ENSG00000109689

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000463501 ENSG00000109689 HEK293_OSMI2_2hA HEK293_TMG_2hB STIM2 protein_coding protein_coding 8.650978 6.606446 10.45211 0.8975339 0.3414685 0.6610454 1.5943605 0.7694254 2.0489612 0.11471039 0.32983068 1.4014339 0.18089167 0.11636667 0.19450000 0.07813333 1.398499e-01 9.673231e-12 FALSE FALSE
ENST00000467087 ENSG00000109689 HEK293_OSMI2_2hA HEK293_TMG_2hB STIM2 protein_coding protein_coding 8.650978 6.606446 10.45211 0.8975339 0.3414685 0.6610454 1.0621474 0.6225066 2.6215147 0.20399730 0.24565311 2.0567410 0.11949167 0.10166667 0.25220000 0.15053333 9.058517e-02 9.673231e-12 FALSE TRUE
ENST00000478425 ENSG00000109689 HEK293_OSMI2_2hA HEK293_TMG_2hB STIM2 protein_coding processed_transcript 8.650978 6.606446 10.45211 0.8975339 0.3414685 0.6610454 0.3117558 0.7144705 0.2887961 0.71447049 0.28879607 -1.2777657 0.02998333 0.08623333 0.02876667 -0.05746667 9.584618e-01 9.673231e-12   FALSE
ENST00000504511 ENSG00000109689 HEK293_OSMI2_2hA HEK293_TMG_2hB STIM2 protein_coding retained_intron 8.650978 6.606446 10.45211 0.8975339 0.3414685 0.6610454 0.4749691 0.2090932 0.6848475 0.01596429 0.05033142 1.6651520 0.05251250 0.03240000 0.06593333 0.03353333 1.023559e-01 9.673231e-12   FALSE
MSTRG.24717.12 ENSG00000109689 HEK293_OSMI2_2hA HEK293_TMG_2hB STIM2 protein_coding   8.650978 6.606446 10.45211 0.8975339 0.3414685 0.6610454 0.6948534 0.0000000 1.4154350 0.00000000 0.25322991 7.1552584 0.06989167 0.00000000 0.13440000 0.13440000 9.673231e-12 9.673231e-12 FALSE TRUE
MSTRG.24717.3 ENSG00000109689 HEK293_OSMI2_2hA HEK293_TMG_2hB STIM2 protein_coding   8.650978 6.606446 10.45211 0.8975339 0.3414685 0.6610454 0.3448745 1.0575079 0.0000000 0.38866067 0.00000000 -6.7381029 0.05378333 0.17983333 0.00000000 -0.17983333 1.613936e-06 9.673231e-12   FALSE
MSTRG.24717.5 ENSG00000109689 HEK293_OSMI2_2hA HEK293_TMG_2hB STIM2 protein_coding   8.650978 6.606446 10.45211 0.8975339 0.3414685 0.6610454 2.3874416 1.7288031 2.4621661 0.27741506 0.24763659 0.5076811 0.26687917 0.26156667 0.23553333 -0.02603333 8.004550e-01 9.673231e-12 FALSE TRUE
MSTRG.24717.6 ENSG00000109689 HEK293_OSMI2_2hA HEK293_TMG_2hB STIM2 protein_coding   8.650978 6.606446 10.45211 0.8975339 0.3414685 0.6610454 1.1982360 1.1233899 0.2555641 0.59555411 0.14680834 -2.0935122 0.16099167 0.16100000 0.02366667 -0.13733333 6.539468e-01 9.673231e-12   FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000109689 E001 0.669547 0.0176809467 8.365643e-02 1.652758e-01 4 26857601 26857623 23 + 0.088 0.383 2.652
ENSG00000109689 E002 0.816796 0.0155185439 2.095039e-01 3.379473e-01 4 26857624 26857631 8 + 0.161 0.383 1.652
ENSG00000109689 E003 1.367195 0.0104118162 8.344282e-02 1.649501e-01 4 26857632 26857677 46 + 0.224 0.526 1.806
ENSG00000109689 E004 1.473758 0.1006538959 1.166514e-01 2.151805e-01 4 26857678 26857682 5 + 0.224 0.532 1.836
ENSG00000109689 E005 1.963691 0.0071909011 2.089513e-01 3.372655e-01 4 26857683 26857738 56 + 0.371 0.583 1.068
ENSG00000109689 E006 2.436672 0.0065585970 9.698911e-02 1.858841e-01 4 26857739 26857744 6 + 0.410 0.678 1.261
ENSG00000109689 E007 3.353512 0.0745646409 1.045585e-02 2.987806e-02 4 26857745 26857748 4 + 0.410 0.874 2.041
ENSG00000109689 E008 3.357779 0.0536456897 6.737658e-03 2.054238e-02 4 26857749 26857768 20 + 0.411 0.875 2.047
ENSG00000109689 E009 3.176074 0.0523991308 1.140607e-02 3.213200e-02 4 26857769 26857775 7 + 0.411 0.847 1.939
ENSG00000109689 E010 28.944394 0.0026008589 5.431530e-12 1.180166e-10 4 26857928 26858198 271 + 1.223 1.691 1.613
ENSG00000109689 E011 19.662159 0.0010376956 2.220262e-08 2.566646e-07 4 26858199 26858247 49 + 1.080 1.513 1.521
ENSG00000109689 E012 28.405250 0.0083820013 4.363043e-13 1.134768e-11 4 26858248 26858347 100 + 1.147 1.717 1.973
ENSG00000109689 E013 24.531707 0.0072582356 4.230207e-26 5.088254e-24 4 26858613 26858919 307 + 0.835 1.742 3.214
ENSG00000109689 E014 1.925071 0.0076200158 5.882572e-01 7.114602e-01 4 26860794 26860840 47 + 0.480 0.383 -0.515
ENSG00000109689 E015 7.094930 0.0119201191 4.609165e-01 6.009046e-01 4 26860841 26860982 142 + 0.925 0.824 -0.387
ENSG00000109689 E016 21.553516 0.0009048065 2.548981e-05 1.542309e-04 4 26860983 26861369 387 + 1.436 1.073 -1.278
ENSG00000109689 E017 0.000000       4 26861459 26861674 216 +      
ENSG00000109689 E018 0.000000       4 26881513 26881609 97 +      
ENSG00000109689 E019 32.678251 0.0074650426 3.629313e-05 2.113041e-04 4 26919504 26919634 131 + 1.606 1.267 -1.170
ENSG00000109689 E020 41.329931 0.0084073085 2.491602e-03 8.739452e-03 4 26957612 26957726 115 + 1.681 1.453 -0.778
ENSG00000109689 E021 47.823153 0.0193501120 2.381134e-03 8.401286e-03 4 26995379 26995490 112 + 1.755 1.485 -0.922
ENSG00000109689 E022 51.964389 0.0126192664 5.690862e-03 1.779292e-02 4 26999232 26999347 116 + 1.772 1.562 -0.714
ENSG00000109689 E023 75.054078 0.0018415705 1.643524e-06 1.302165e-05 4 27002217 27002394 178 + 1.933 1.700 -0.786
ENSG00000109689 E024 45.181186 0.0005249318 1.324664e-03 5.058629e-03 4 27002927 27002999 73 + 1.703 1.519 -0.625
ENSG00000109689 E025 51.088459 0.0007999830 2.037751e-03 7.343883e-03 4 27003000 27003104 105 + 1.750 1.583 -0.569
ENSG00000109689 E026 55.726670 0.0004663550 4.187575e-01 5.619990e-01 4 27007533 27007700 168 + 1.749 1.707 -0.139
ENSG00000109689 E027 1.772524 0.0082372342 7.745484e-01 8.546996e-01 4 27007983 27008006 24 + 0.410 0.460 0.262
ENSG00000109689 E028 45.826203 0.0096581214 1.617468e-01 2.773976e-01 4 27008428 27008528 101 + 1.687 1.584 -0.349
ENSG00000109689 E029 63.655880 0.0023095360 2.597856e-01 3.972428e-01 4 27008764 27009002 239 + 1.812 1.754 -0.196
ENSG00000109689 E030 75.412089 0.0004003403 3.376431e-01 4.815335e-01 4 27017711 27017984 274 + 1.880 1.838 -0.140
ENSG00000109689 E031 38.676156 0.0154050003 2.492341e-03 8.741719e-03 4 27019400 27021000 1601 + 1.662 1.399 -0.902
ENSG00000109689 E032 6.839523 0.0327250175 7.711532e-02 1.548642e-01 4 27021001 27021068 68 + 0.959 0.680 -1.096
ENSG00000109689 E033 7.785135 0.0031980752 8.082583e-03 2.398980e-02 4 27021282 27021493 212 + 1.028 0.678 -1.358
ENSG00000109689 E034 6.205464 0.0025766428 1.059591e-02 3.020092e-02 4 27021494 27021612 119 + 0.948 0.583 -1.476
ENSG00000109689 E035 308.420637 0.0006903394 9.002512e-09 1.119020e-07 4 27022519 27025381 2863 + 2.429 2.542 0.376