ENSG00000115271

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000437150 ENSG00000115271 HEK293_OSMI2_2hA HEK293_TMG_2hB GCA protein_coding protein_coding 12.25313 6.923165 16.67703 1.512879 1.261233 1.267142 2.172289 1.934481 3.036048 0.3206135 0.1779303 0.6475534 0.2033667 0.2854000 0.1855000 -0.0999 1.113110e-01 2.453841e-13 FALSE TRUE
ENST00000479199 ENSG00000115271 HEK293_OSMI2_2hA HEK293_TMG_2hB GCA protein_coding retained_intron 12.25313 6.923165 16.67703 1.512879 1.261233 1.267142 7.031062 4.301923 9.486076 0.9832619 0.3739724 1.1390002 0.5627500 0.6183667 0.5724667 -0.0459 6.358621e-01 2.453841e-13 FALSE TRUE
ENST00000481161 ENSG00000115271 HEK293_OSMI2_2hA HEK293_TMG_2hB GCA protein_coding retained_intron 12.25313 6.923165 16.67703 1.512879 1.261233 1.267142 1.748683 0.000000 3.057024 0.0000000 0.6366815 8.2606955 0.1235750 0.0000000 0.1806000 0.1806 2.453841e-13 2.453841e-13 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000115271 E001 0.0000000       2 162318840 162318883 44 +      
ENSG00000115271 E002 0.0000000       2 162318884 162319033 150 +      
ENSG00000115271 E003 0.2966881 0.0271064195 3.526780e-01   2 162319034 162319183 150 + 0.186 0.000 -11.068
ENSG00000115271 E004 0.1515154 0.0433439866 7.460997e-01   2 162319184 162319225 42 + 0.103 0.000 -10.068
ENSG00000115271 E005 0.1515154 0.0433439866 7.460997e-01   2 162319226 162319239 14 + 0.103 0.000 -10.068
ENSG00000115271 E006 0.3666179 0.0305530818 7.601856e-01 8.443600e-01 2 162319282 162319395 114 + 0.103 0.145 0.577
ENSG00000115271 E007 1.7661916 0.0143497305 5.840255e-02 1.238921e-01 2 162344132 162344133 2 + 0.255 0.578 1.799
ENSG00000115271 E008 2.0586133 0.0099995425 2.040159e-01 3.312013e-01 2 162344134 162344134 1 + 0.368 0.578 1.061
ENSG00000115271 E009 10.7602691 0.0141695024 4.407702e-01 5.824313e-01 2 162344135 162344158 24 + 1.105 1.007 -0.357
ENSG00000115271 E010 26.0615520 0.0057414764 8.240812e-02 1.633051e-01 2 162344159 162344181 23 + 1.486 1.344 -0.492
ENSG00000115271 E011 54.5791309 0.0076824733 4.304096e-02 9.662032e-02 2 162344182 162344275 94 + 1.799 1.661 -0.466
ENSG00000115271 E012 3.8602949 0.0487741504 4.360263e-01 5.781589e-01 2 162344338 162344525 188 + 0.623 0.733 0.463
ENSG00000115271 E013 1.6532762 0.0212987565 1.332274e-01 2.386197e-01 2 162346582 162346665 84 + 0.531 0.254 -1.597
ENSG00000115271 E014 108.5967074 0.0004272407 7.853828e-03 2.341465e-02 2 162347578 162347742 165 + 2.081 1.990 -0.304
ENSG00000115271 E015 75.0199237 0.0004421560 7.451463e-01 8.332885e-01 2 162352338 162352407 70 + 1.882 1.892 0.033
ENSG00000115271 E016 68.6403794 0.0003580646 6.048710e-01 7.252962e-01 2 162356438 162356481 44 + 1.855 1.830 -0.084
ENSG00000115271 E017 1.1845624 0.0107161229 8.954164e-03 2.616475e-02 2 162356482 162356665 184 + 0.496 0.000 -13.067
ENSG00000115271 E018 103.6781000 0.0003056812 2.369356e-01 3.706278e-01 2 162356758 162356905 148 + 2.042 1.999 -0.143
ENSG00000115271 E019 0.6717251 0.0191704337 5.464328e-01 6.766131e-01 2 162356906 162356981 76 + 0.255 0.145 -1.010
ENSG00000115271 E020 79.9379827 0.0003381661 7.815928e-01 8.596849e-01 2 162359044 162359157 114 + 1.909 1.916 0.023
ENSG00000115271 E021 6.7610926 0.0274347158 8.479267e-01 9.055746e-01 2 162359158 162359493 336 + 0.885 0.908 0.089
ENSG00000115271 E022 62.6893083 0.0004756456 3.006984e-01 4.424335e-01 2 162359494 162359552 59 + 1.791 1.833 0.140
ENSG00000115271 E023 131.6628306 0.0004217328 8.177775e-07 6.920024e-06 2 162360217 162360745 529 + 2.058 2.197 0.466
ENSG00000115271 E024 68.1612797 0.0004693260 5.577623e-01 6.861142e-01 2 162360746 162363123 2378 + 1.834 1.855 0.071
ENSG00000115271 E025 1.2187125 0.0149718690 6.592940e-03 2.016749e-02 2 162371306 162371595 290 + 0.103 0.576 3.374