ENSG00000115457

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000233809 ENSG00000115457 HEK293_OSMI2_2hA HEK293_TMG_2hB IGFBP2 protein_coding protein_coding 110.1301 201.1194 54.77013 7.944285 2.797235 -1.876399 67.75492 103.61060 42.91513 11.207124 1.798378 -1.271416 0.6346458 0.5124000 0.7868000 0.2744000 0.005558638 0.005558638 FALSE TRUE
ENST00000456764 ENSG00000115457 HEK293_OSMI2_2hA HEK293_TMG_2hB IGFBP2 protein_coding protein_coding 110.1301 201.1194 54.77013 7.944285 2.797235 -1.876399 41.78172 96.57345 11.52819 4.030169 3.076091 -3.065359 0.3604500 0.4829333 0.2072667 -0.2756667 0.007095230 0.005558638 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000115457 E001 0.1515154 0.0427640383 1.121298e-01   2 216632828 216633014 187 + 0.218 0.000 -11.683
ENSG00000115457 E002 18.5764700 0.0241596253 5.017353e-08 5.410225e-07 2 216633420 216633428 9 + 1.588 0.996 -2.082
ENSG00000115457 E003 894.9501217 0.0047295984 4.932797e-06 3.514889e-05 2 216633429 216633965 537 + 2.954 2.798 -0.519
ENSG00000115457 E004 0.7331471 0.0172671820 3.935507e-01 5.374524e-01 2 216659618 216659746 129 + 0.000 0.218 12.232
ENSG00000115457 E005 1767.0013861 0.0002374841 9.703297e-01 9.854247e-01 2 216660557 216660732 176 + 3.133 3.121 -0.039
ENSG00000115457 E006 1135.4410566 0.0005503358 3.076258e-02 7.344801e-02 2 216660733 216660786 54 + 2.913 2.936 0.078
ENSG00000115457 E007 8.6948370 0.0020066322 8.589290e-03 2.526182e-02 2 216660787 216661771 985 + 1.126 0.805 -1.200
ENSG00000115457 E008 8.6539728 0.0020473793 6.022055e-01 7.232078e-01 2 216661772 216661857 86 + 0.945 0.866 -0.297
ENSG00000115457 E009 1789.4015756 0.0003357794 2.330465e-01 3.660404e-01 2 216661858 216661998 141 + 3.127 3.130 0.010
ENSG00000115457 E010 12.0694620 0.0171771443 3.862062e-01 5.303970e-01 2 216661999 216663939 1941 + 1.104 0.988 -0.422
ENSG00000115457 E011 2087.5784396 0.0006169784 2.439981e-05 1.484082e-04 2 216663940 216664436 497 + 3.160 3.203 0.144