ENSG00000111145

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000228741 ENSG00000111145 HEK293_OSMI2_2hA HEK293_TMG_2hB ELK3 protein_coding protein_coding 2.321743 1.081079 3.445616 0.03112264 0.1860909 1.663187 1.99598863 0.6398996 3.14084474 0.1991646 0.13434971 2.277450 0.84097083 0.6004667 0.91270000 0.31223333 0.49294741 0.02237223 FALSE  
ENST00000547249 ENSG00000111145 HEK293_OSMI2_2hA HEK293_TMG_2hB ELK3 protein_coding protein_coding 2.321743 1.081079 3.445616 0.03112264 0.1860909 1.663187 0.13310594 0.0000000 0.27082664 0.0000000 0.08451158 4.811608 0.03532917 0.0000000 0.07633333 0.07633333 0.02237223 0.02237223    
ENST00000549529 ENSG00000111145 HEK293_OSMI2_2hA HEK293_TMG_2hB ELK3 protein_coding processed_transcript 2.321743 1.081079 3.445616 0.03112264 0.1860909 1.663187 0.05155989 0.2374971 0.00000000 0.2374971 0.00000000 -4.629340 0.04127083 0.2080667 0.00000000 -0.20806667 0.64584751 0.02237223    
MSTRG.7832.2 ENSG00000111145 HEK293_OSMI2_2hA HEK293_TMG_2hB ELK3 protein_coding   2.321743 1.081079 3.445616 0.03112264 0.1860909 1.663187 0.05546161 0.2036824 0.03394506 0.2036824 0.03394506 -2.281695 0.05052500 0.1914667 0.01093333 -0.18053333 0.80261295 0.02237223 TRUE  
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000111145 E001 1.977033 0.009344355 0.427647152 0.57051040 12 96194375 96194395 21 + 0.449 0.359 -0.491
ENSG00000111145 E002 2.124282 0.009450729 0.352974151 0.49722853 12 96194396 96194401 6 + 0.473 0.359 -0.617
ENSG00000111145 E003 7.237350 0.002342641 0.215864780 0.34544309 12 96194402 96194544 143 + 0.870 0.789 -0.318
ENSG00000111145 E004 9.636574 0.001785750 0.465599806 0.60526920 12 96194545 96194613 69 + 0.965 0.971 0.023
ENSG00000111145 E005 11.642866 0.001622455 0.320771244 0.46393622 12 96194614 96194705 92 + 1.043 1.027 -0.057
ENSG00000111145 E006 1.030971 0.014613616 0.097014493 0.18591607 12 96198026 96198142 117 + 0.333 0.001 -9.260
ENSG00000111145 E007 0.000000       12 96212725 96212841 117 +      
ENSG00000111145 E008 0.000000       12 96213751 96213868 118 +      
ENSG00000111145 E009 21.554502 0.001160322 0.007450248 0.02238548 12 96223565 96223773 209 + 1.317 1.181 -0.480
ENSG00000111145 E010 19.726110 0.002487467 0.162645355 0.27860026 12 96246940 96247130 191 + 1.258 1.233 -0.090
ENSG00000111145 E011 14.340842 0.005494696 0.162539594 0.27845277 12 96247131 96247283 153 + 1.131 1.077 -0.192
ENSG00000111145 E012 19.457044 0.003263916 0.147771046 0.25865225 12 96247284 96247602 319 + 1.250 1.216 -0.119
ENSG00000111145 E013 16.728775 0.001147656 0.572891857 0.69892548 12 96247603 96247734 132 + 1.171 1.216 0.160
ENSG00000111145 E014 0.000000       12 96255460 96255550 91 +      
ENSG00000111145 E015 15.727463 0.018909087 0.785748143 0.86275272 12 96259731 96259853 123 + 1.141 1.200 0.212
ENSG00000111145 E016 150.923304 0.048313464 0.027864960 0.06774985 12 96267082 96269824 2743 + 2.051 2.264 0.715