ENSG00000111266

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000298573 ENSG00000111266 HEK293_OSMI2_2hA HEK293_TMG_2hB DUSP16 protein_coding protein_coding 5.083508 4.033808 4.092629 0.2773553 0.1239344 0.0208342 4.1431072 3.8466016 3.3100429 0.29249764 0.1875594 -0.2161283 0.83292500 0.9534 0.8088667 -0.1445333 0.085811939 0.005230498 FALSE TRUE
MSTRG.6843.2 ENSG00000111266 HEK293_OSMI2_2hA HEK293_TMG_2hB DUSP16 protein_coding   5.083508 4.033808 4.092629 0.2773553 0.1239344 0.0208342 0.4646175 0.1176398 0.7825866 0.04731641 0.1644967 2.6344905 0.08890417 0.0302 0.1911333 0.1609333 0.005230498 0.005230498 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000111266 E001 276.0152207 0.0027812283 3.848897e-07 3.480986e-06 12 12473282 12475896 2615 - 2.349 2.499 0.501
ENSG00000111266 E002 274.2694786 0.0004087351 1.493464e-01 2.607719e-01 12 12475897 12477885 1989 - 2.412 2.457 0.153
ENSG00000111266 E003 32.9359005 0.0006718858 7.204056e-01 8.150219e-01 12 12477886 12478015 130 - 1.503 1.543 0.137
ENSG00000111266 E004 34.0224403 0.0237071459 8.551486e-01 9.104236e-01 12 12480223 12480346 124 - 1.539 1.535 -0.015
ENSG00000111266 E005 41.0863680 0.0032081388 6.887033e-02 1.414968e-01 12 12487028 12487187 160 - 1.669 1.580 -0.302
ENSG00000111266 E006 38.6120440 0.0017446705 2.259614e-01 3.575468e-01 12 12500519 12500682 164 - 1.629 1.579 -0.168
ENSG00000111266 E007 0.1482932 0.0407967527 5.521504e-01   12 12506088 12506187 100 - 0.000 0.117 9.190
ENSG00000111266 E008 37.1746648 0.0010151779 2.862471e-01 4.266445e-01 12 12519862 12520000 139 - 1.607 1.566 -0.141
ENSG00000111266 E009 40.8722461 0.0005751758 5.529216e-04 2.357573e-03 12 12520871 12521023 153 - 1.708 1.543 -0.562
ENSG00000111266 E010 30.6163130 0.0087623179 1.984879e-01 3.243086e-01 12 12521024 12521080 57 - 1.544 1.462 -0.279
ENSG00000111266 E011 56.6867790 0.0004954047 5.715503e-04 2.427497e-03 12 12521081 12521326 246 - 1.832 1.694 -0.465
ENSG00000111266 E012 32.2196404 0.0007294790 1.153448e-02 3.242963e-02 12 12521327 12521463 137 - 1.588 1.454 -0.458
ENSG00000111266 E013 3.4866584 0.0849780114 5.849816e-02 1.240644e-01 12 12554536 12554659 124 - 0.813 0.456 -1.565
ENSG00000111266 E014 0.0000000       12 12560766 12560999 234 -      
ENSG00000111266 E015 0.0000000       12 12561000 12561125 126 -      
ENSG00000111266 E016 22.1393673 0.0022663992 1.232589e-05 8.018897e-05 12 12562117 12562863 747 - 1.513 1.207 -1.063