ENSG00000109756

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000505026 ENSG00000109756 HEK293_OSMI2_2hA HEK293_TMG_2hB RAPGEF2 protein_coding processed_transcript 5.143317 2.615515 6.61887 0.2822684 0.4506662 1.336162 0.4614937 0.34721951 0.2865981 0.16929200 0.28659810 -0.2683015 0.09908750 0.122200000 0.03813333 -0.08406667 0.2798104650 0.0003485411 FALSE FALSE
ENST00000505478 ENSG00000109756 HEK293_OSMI2_2hA HEK293_TMG_2hB RAPGEF2 protein_coding processed_transcript 5.143317 2.615515 6.61887 0.2822684 0.4506662 1.336162 0.3173444 0.00000000 0.2641447 0.00000000 0.26414471 4.7768657 0.05112500 0.000000000 0.03516667 0.03516667 0.9545259736 0.0003485411 FALSE TRUE
ENST00000644902 ENSG00000109756 HEK293_OSMI2_2hA HEK293_TMG_2hB RAPGEF2 protein_coding protein_coding 5.143317 2.615515 6.61887 0.2822684 0.4506662 1.336162 2.2045493 1.65165067 2.2674758 0.04702268 0.09673517 0.4548186 0.46024583 0.644000000 0.34746667 -0.29653333 0.0050744471 0.0003485411 FALSE TRUE
ENST00000691494 ENSG00000109756 HEK293_OSMI2_2hA HEK293_TMG_2hB RAPGEF2 protein_coding protein_coding 5.143317 2.615515 6.61887 0.2822684 0.4506662 1.336162 1.2327358 0.58682737 1.9934565 0.10751162 0.09190748 1.7471055 0.23770833 0.223566667 0.30513333 0.08156667 0.4383626471 0.0003485411 FALSE TRUE
MSTRG.25670.3 ENSG00000109756 HEK293_OSMI2_2hA HEK293_TMG_2hB RAPGEF2 protein_coding   5.143317 2.615515 6.61887 0.2822684 0.4506662 1.336162 0.5191107 0.02194528 0.9561353 0.01384002 0.23698286 4.9185505 0.07850417 0.007533333 0.14753333 0.14000000 0.0003485411 0.0003485411 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000109756 E001 0.4439371 0.0215675176 3.567455e-01 5.011410e-01 4 159103013 159103078 66 + 0.198 0.000 -9.450
ENSG00000109756 E002 20.4585039 0.0094221506 1.765563e-02 4.639508e-02 4 159103079 159104084 1006 + 1.343 1.152 -0.671
ENSG00000109756 E003 3.8687060 0.0047100596 3.662268e-01 5.105679e-01 4 159104085 159104162 78 + 0.686 0.574 -0.490
ENSG00000109756 E004 3.8300980 0.0042858406 1.645098e-01 2.810395e-01 4 159104163 159104177 15 + 0.703 0.505 -0.883
ENSG00000109756 E005 6.9397343 0.0024634993 1.303129e-03 4.987452e-03 4 159104178 159104231 54 + 0.959 0.505 -1.883
ENSG00000109756 E006 9.8205610 0.0017140187 7.993191e-04 3.257230e-03 4 159186642 159186712 71 + 1.086 0.685 -1.541
ENSG00000109756 E007 12.8161415 0.0014871993 3.155359e-03 1.072354e-02 4 159193200 159193256 57 + 1.173 0.880 -1.075
ENSG00000109756 E008 14.2869461 0.0273395309 1.697800e-02 4.488629e-02 4 159210500 159210583 84 + 1.216 0.938 -1.011
ENSG00000109756 E009 0.0000000       4 159228254 159228357 104 +      
ENSG00000109756 E010 0.0000000       4 159229337 159229530 194 +      
ENSG00000109756 E011 16.4292239 0.0243997628 1.753831e-02 4.612324e-02 4 159238809 159238884 76 + 1.269 1.013 -0.918
ENSG00000109756 E012 0.0000000       4 159241023 159241122 100 +      
ENSG00000109756 E013 22.5191002 0.0008896184 1.006125e-03 3.979644e-03 4 159241201 159241368 168 + 1.393 1.152 -0.848
ENSG00000109756 E014 7.1934422 0.0024800014 1.952476e-01 3.202960e-01 4 159243774 159243791 18 + 0.910 0.774 -0.529
ENSG00000109756 E015 0.0000000       4 159266881 159266958 78 +      
ENSG00000109756 E016 0.0000000       4 159267161 159267309 149 +      
ENSG00000109756 E017 0.1451727 0.0430939723 1.000000e+00   4 159267737 159268215 479 + 0.077 0.000 -10.240
ENSG00000109756 E018 0.0000000       4 159282504 159282640 137 +      
ENSG00000109756 E019 25.3015497 0.0007734060 1.405478e-03 5.324313e-03 4 159304342 159304473 132 + 1.432 1.214 -0.759
ENSG00000109756 E020 33.6705676 0.0369240135 3.192678e-02 7.571443e-02 4 159314591 159314768 178 + 1.546 1.352 -0.667
ENSG00000109756 E021 28.2476080 0.0007233967 5.078930e-02 1.105823e-01 4 159322347 159322483 137 + 1.453 1.351 -0.354
ENSG00000109756 E022 29.6479277 0.0095672924 3.464248e-02 8.097465e-02 4 159323459 159323617 159 + 1.484 1.342 -0.492
ENSG00000109756 E023 0.3030308 0.3861810292 7.295130e-01   4 159326661 159329857 3197 + 0.142 0.000 -10.279
ENSG00000109756 E024 33.1083218 0.0110040645 1.288066e-01 2.324910e-01 4 159329858 159330010 153 + 1.518 1.430 -0.303
ENSG00000109756 E025 28.8143813 0.0013715711 3.269992e-02 7.720176e-02 4 159330334 159330498 165 + 1.468 1.351 -0.404
ENSG00000109756 E026 0.0000000       4 159330499 159330653 155 +      
ENSG00000109756 E027 23.5747027 0.0008322324 9.025351e-02 1.755198e-01 4 159331431 159331538 108 + 1.376 1.282 -0.328
ENSG00000109756 E028 0.0000000       4 159331626 159331629 4 +      
ENSG00000109756 E029 31.9799231 0.0018107451 3.166630e-04 1.447307e-03 4 159331630 159331833 204 + 1.534 1.306 -0.787
ENSG00000109756 E030 22.0507382 0.0114281453 1.333436e-02 3.670029e-02 4 159331926 159332034 109 + 1.376 1.169 -0.728
ENSG00000109756 E031 34.2375164 0.0022221802 1.190571e-02 3.330823e-02 4 159332451 159332697 247 + 1.546 1.410 -0.465
ENSG00000109756 E032 0.0000000       4 159332698 159332856 159 +      
ENSG00000109756 E033 30.4746184 0.0006812711 5.129613e-02 1.114617e-01 4 159338311 159338468 158 + 1.487 1.391 -0.331
ENSG00000109756 E034 43.4323625 0.0005315544 1.227213e-01 2.238403e-01 4 159339114 159339354 241 + 1.620 1.571 -0.167
ENSG00000109756 E035 49.7222733 0.0005685592 7.503426e-01 8.370473e-01 4 159341564 159341947 384 + 1.659 1.683 0.084
ENSG00000109756 E036 40.9897129 0.0005626236 2.665689e-01 4.047883e-01 4 159342979 159343190 212 + 1.595 1.571 -0.083
ENSG00000109756 E037 19.6535542 0.0013417200 5.863502e-01 7.099313e-01 4 159343281 159343293 13 + 1.255 1.340 0.297
ENSG00000109756 E038 33.4034030 0.0006988678 7.402776e-02 1.499640e-01 4 159343294 159343404 111 + 1.524 1.446 -0.269
ENSG00000109756 E039 12.0078757 0.0014491787 9.361121e-03 2.718452e-02 4 159344036 159344059 24 + 1.138 0.880 -0.950
ENSG00000109756 E040 32.0144550 0.0084420532 2.335698e-03 8.261750e-03 4 159345106 159345215 110 + 1.539 1.327 -0.732
ENSG00000109756 E041 28.9165526 0.0011082889 1.919483e-01 3.162144e-01 4 159345216 159345329 114 + 1.453 1.401 -0.181
ENSG00000109756 E042 38.2193248 0.0008884982 2.005823e-01 3.269327e-01 4 159346789 159346998 210 + 1.569 1.531 -0.131
ENSG00000109756 E043 27.4735549 0.0007625990 8.818642e-01 9.283141e-01 4 159350137 159350227 91 + 1.406 1.437 0.106
ENSG00000109756 E044 19.8248290 0.0009070484 3.729252e-01 5.173480e-01 4 159350228 159350289 62 + 1.290 1.256 -0.121
ENSG00000109756 E045 14.6847290 0.0017824673 1.631238e-01 2.792240e-01 4 159351059 159351133 75 + 1.178 1.079 -0.357
ENSG00000109756 E046 5.8512346 0.0315768174 9.914224e-01 9.987110e-01 4 159351134 159351181 48 + 0.779 0.817 0.150
ENSG00000109756 E047 0.4396707 0.0297372930 3.587465e-01 5.031166e-01 4 159352457 159352684 228 + 0.198 0.000 -11.865
ENSG00000109756 E048 41.2295466 0.0025914900 9.037480e-01 9.427559e-01 4 159352685 159352910 226 + 1.574 1.609 0.120
ENSG00000109756 E049 66.6255419 0.0005991128 6.423480e-02 1.337601e-01 4 159353487 159354046 560 + 1.746 1.873 0.427
ENSG00000109756 E050 34.6253015 0.0006434677 6.002667e-03 1.861678e-02 4 159355853 159356158 306 + 1.438 1.647 0.716
ENSG00000109756 E051 259.3253482 0.0037738014 8.918490e-36 2.286308e-33 4 159358114 159360174 2061 + 2.204 2.625 1.405