ENSG00000114446

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000465024 ENSG00000114446 HEK293_OSMI2_2hA HEK293_TMG_2hB IFT57 protein_coding retained_intron 55.25109 23.46725 89.48764 1.195216 1.143822 1.930586 5.881059 0.6888422 17.10940 0.3946858 0.2025351 4.614522 0.07703333 0.03096667 0.1912333 0.16026667 1.161679e-01 5.451482e-05 FALSE TRUE
ENST00000478157 ENSG00000114446 HEK293_OSMI2_2hA HEK293_TMG_2hB IFT57 protein_coding nonsense_mediated_decay 55.25109 23.46725 89.48764 1.195216 1.143822 1.930586 8.342287 1.6595566 13.41675 0.4895544 1.3141220 3.007573 0.13881667 0.06913333 0.1496000 0.08046667 5.155774e-02 5.451482e-05 FALSE FALSE
MSTRG.23405.2 ENSG00000114446 HEK293_OSMI2_2hA HEK293_TMG_2hB IFT57 protein_coding   55.25109 23.46725 89.48764 1.195216 1.143822 1.930586 15.921497 9.4873441 23.90259 0.5418844 0.8296177 1.332174 0.31568333 0.40460000 0.2674333 -0.13716667 5.451482e-05 5.451482e-05 FALSE TRUE
MSTRG.23405.4 ENSG00000114446 HEK293_OSMI2_2hA HEK293_TMG_2hB IFT57 protein_coding   55.25109 23.46725 89.48764 1.195216 1.143822 1.930586 21.515372 10.2647445 29.85285 0.8755160 0.6027475 1.539250 0.40633750 0.43740000 0.3335667 -0.10383333 4.063110e-02 5.451482e-05 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000114446 E001 39.4502809 0.0088698409 7.658014e-02 1.540213e-01 3 108160812 108162259 1448 - 1.616 1.486 -0.442
ENSG00000114446 E002 11.3146935 0.0277298417 3.507761e-03 1.174878e-02 3 108162260 108162318 59 - 0.921 1.279 1.295
ENSG00000114446 E003 18.5334980 0.0196172993 4.446962e-05 2.536700e-04 3 108162319 108162339 21 - 1.090 1.512 1.481
ENSG00000114446 E004 327.3646323 0.0030901186 5.905827e-04 2.499199e-03 3 108162340 108162655 316 - 2.447 2.559 0.374
ENSG00000114446 E005 282.7993486 0.0008153863 7.441601e-02 1.506008e-01 3 108163663 108163729 67 - 2.405 2.451 0.154
ENSG00000114446 E006 180.6867925 0.0002357837 1.953206e-01 3.203700e-01 3 108165431 108165434 4 - 2.215 2.252 0.126
ENSG00000114446 E007 284.7908401 0.0002156346 6.498058e-02 1.350029e-01 3 108165435 108165493 59 - 2.410 2.452 0.142
ENSG00000114446 E008 372.5146152 0.0001580624 4.849174e-02 1.064954e-01 3 108166854 108166985 132 - 2.527 2.567 0.131
ENSG00000114446 E009 3.6622250 0.0042956946 2.204946e-02 5.578382e-02 3 108166986 108167176 191 - 0.729 0.316 -2.029
ENSG00000114446 E010 5.0002654 0.0072125799 2.324289e-03 8.226154e-03 3 108167622 108167792 171 - 0.854 0.316 -2.524
ENSG00000114446 E011 295.2562198 0.0002230983 5.831782e-02 1.237415e-01 3 108167793 108167864 72 - 2.426 2.469 0.144
ENSG00000114446 E012 340.5311809 0.0001443488 2.272162e-01 3.590830e-01 3 108191521 108191643 123 - 2.492 2.517 0.084
ENSG00000114446 E013 243.0643796 0.0006337079 2.050766e-01 3.325015e-01 3 108206628 108206692 65 - 2.343 2.376 0.110
ENSG00000114446 E014 135.9901578 0.0024527213 5.593330e-01 6.874571e-01 3 108206693 108206696 4 - 2.096 2.117 0.072
ENSG00000114446 E015 3.9149284 0.0234165636 9.108940e-01 9.473847e-01 3 108213799 108213930 132 - 0.660 0.676 0.067
ENSG00000114446 E016 275.5368686 0.0011068703 4.884943e-01 6.260487e-01 3 108213931 108214021 91 - 2.414 2.394 -0.066
ENSG00000114446 E017 6.6354361 0.0479124435 4.013097e-01 5.450036e-01 3 108215278 108215388 111 - 0.803 0.932 0.497
ENSG00000114446 E018 0.5106578 0.3630841062 2.364093e-01 3.700062e-01 3 108215389 108215392 4 - 0.079 0.317 2.440
ENSG00000114446 E019 2.1163527 0.0201823256 3.901894e-01 5.342000e-01 3 108218393 108218534 142 - 0.413 0.565 0.752
ENSG00000114446 E020 287.1503575 0.0017934246 2.810045e-03 9.704405e-03 3 108218535 108218653 119 - 2.452 2.360 -0.307
ENSG00000114446 E021 0.9630129 0.0152948292 4.752604e-02 1.047766e-01 3 108219405 108219409 5 - 0.145 0.496 2.427
ENSG00000114446 E022 318.8451428 0.0007476825 1.642643e-05 1.038975e-04 3 108219410 108219572 163 - 2.501 2.393 -0.362
ENSG00000114446 E023 1.0382412 0.0129706890 4.642832e-02 1.028058e-01 3 108222018 108222110 93 - 0.145 0.497 2.430
ENSG00000114446 E024 415.7902367 0.0007621828 2.627102e-13 7.039555e-12 3 108222111 108222435 325 - 2.631 2.463 -0.559