ENSG00000114648

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000232766 ENSG00000114648 HEK293_OSMI2_2hA HEK293_TMG_2hB KLHL18 protein_coding protein_coding 27.74673 37.33431 22.01976 2.13079 1.067517 -0.7614349 9.925542 8.960104 8.702758 0.5649580 0.4139288 -0.04199521 0.3772792 0.2404667 0.3982667 0.1578000 4.057204e-03 3.089893e-15 FALSE TRUE
ENST00000483201 ENSG00000114648 HEK293_OSMI2_2hA HEK293_TMG_2hB KLHL18 protein_coding processed_transcript 27.74673 37.33431 22.01976 2.13079 1.067517 -0.7614349 10.652656 20.048139 4.772790 1.7720139 0.5468821 -2.06826352 0.3530750 0.5353333 0.2155333 -0.3198000 3.089893e-15 3.089893e-15   FALSE
MSTRG.22877.1 ENSG00000114648 HEK293_OSMI2_2hA HEK293_TMG_2hB KLHL18 protein_coding   27.74673 37.33431 22.01976 2.13079 1.067517 -0.7614349 5.276119 5.147789 5.635273 0.2058268 0.7322583 0.13029078 0.2004333 0.1390000 0.2576333 0.1186333 4.055741e-02 3.089893e-15 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000114648 E001 15.563351 0.0023892596 4.449850e-01 5.865584e-01 3 47282822 47282916 95 + 1.227 1.214 -0.045
ENSG00000114648 E002 18.158441 0.0011751594 5.141571e-01 6.489073e-01 3 47282917 47282943 27 + 1.218 1.324 0.372
ENSG00000114648 E003 17.646739 0.0010967076 6.862000e-01 7.887637e-01 3 47282944 47282945 2 + 1.218 1.304 0.303
ENSG00000114648 E004 17.465034 0.0010718694 7.495041e-01 8.364484e-01 3 47282946 47282946 1 + 1.218 1.297 0.279
ENSG00000114648 E005 30.486180 0.0006690440 5.809590e-01 7.056048e-01 3 47282947 47282958 12 + 1.482 1.502 0.068
ENSG00000114648 E006 139.399896 0.0045050907 8.480278e-03 2.498660e-02 3 47282959 47283094 136 + 2.040 2.208 0.565
ENSG00000114648 E007 67.701240 0.0015963390 1.987567e-22 1.661033e-20 3 47287220 47287348 129 + 1.431 1.997 1.921
ENSG00000114648 E008 26.795089 0.0007300571 5.260475e-16 2.060755e-14 3 47287349 47287458 110 + 0.925 1.631 2.492
ENSG00000114648 E009 1.347249 0.5912337470 9.312240e-01 9.606833e-01 3 47297562 47297672 111 + 0.369 0.362 -0.042
ENSG00000114648 E010 95.697973 0.0029996534 1.851281e-06 1.450933e-05 3 47319653 47319783 131 + 2.062 1.908 -0.518
ENSG00000114648 E011 96.183403 0.0084929856 3.538432e-04 1.594959e-03 3 47322568 47322708 141 + 2.060 1.915 -0.487
ENSG00000114648 E012 117.344593 0.0029344624 3.687818e-04 1.653365e-03 3 47329951 47330149 199 + 2.118 2.029 -0.299
ENSG00000114648 E013 102.618267 0.0003850022 1.611745e-03 5.998591e-03 3 47333157 47333317 161 + 2.043 1.988 -0.182
ENSG00000114648 E014 92.588060 0.0003143901 8.743885e-05 4.630077e-04 3 47334683 47334819 137 + 2.017 1.930 -0.291
ENSG00000114648 E015 17.580807 0.0010360399 3.760938e-02 8.659541e-02 3 47336520 47336534 15 + 1.327 1.214 -0.396
ENSG00000114648 E016 161.416962 0.0002395482 4.141350e-08 4.542418e-07 3 47336535 47336757 223 + 2.257 2.162 -0.319
ENSG00000114648 E017 17.748422 0.0038179173 1.204162e-03 4.654660e-03 3 47336758 47338822 2065 + 1.379 1.161 -0.766
ENSG00000114648 E018 128.772036 0.0012854180 1.162126e-05 7.608031e-05 3 47340572 47340676 105 + 2.160 2.063 -0.322
ENSG00000114648 E019 108.266447 0.0002710407 5.710958e-07 4.997339e-06 3 47342719 47342830 112 + 2.094 1.981 -0.377
ENSG00000114648 E020 1584.349724 0.0041184307 9.838702e-05 5.143530e-04 3 47343555 47346816 3262 + 3.127 3.244 0.387