ENSG00000114784

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000232905 ENSG00000114784 HEK293_OSMI2_2hA HEK293_TMG_2hB EIF1B protein_coding protein_coding 126.8736 138.1708 159.2464 9.348415 7.046148 0.2047948 91.44401 128.998432 90.80907 10.845185 3.449745 -0.5063983 0.730075 0.9332 0.5708000 -0.3624000 0.02255508 0.0008087251 FALSE TRUE
MSTRG.22772.1 ENSG00000114784 HEK293_OSMI2_2hA HEK293_TMG_2hB EIF1B protein_coding   126.8736 138.1708 159.2464 9.348415 7.046148 0.2047948 33.04554 8.334125 64.97241 5.539332 4.847952 2.9612166 0.251200 0.0609 0.4071667 0.3462667 0.21129312 0.0008087251 TRUE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000114784 E001 28.6587529 6.726308e-04 5.432994e-01 6.739287e-01 3 40309707 40309710 4 + 1.446 1.493 0.164
ENSG00000114784 E002 124.3967352 2.557296e-04 1.567075e-11 3.160095e-10 3 40309711 40309721 11 + 2.201 1.995 -0.691
ENSG00000114784 E003 621.9721082 4.755790e-03 1.464693e-01 2.568824e-01 3 40309722 40309825 104 + 2.819 2.768 -0.170
ENSG00000114784 E004 1217.0873284 1.661707e-03 1.614704e-02 4.305310e-02 3 40309826 40309972 147 + 3.106 3.063 -0.144
ENSG00000114784 E005 6.4600149 2.690981e-03 4.930254e-03 1.573566e-02 3 40310628 40310892 265 + 1.037 0.702 -1.290
ENSG00000114784 E006 1307.1901800 6.955983e-05 4.492967e-04 1.964048e-03 3 40310893 40311056 164 + 3.130 3.105 -0.082
ENSG00000114784 E007 11.8205806 1.498541e-03 2.194954e-07 2.087801e-06 3 40311057 40311469 413 + 1.324 0.819 -1.845
ENSG00000114784 E008 1066.9926072 8.425282e-04 8.553954e-01 9.105892e-01 3 40311470 40311571 102 + 3.021 3.031 0.031
ENSG00000114784 E009 11.5481283 7.674336e-03 3.211183e-04 1.465407e-03 3 40311572 40311795 224 + 1.272 0.910 -1.315
ENSG00000114784 E010 1473.0857701 1.671511e-03 5.361988e-02 1.155869e-01 3 40311970 40312169 200 + 3.143 3.182 0.131
ENSG00000114784 E011 687.9261527 1.148922e-03 6.142986e-19 3.472904e-17 3 40312170 40312424 255 + 2.722 2.908 0.617
ENSG00000114784 E012 0.2966881 2.907852e-02 1.762813e-01   3 40316602 40316644 43 + 0.226 0.000 -10.838
ENSG00000114784 E013 0.3030308 4.019951e-01 2.689751e-01   3 40324355 40324388 34 + 0.227 0.000 -10.846