546755e-01 6.836210e-01 2 197388515 197389783 1269 - 0.379 0.516 0.706
ENSG00000115524 E002 0.3697384 0.0246905512 1.282134e-02 3.549456e-02 2 197389784 197389791 8 - 0.000 0.401 13.220
ENSG00000115524 E003 747.6182454 0.0166902839 3.186498e-08 3.568858e-07 2 197389792 197392254 2463 - 2.658 3.055 1.319
ENSG00000115524 E004 730.8398335 0.0128430086 6.619469e-09 8.440157e-08 2 197392255 197392461 207 - 2.658 3.028 1.232
ENSG00000115524 E005 561.4220893 0.0049096695 4.821374e-10 7.566226e-09 2 197392972 197393051 80 - 2.574 2.849 0.915
ENSG00000115524 E006 800.0488333 0.0033992308 1.388874e-11 2.826794e-10 2 197393052 197393186 135 - 2.735 2.984 0.828
ENSG00000115524 E007 390.1935117 0.0005438356 4.626744e-20 2.957229e-18 2 197393187 197393188 2 - 2.424 2.675 0.837
ENSG00000115524 E008 3.6095806 0.0058314968 1.080277e-01 2.026149e-01 2 197393189 197393351 163 - 0.510 0.800 1.250
ENSG00000115524 E009 1062.8697168 0.0005112487 4.320849e-23 3.870317e-21 2 197396056 197396328 273 - 2.872 3.069 0.657
ENSG00000115524 E010 0.2987644 0.0273636323 1.000000e+00   2 197397954 197397984 31 - 0.107 0.000 -8.610
ENSG00000115524 E011 706.0243995 0.0028313678 2.300079e-03 8.150824e-03 2 197397985 197398116 132 - 2.711 2.838 0.424
ENSG00000115524 E012 592.2293682 0.0001447711 1.021575e-06 8.455341e-06 2 197398461 197398581 121 - 2.636 2.762 0.420
ENSG00000115524 E013 3.9620336 0.0040050659 4.677874e-01 6.072795e-01 2 197398582 197398772 191 - 0.624 0.515 -0.496
ENSG00000115524 E014 5.5900406 0.0109760527 1.432839e-01 2.524924e-01 2 197398969 197399028 60 - 0.759 0.516 -1.058
ENSG00000115524 E015 621.4434203 0.0001595185 8.403834e-03 2.479839e-02 2 197400055 197400166 112 - 2.666 2.751 0.281
ENSG00000115524 E016 710.9098466 0.0004533375 2.845994e-01 4.248438e-01 2 197400252 197400434 183 - 2.730 2.786 0.186
ENSG00000115524 E017 748.0385068 0.0006254148 9.167003e-01 9.513606e-01 2 197400715 197400936 222 - 2.756 2.788 0.107
ENSG00000115524 E018 563.1636136 0.0025392508 3.599997e-01 5.043175e-01 2 197401400 197401525 126 - 2.638 2.641 0.012
ENSG00000115524 E019 547.7572737 0.0077287630 4.180223e-01 5.613345e-01 2 197401742 197401888 147 - 2.628 2.621 -0.022
ENSG00000115524 E020 331.7268200 0.0050017417 4.446795e-02 9.923387e-02 2 197401985 197402021 37 - 2.418 2.363 -0.185
ENSG00000115524 E021 442.8073294 0.0047411433 1.661386e-01 2.831838e-01 2 197402022 197402130 109 - 2.538 2.515 -0.079
ENSG00000115524 E022 5.6970953 0.0046668134 6.064018e-01 7.265569e-01 2 197402421 197402555 135 - 0.738 0.681 -0.235
ENSG00000115524 E023 695.8053438 0.0001112563 3.920698e-07 3.540177e-06 2 197402556 197402826 271 - 2.741 2.682 -0.194
ENSG00000115524 E024 412.3432450 0.0008119840 1.389389e-04 6.989447e-04 2 197402949 197403035 87 - 2.516 2.446 -0.232
ENSG00000115524 E025 519.4779983 0.0032382817 3.593027e-04 1.616031e-03 2 197403585 197403764 180 - 2.619 2.526 -0.311
ENSG00000115524 E026 396.4075810 0.0185866515 8.060259e-02 1.604933e-01 2 197405076 197405177 102 - 2.502 2.413 -0.297
ENSG00000115524 E027 600.4399278 0.0062750074 2.981368e-04 1.371933e-03 2 197405275 197405472 198 - 2.687 2.561 -0.418
ENSG00000115524 E028 0.9027498 0.4052290482 5.239627e-01 6.576028e-01 2 197407936 197407997 62 - 0.266 0.000 -10.206
ENSG00000115524 E029 521.8939180 0.0048435929 1.160253e-07 1.165155e-06 2 197407998 197408119 122 - 2.635 2.450 -0.616
ENSG00000115524 E030 671.6518097 0.0063211911 1.045306e-07 1.057853e-06 2 197408369 197408581 213 - 2.746 2.545 -0.667
ENSG00000115524 E031 0.7415557 0.1263502670 8.541067e-01 9.097336e-01 2 197408582 197408694 113 - 0.193 0.244 0.432
ENSG00000115524 E032 645.0909274 0.0076115214 2.999867e-07 2.776232e-06 2 197409770 197410007 238 - 2.729 2.519 -0.699
ENSG00000115524 E033 0.0000000       2 197416594 197416740 147 -      
ENSG00000115524 E034 393.2771949 0.0025462838 2.883230e-09 3.926810e-08 2 197416741 197416890 150 - 2.511 2.330 -0.604
ENSG00000115524 E035 240.1679377 0.0054553033 3.666815e-07 3.332536e-06 2 197416891 197416911 21 - 2.302 2.077 -0.751
ENSG00000115524 E036 388.6808799 0.0061551996 7.397321e-07 6.322582e-06 2 197418509 197418588 80 - 2.508 2.306 -0.673
ENSG00000115524 E037 48.2496326 0.0012886278 1.538694e-05 9.794398e-05 2 197418589 197418795 207 - 1.624 1.317 -1.056
ENSG00000115524 E038 23.1251840 0.0024942959 2.110721e-04 1.011202e-03 2 197418796 197418895 100 - 1.327 0.934 -1.416
ENSG00000115524 E039 30.9408403 0.0055216424 8.714128e-05 4.616127e-04 2 197418896 197418951 56 - 1.446 1.065 -1.344
ENSG00000115524 E040 99.4762960 0.0024166097 1.301753e-18 7.068306e-17 2 197418952 197419640 689 - 1.952 1.376 -1.958
ENSG00000115524 E041 76.1347988 0.0007881987 2.741894e-19 1.615185e-17 2 197419641 197420180 540 - 1.842 1.188 -2.249
ENSG00000115524 E042 40.8251031 0.0079722847 3.296316e-07 3.023956e-06 2 197420181 197420427 247 - 1.569 1.066 -1.760
ENSG00000115524 E043 501.4965198 0.0036543143 6.936648e-11 1.257418e-09 2 197420428 197420542 115 - 2.620 2.404 -0.721
ENSG00000115524 E044 472.8822584 0.0005152209 2.030260e-18 1.076783e-16 2 197421029 197421133 105 - 2.592 2.399 -0.644
ENSG00000115524 E045 427.6571966 0.0030055226 4.506599e-11 8.426388e-10 2 197423808 197423893 86 - 2.551 2.337 -0.713
ENSG00000115524 E046 378.0657677 0.0001678745 2.565653e-21 1.906847e-19 2 197423894 197423974 81 - 2.499 2.275 -0.748
ENSG00000115524 E047 270.7457866 0.0001586120 3.272563e-16 1.320505e-14 2 197434972 197435079 108 - 2.355 2.129 -0.757