ENSG00000114853

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000505904 ENSG00000114853 HEK293_OSMI2_2hA HEK293_TMG_2hB ZBTB47 protein_coding protein_coding 8.531726 13.51159 5.502884 1.035142 0.1319076 -1.294386 0.6755820 2.238290 0.0000000 1.137438 0.0000000 -7.8126843 0.06153333 0.17086667 0.00000000 -0.17086667 0.24094740 0.02795457 FALSE TRUE
ENST00000680014 ENSG00000114853 HEK293_OSMI2_2hA HEK293_TMG_2hB ZBTB47 protein_coding protein_coding 8.531726 13.51159 5.502884 1.035142 0.1319076 -1.294386 4.8195570 5.254834 4.4866258 0.233257 0.5001581 -0.2275452 0.55825833 0.39266667 0.81823333 0.42556667 0.10529395 0.02795457 FALSE TRUE
MSTRG.22816.5 ENSG00000114853 HEK293_OSMI2_2hA HEK293_TMG_2hB ZBTB47 protein_coding   8.531726 13.51159 5.502884 1.035142 0.1319076 -1.294386 2.0863187 5.705989 0.2080223 1.309219 0.2080223 -4.7124556 0.25491667 0.41330000 0.03933333 -0.37396667 0.02795457 0.02795457 FALSE TRUE
MSTRG.22816.6 ENSG00000114853 HEK293_OSMI2_2hA HEK293_TMG_2hB ZBTB47 protein_coding   8.531726 13.51159 5.502884 1.035142 0.1319076 -1.294386 0.8110336 0.312478 0.6825847 0.312478 0.6825847 1.1027898 0.10579583 0.02316667 0.11883333 0.09566667 0.94453421 0.02795457 TRUE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000114853 E001 0.0000000       3 42653697 42653883 187 +      
ENSG00000114853 E002 0.7342825 0.0172734640 7.204430e-04 2.972186e-03 3 42654504 42654504 1 + 0.550 0.000 -13.974
ENSG00000114853 E003 4.4965271 0.0034505467 1.306992e-12 3.144999e-11 3 42654505 42654575 71 + 1.170 0.210 -4.468
ENSG00000114853 E004 14.9385798 0.0189225156 2.729089e-05 1.639608e-04 3 42654576 42654695 120 + 1.415 0.997 -1.484
ENSG00000114853 E005 0.6568746 0.0209431871 9.201728e-01 9.535801e-01 3 42655138 42655190 53 + 0.179 0.210 0.286
ENSG00000114853 E006 142.9838019 0.0066968347 3.196784e-04 1.459609e-03 3 42658275 42658813 539 + 2.193 2.053 -0.469
ENSG00000114853 E007 47.4061427 0.0066922967 8.215336e-01 8.874480e-01 3 42658814 42658847 34 + 1.611 1.623 0.042
ENSG00000114853 E008 99.1900387 0.0004056266 8.770415e-02 1.716473e-01 3 42658848 42659035 188 + 1.963 1.922 -0.138
ENSG00000114853 E009 94.7610302 0.0003750641 1.748387e-04 8.561926e-04 3 42659036 42659238 203 + 2.002 1.882 -0.404
ENSG00000114853 E010 21.6283764 0.0009053092 1.028194e-01 1.947901e-01 3 42659239 42659340 102 + 1.370 1.266 -0.365
ENSG00000114853 E011 87.4263919 0.0052680727 1.295620e-01 2.335385e-01 3 42659341 42659636 296 + 1.797 1.909 0.375
ENSG00000114853 E012 113.6101071 0.0015983624 9.028259e-02 1.755691e-01 3 42659637 42659828 192 + 1.920 2.017 0.327
ENSG00000114853 E013 104.6305303 0.0003116937 5.359240e-01 6.677189e-01 3 42661485 42661632 148 + 1.923 1.972 0.167
ENSG00000114853 E014 113.7277144 0.0003079624 9.822452e-01 9.929426e-01 3 42663012 42663127 116 + 1.977 2.002 0.083
ENSG00000114853 E015 120.1440906 0.0003163092 8.171136e-01 8.843425e-01 3 42663797 42663941 145 + 2.007 2.024 0.058
ENSG00000114853 E016 959.6871201 0.0060441917 9.105826e-03 2.654691e-02 3 42664237 42667580 3344 + 2.851 2.941 0.300