ENSG00000111252

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000341259 ENSG00000111252 HEK293_OSMI2_2hA HEK293_TMG_2hB SH2B3 protein_coding protein_coding 4.055958 5.081081 3.365198 0.2328896 0.1724847 -0.5929999 1.134735 0.7083983 1.3447079 0.2112925 0.05793491 0.915126 0.2882708 0.1402333 0.4001000 0.2598667 0.001604162 0.001604162 FALSE TRUE
ENST00000538307 ENSG00000111252 HEK293_OSMI2_2hA HEK293_TMG_2hB SH2B3 protein_coding protein_coding 4.055958 5.081081 3.365198 0.2328896 0.1724847 -0.5929999 1.847066 3.6886440 0.7423742 0.4594479 0.49922033 -2.297474 0.4466125 0.7255333 0.2233333 -0.5022000 0.189796137 0.001604162 FALSE TRUE
MSTRG.8024.3 ENSG00000111252 HEK293_OSMI2_2hA HEK293_TMG_2hB SH2B3 protein_coding   4.055958 5.081081 3.365198 0.2328896 0.1724847 -0.5929999 1.031495 0.6298759 1.2781162 0.2257940 0.50996388 1.009399 0.2543708 0.1242667 0.3765667 0.2523000 0.251111220 0.001604162 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000111252 E001 7.1087888 0.0087007995 3.309399e-07 3.034783e-06 12 111405923 111406277 355 + 1.151 0.591 -2.181
ENSG00000111252 E002 8.4029437 0.0126654784 4.244407e-05 2.431847e-04 12 111418119 111418339 221 + 1.162 0.752 -1.541
ENSG00000111252 E003 19.0456912 0.0010047630 5.210738e-03 1.650464e-02 12 111418340 111418877 538 + 1.367 1.236 -0.457
ENSG00000111252 E004 0.2998086 0.0288831380 7.508785e-01   12 111420438 111420488 51 + 0.135 0.110 -0.337
ENSG00000111252 E005 0.0000000       12 111434862 111435027 166 +      
ENSG00000111252 E006 0.8836184 0.0179227720 9.772826e-01 9.898486e-01 12 111444739 111444848 110 + 0.238 0.272 0.253
ENSG00000111252 E007 0.3729606 0.0294067346 7.542668e-01 8.399675e-01 12 111446660 111446752 93 + 0.135 0.111 -0.331
ENSG00000111252 E008 17.1651607 0.0010583997 7.366346e-05 3.977641e-04 12 111446753 111446854 102 + 1.374 1.138 -0.829
ENSG00000111252 E009 18.7895820 0.0009861928 3.243453e-01 4.676986e-01 12 111446942 111447033 92 + 1.285 1.291 0.022
ENSG00000111252 E010 2.1003443 0.0091036478 2.977779e-01 4.391356e-01 12 111447122 111447124 3 + 0.551 0.438 -0.557
ENSG00000111252 E011 18.9881866 0.0015902311 1.777424e-01 2.982900e-01 12 111447125 111447219 95 + 1.301 1.278 -0.080
ENSG00000111252 E012 32.0930615 0.0030007853 7.595832e-01 8.439037e-01 12 111447330 111447544 215 + 1.464 1.529 0.223
ENSG00000111252 E013 26.7412729 0.0146336344 2.484192e-01 3.840583e-01 12 111447656 111447827 172 + 1.442 1.428 -0.047
ENSG00000111252 E014 495.6465210 0.0093323702 3.323995e-05 1.954866e-04 12 111447983 111451807 3825 + 2.590 2.739 0.496