ENSG00000114520

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000251775 ENSG00000114520 HEK293_OSMI2_2hA HEK293_TMG_2hB SNX4 protein_coding protein_coding 17.58103 4.831431 33.14324 0.3433193 0.7578254 2.775644 13.234554 4.5357250 24.590758 0.3743766 1.3088076 2.436120 0.82554167 0.93753333 0.7412333 -0.1963000 6.863312e-05 6.863312e-05 FALSE TRUE
ENST00000471751 ENSG00000114520 HEK293_OSMI2_2hA HEK293_TMG_2hB SNX4 protein_coding nonsense_mediated_decay 17.58103 4.831431 33.14324 0.3433193 0.7578254 2.775644 2.695628 0.1421404 5.679112 0.1213832 0.1296462 5.224728 0.09805417 0.03153333 0.1716667 0.1401333 4.525563e-02 6.863312e-05 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000114520 E001 178.1409700 0.0285605527 8.480558e-06 5.727687e-05 3 125446650 125447150 501 - 2.012 2.446 1.450
ENSG00000114520 E002 190.8270821 0.0073524827 3.275633e-03 1.107923e-02 3 125447151 125447467 317 - 2.106 2.309 0.678
ENSG00000114520 E003 112.3735544 0.0002665529 2.191056e-02 5.549244e-02 3 125447468 125447710 243 - 1.887 2.037 0.502
ENSG00000114520 E004 106.7851518 0.0021034653 7.613270e-01 8.452264e-01 3 125447711 125447826 116 - 1.879 1.944 0.219
ENSG00000114520 E005 0.5922303 0.0179979554 5.601995e-01 6.882185e-01 3 125449070 125449255 186 - 0.139 0.265 1.158
ENSG00000114520 E006 4.0972034 0.0995370804 7.169034e-01 8.124377e-01 3 125449297 125449411 115 - 0.605 0.546 -0.266
ENSG00000114520 E007 158.0699706 0.0010572427 2.200490e-01 3.504693e-01 3 125451305 125451419 115 - 2.058 2.060 0.006
ENSG00000114520 E008 179.5729687 0.0014091734 8.740567e-01 9.230270e-01 3 125453810 125453955 146 - 2.107 2.152 0.152
ENSG00000114520 E009 147.4871854 0.0002398471 4.686603e-01 6.080711e-01 3 125457266 125457365 100 - 2.026 2.050 0.080
ENSG00000114520 E010 98.8895769 0.0002789747 1.349951e-01 2.410816e-01 3 125460771 125460860 90 - 1.862 1.840 -0.073
ENSG00000114520 E011 0.2966881 0.0270045461 1.000000e+00   3 125469444 125469453 10 - 0.097 0.001 -7.269
ENSG00000114520 E012 95.3891558 0.0002906551 2.326566e-01 3.655977e-01 3 125469454 125469519 66 - 1.843 1.835 -0.026
ENSG00000114520 E013 6.3933840 0.0034726025 6.905547e-01 7.922312e-01 3 125470566 125470661 96 - 0.732 0.839 0.423
ENSG00000114520 E014 94.7155248 0.0002902130 6.153578e-03 1.901587e-02 3 125476695 125476756 62 - 1.851 1.759 -0.310
ENSG00000114520 E015 116.1605794 0.0002722500 3.970110e-06 2.890566e-05 3 125480247 125480319 73 - 1.949 1.772 -0.598
ENSG00000114520 E016 105.2464226 0.0003047176 1.840094e-03 6.726750e-03 3 125489408 125489463 56 - 1.900 1.796 -0.351
ENSG00000114520 E017 100.0829354 0.0003978165 3.539787e-02 8.242000e-02 3 125497341 125497388 48 - 1.872 1.819 -0.181
ENSG00000114520 E018 157.2838736 0.0006490782 7.097789e-05 3.849129e-04 3 125497834 125497983 150 - 2.072 1.956 -0.391
ENSG00000114520 E019 150.9162021 0.0002607479 7.596485e-04 3.113587e-03 3 125498059 125498194 136 - 2.051 1.964 -0.293
ENSG00000114520 E020 126.0318342 0.0002376683 1.349170e-02 3.706011e-02 3 125504623 125504744 122 - 1.971 1.913 -0.194
ENSG00000114520 E021 84.2005778 0.0003232336 7.215096e-01 8.158532e-01 3 125520032 125520279 248 - 1.785 1.819 0.114