ENSG00000114742

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000302313 ENSG00000114742 HEK293_OSMI2_2hA HEK293_TMG_2hB WDR48 protein_coding protein_coding 25.68467 9.052223 38.72231 0.5366241 1.644413 2.095601 18.1863528 8.1808842 24.300639 0.3184257 1.3451984 1.569497 0.77568750 0.90783333 0.62990000 -0.27793333 1.245083e-01 1.157052e-09 FALSE TRUE
ENST00000413099 ENSG00000114742 HEK293_OSMI2_2hA HEK293_TMG_2hB WDR48 protein_coding nonsense_mediated_decay 25.68467 9.052223 38.72231 0.5366241 1.644413 2.095601 2.3780408 0.0000000 3.888165 0.0000000 0.8832425 8.606651 0.05582083 0.00000000 0.09936667 0.09936667 1.157052e-09 1.157052e-09 FALSE TRUE
ENST00000423296 ENSG00000114742 HEK293_OSMI2_2hA HEK293_TMG_2hB WDR48 protein_coding nonsense_mediated_decay 25.68467 9.052223 38.72231 0.5366241 1.644413 2.095601 0.5134228 0.0000000 2.107819 0.0000000 1.5275092 7.726436 0.01216667 0.00000000 0.05146667 0.05146667 3.873253e-01 1.157052e-09 TRUE TRUE
MSTRG.22757.1 ENSG00000114742 HEK293_OSMI2_2hA HEK293_TMG_2hB WDR48 protein_coding   25.68467 9.052223 38.72231 0.5366241 1.644413 2.095601 2.3029227 0.6693218 4.357344 0.3641119 0.4420566 2.684589 0.06894167 0.07116667 0.11310000 0.04193333 6.703979e-01 1.157052e-09 TRUE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000114742 E001 3.1000713 0.0048494775 2.972083e-02 7.140892e-02 3 39051956 39052012 57 + 0.623 0.221 -2.269
ENSG00000114742 E002 8.0363829 0.0021590972 4.601618e-03 1.482447e-02 3 39052013 39052015 3 + 0.954 0.562 -1.591
ENSG00000114742 E003 8.9857679 0.0049540413 6.627672e-03 2.025742e-02 3 39052016 39052018 3 + 0.990 0.635 -1.406
ENSG00000114742 E004 10.1899101 0.0190482134 1.123276e-02 3.172351e-02 3 39052019 39052019 1 + 1.037 0.697 -1.314
ENSG00000114742 E005 71.4799682 0.0032979449 1.724726e-03 6.360604e-03 3 39052020 39052073 54 + 1.810 1.682 -0.433
ENSG00000114742 E006 0.8889184 0.0134853052 7.692776e-01 8.509563e-01 3 39052301 39052601 301 + 0.255 0.221 -0.270
ENSG00000114742 E007 142.3707407 0.0019571008 5.253261e-08 5.637970e-07 3 39063050 39063190 141 + 2.115 1.937 -0.596
ENSG00000114742 E008 0.2924217 0.0272956981 1.000000e+00   3 39065791 39065810 20 + 0.120 0.000 -9.587
ENSG00000114742 E009 129.7174383 0.0011143106 3.322749e-07 3.045825e-06 3 39065811 39065889 79 + 2.070 1.913 -0.528
ENSG00000114742 E010 139.5829822 0.0161158809 2.748472e-03 9.517037e-03 3 39066548 39066630 83 + 2.101 1.948 -0.515
ENSG00000114742 E011 0.2924217 0.0272956981 1.000000e+00   3 39066727 39066745 19 + 0.120 0.000 -9.587
ENSG00000114742 E012 165.7884949 0.0018358820 7.788742e-09 9.795232e-08 3 39066746 39066875 130 + 2.178 2.002 -0.590
ENSG00000114742 E013 150.0178450 0.0002649837 1.086879e-10 1.907243e-09 3 39068771 39068859 89 + 2.138 1.957 -0.608
ENSG00000114742 E014 165.1646264 0.0002491549 8.979166e-08 9.192522e-07 3 39069643 39069744 102 + 2.169 2.043 -0.421
ENSG00000114742 E015 178.7361855 0.0001933530 4.452388e-04 1.948423e-03 3 39074726 39074950 225 + 2.189 2.136 -0.176
ENSG00000114742 E016 95.4884122 0.0018702829 7.847344e-02 1.570339e-01 3 39077139 39077213 75 + 1.912 1.888 -0.082
ENSG00000114742 E017 1.0276347 0.1559494404 6.766375e-01 7.815610e-01 3 39078011 39078136 126 + 0.291 0.219 -0.541
ENSG00000114742 E018 72.6673365 0.0003375578 3.413497e-01 4.852520e-01 3 39078137 39078160 24 + 1.788 1.802 0.046
ENSG00000114742 E019 128.0015012 0.0002336929 2.154999e-03 7.704874e-03 3 39078161 39078239 79 + 2.047 1.990 -0.190
ENSG00000114742 E020 152.6756737 0.0017683955 1.139649e-02 3.210980e-02 3 39079711 39079808 98 + 2.118 2.076 -0.139
ENSG00000114742 E021 148.6391052 0.0031960555 2.852645e-02 6.906743e-02 3 39084155 39084262 108 + 2.105 2.067 -0.130
ENSG00000114742 E022 110.2510785 0.0033557090 5.703021e-01 6.968025e-01 3 39084645 39084741 97 + 1.948 2.033 0.284
ENSG00000114742 E023 134.1222512 0.0009513058 7.075666e-01 8.054584e-01 3 39085515 39085610 96 + 2.035 2.108 0.247
ENSG00000114742 E024 2.0755572 0.0068770552 1.404260e-01 2.486149e-01 3 39086301 39086384 84 + 0.488 0.221 -1.648
ENSG00000114742 E025 5.0040633 0.0048218197 6.211836e-01 7.381891e-01 3 39087818 39088127 310 + 0.725 0.697 -0.117
ENSG00000114742 E026 146.7896244 0.0006210236 2.625652e-01 4.003545e-01 3 39088128 39088233 106 + 2.087 2.106 0.066
ENSG00000114742 E027 140.5617464 0.0014704761 2.769239e-02 6.740380e-02 3 39089231 39089318 88 + 2.080 2.051 -0.096
ENSG00000114742 E028 18.4597823 0.0010050773 3.048068e-03 1.040783e-02 3 39089568 39091624 2057 + 1.265 1.012 -0.908
ENSG00000114742 E029 129.8367001 0.0026628870 1.222994e-01 2.232257e-01 3 39091625 39091701 77 + 2.041 2.031 -0.035
ENSG00000114742 E030 187.1528796 0.0008818139 1.526417e-01 2.652642e-01 3 39093874 39094066 193 + 2.195 2.208 0.042
ENSG00000114742 E031 1.3381542 0.0095197229 7.213471e-02 1.468878e-01 3 39094067 39094647 581 + 0.387 0.000 -11.685
ENSG00000114742 E032 453.2312450 0.0003737879 1.010037e-04 5.265721e-04 3 39094648 39095414 767 + 2.545 2.683 0.456
ENSG00000114742 E033 80.3957817 0.0013145132 8.249552e-09 1.032881e-07 3 39095415 39095480 66 + 1.738 2.056 1.071
ENSG00000114742 E034 593.8489747 0.0078820861 1.278913e-18 6.949705e-17 3 39095481 39096671 1191 + 2.568 2.973 1.349